Nanozyme engineering in structurally explicit framework: Design mechanisms and biosensing applications

Biomimetic Electrochemical Sensor Based on Single-Atom Nickel Laccase Nanoenzyme for Quercetin Detection

“…12,13 Therefore, nanoenzymes have been applied in various fields ranging from biosensing, disease diagnosis, and tumor treatment to environmental protection. 14,15 At present, the reported laccase nanoenzymes are mainly based on nanomaterials with copper as the catalytic active site. 10,16 Moreover, the design and synthesis of copper-based laccase nanoenzymes mostly rely on an empirical trial and error approach involving the use of expensive raw materials and cumbersome and complex processes.…”

“…Nanoenzymes are nanomaterials with enzymatic activities, which have several advantages over natural enzymes, including high catalytic stability, large specific surface area, and easy modification. , Therefore, nanoenzymes have been applied in various fields ranging from biosensing, disease diagnosis, and tumor treatment to environmental protection. , At present, the reported laccase nanoenzymes are mainly based on nanomaterials with copper as the catalytic active site. , Moreover, the design and synthesis of copper-based laccase nanoenzymes mostly rely on an empirical trial and error approach involving the use of expensive raw materials and cumbersome and complex processes. The products are mostly amorphous, with poor mechanical strength, low catalytic activity, and limited applications.…”

Nano-enzyme hydrogels for cartilage repair effectiveness based on ternary strategy therapy

“…[42][43][44][45] However, recent attempts at artificial nano-enzymes/MOF engineering in phase studies have generally focused on cancer therapy, and biosensing and antimicrobial activities. [46][47][48] Besides, the expensive prices, low natural reserves, and large biotoxicity of the heavy, precious, and rare metals used in the current design of nano-enzymes greatly limit their application in biomedical engineering. 12 It is urgent and challenging to find highly safe and abundant nano-enzymatic/ MOF-engineered materials to expand the application for OA therapy.…”