Napsin A, a member of the aspartic protease family, is abundantly expressed in normal lung and kidney tissue and is expressed in lung adenocarcinomas

Chuman, Yoshiko; Bergman, Anders; Ueno, Takayuki; Saito, Shinichi; Sakaguchi, Kazuyasu; Alaiya, Ayodele; Franzén, Bo; Bergman, Tomas; Arnott, David; Auer, Gert; Appella, Ettore; Jörnvall, Hans; Linder, Stig

doi:10.1016/s0014-5793(99)01493-3

Cited by 99 publications

(86 citation statements)

References 16 publications

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“…2C Middle) mostly encoded small airway-associated proteins and immunologically related proteins. The presence of genes for surfactants A2 and B, pronapsin A, and mucin1 in the cluster reflects the origin of tumors derived from small airway epithelial cells, such as type 2 pneumocytes and Clara cells (36,37). However, high expression of these genes also suggested that these proteins may participate in the tumorigenesis of lung adenocarcinomas.…”

Section: Hierarchical Clustering Of Tumor and Normal Lung Tissues Basmentioning

confidence: 98%

Molecular characteristics of non-small cell lung cancer

Nacht¹,

Dracheva²,

Gao³

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

113

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We used hierarchical clustering to examine gene expression profiles generated by serial analysis of gene expression (SAGE) in a total of nine normal lung epithelial cells and non-small cell lung cancers. Separation of normal and tumor, as well as histopathological subtypes, was evident by using the 3,921 most abundant transcript tags. This distinction remained when only 115 highly differentially expressed tags were used. Furthermore, these 115 transcript tags clustered into groups suggestive of the unique biological and pathological features of the different tissues examined. Adenocarcinomas were characterized by high-level expression of small airway-associated or immunologically related proteins, whereas squamous cell carcinomas overexpressed genes involved in cellular detoxification or antioxidation. The messages of two p53-regulated genes, p21 WAF1/CIP1 and 14-3-3, were consistently underexpressed in the adenocarcinomas, suggesting that the p53 pathway itself might be compromised in this cancer type. Gene expression patterns observed by SAGE were consistent with results obtained by quantitative real-time PCR or cDNA array analyses by using a total of 43 lung tumor and normal samples. Thus, although derived from only a few tissue libraries, gene expression profiles obtained by using SAGE most likely represent an unbiased yet distinctive molecular signature for the most common forms of human lung cancer.

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Section: Hierarchical Clustering Of Tumor and Normal Lung Tissues Basmentioning

confidence: 98%

Molecular characteristics of non-small cell lung cancer

Nacht¹,

Dracheva²,

Gao³

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

113

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“…[1][2][3][4] Napsin A has a role in processing pulmonary surfactant B protein produced by alveolar type II pneumocytes; 5,6 thus, it has been reported to be a good diagnostic marker for the confirmation of primary lung adenocarcinoma together with thyroid transcription factor 1 (TTF-1). [7][8][9][10][11][12][13] Expression of napsin A is also observed in the fetal kidney 14 and proximal tubules of the adult kidney, where it is most likely involved in protein catabolism.…”

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confidence: 99%

Napsin A is a specific marker for ovarian clear cell adenocarcinoma

et al. 2015

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Ovarian clear cell adenocarcinoma has a relatively poor prognosis among the ovarian cancer subtypes because of its high chemoresistance. Differential diagnosis of clear cell adenocarcinoma from other ovarian surface epithelial tumors is important for its treatment. Napsin A is a known diagnostic marker for lung adenocarcinoma, and expression of napsin A is reported in a certain portion of thyroid and renal carcinomas. However, napsin A expression in ovarian surface epithelial tumors has not previously been examined. In this study, immunohistochemical analysis revealed that in 71 of 86 ovarian clear cell adenocarcinoma patients (83%) and all of the 13 patients with ovarian clear cell adenofibroma, positive napsin A staining was evident. No expression was observed in 30 serous adenocarcinomas, 11 serous adenomas or borderline tumors, 19 endometrioid adenocarcinomas, 22 mucinous adenomas or borderline tumors, 10 mucinous adenocarcinomas, or 3 yolk sac tumors of the ovary. Furthermore, expression of napsin A was not observed in the normal surface epithelium of the ovary, epithelia of the fallopian tubes, squamous epithelium, endocervical epithelium, or the endometrium of the uterus. Therefore, we propose that napsin A is another sensitive and specific marker for distinguishing ovarian clear cell tumors (especially adenocarcinomas) from other ovarian tumors.

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“…Some tumour-specific markers for clinical use have been identified by proteomic studies, for example TAO1 and TAO2, are two effective markers for distinguishing primary lung adenocarcinoma from metastasised adenocarcinoma originating in other organs Hirano et al, 1997). These proteins were both later identified as napsin A (Chuman et al, 1999). Furthermore, cytokeratins and vimentin are used in the diagnosis of lung cancer and lower intestinal cancer (Cathro and Stoler, 2002;Liu et al, 2004).…”

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confidence: 99%

Differential tissue-specific protein markers of vaginal carcinoma

et al. 2009

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The objective was to identify proteins differentially expressed in vaginal cancer to elucidate relevant cancer-related proteins. A total of 16 fresh-frozen tissue biopsies, consisting of 5 biopsies from normal vaginal epithelium, 6 from primary vaginal carcinomas and 5 from primary cervical carcinomas, were analysed using two-dimensional gel electrophoresis (2-DE) and MALDI-TOF mass spectrometry. Of the 43 proteins identified with significant alterations in protein expression between non-tumourous and tumourous tissue, 26 were upregulated and 17 were downregulated. Some were similarly altered in vaginal and cervical carcinoma, including cytoskeletal proteins, tumour suppressor proteins, oncoproteins implicated in apoptosis and proteins in the ubiquitin -proteasome pathway. Three proteins were uniquely altered in vaginal carcinoma (DDX48, erbB3-binding protein and biliverdin reductase) and five in cervical carcinoma (peroxiredoxin 2, annexin A2, sarcomeric tropomyosin kappa, human ribonuclease inhibitor and prolyl-4-hydrolase beta). The identified proteins imply involvement of multiple different cellular pathways in the carcinogenesis of vaginal carcinoma. Similar protein alterations were found between vaginal and cervical carcinoma suggesting common tumourigenesis. However, the expression level of some of these proteins markedly differs among the three tissue specimens indicating that they might be useful molecular markers.

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Napsin A, a member of the aspartic protease family, is abundantly expressed in normal lung and kidney tissue and is expressed in lung adenocarcinomas

Cited by 99 publications

References 16 publications

Molecular characteristics of non-small cell lung cancer

Molecular characteristics of non-small cell lung cancer

Napsin A is a specific marker for ovarian clear cell adenocarcinoma

Differential tissue-specific protein markers of vaginal carcinoma

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