Narcolepsy type 1

Swarnakar, Rajesh; Deotare, Akshay

doi:10.4103/lungindia.lungindia_389_15

Cited by 1 publication

(1 citation statement)

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“…Narcolepsy is a neurological sleep disorder characterized by a pentad of symptoms: excessive daytime sleepiness, cataplexy, hypnagogic and hypnopompic hallucinations, sleep paralysis (especially on awakening), and disturbed nocturnal sleep [1]. The prevalence of narcolepsy with cataplexy ranges between 25 and 50 per 100,000 individuals [2,3]. The two primary symptoms of narcolepsy type 1 (NT1) are excessive daytime sleepiness and cataplexy, which is a sudden loss of muscle tone while awake, usually triggered by powerful, typically positive emotions.…”

Section: Introductionmentioning

confidence: 99%

LC-MS/MS Quantitation of HILIC-Enriched N-glycopeptides Derived from Low-Abundance Serum Glycoproteins in Patients with Narcolepsy Type 1

Atashi,

Reyes,

Sandilya

et al. 2023

Biomolecules

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Glycoproteomic analysis is always challenging because of low abundance and complex site-specific heterogeneity. Glycoproteins are involved in various biological processes such as cell signaling, adhesion, and cell–cell communication and may serve as potential biomarkers when analyzing different diseases. Here, we investigate glycoproteins in narcolepsy type 1 (NT1) disease, a form of narcolepsy characterized by cataplexy—the sudden onset of muscle paralysis that is typically triggered by intense emotions. In this study, 27 human blood serum samples were analyzed, 16 from NT1 patients and 11 from healthy individuals serving as controls. We quantified hydrophilic interaction liquid chromatography (HILIC)-enriched glycopeptides from low-abundance serum samples of controls and NT1 patients via LC-MS/MS. Twenty-eight unique N-glycopeptides showed significant changes between the two studied groups. The sialylated N-glycopeptide structures LPTQNITFQTESSVAEQEAEFQSPK HexNAc6, Hex3, Neu5Ac2 (derived from the ITIH4 protein) and the structure IVLDPSGSMNIYLVLDGSDSIGASNFTGAK HexNAc5, Hex4, Fuc1 (derived from the CFB protein), with p values of 0.008 and 0.01, respectively, were elevated in NT1 samples compared with controls. In addition, the N-glycopeptide protein sources Ceruloplasmin, Complement factor B, and ITH4 were observed to play an important role in the complement activation and acute-phase response signaling pathways. This may explain the possible association between the biomarkers and pathophysiological effects.

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Section: Introductionmentioning

confidence: 99%