Naringen Ameliorates Cyanide-induced Testicular and Epididymal Changes in Swiss Albino Mice (&lt;I&gt;Mus musculus&lt;/I&gt;)

Sanni, Ambeliou; Idaguko, C.A.; Abdulazeez, D.O.; Adeleke, Opeyemi; Falana, Benedict Abiola

doi:10.4314/rejhs.v8i4.9

Cited by 4 publications

(4 citation statements)

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“…The morphometric data obtained from this study indicate that the animals’ body weight and testicular weight were significant reduced in cyanide treated groups while cyanide co-administered with quercetin treated groups showed significant weight gain. This result is in line with Sanni et al. (2020) who reported significant weight loss in rats treated with cyanide and also with Rezaei-Sadabady et al.…”

Section: Discussionsupporting

confidence: 91%

Quercetin upregulates CREM gene expression in cyanide-induced endocrine dysfunction

Oyewopo

Adeleke

Johnson

et al. 2021

Heliyon

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Cyanide is among the ubiquitous chemicals that humans are usually exposed to and it is well documented that cyanide induces infertility in humans and experimental rodents. However, the pathogenesis remains unknown. Likewise, quercetin is an important nutraceutical that detoxifies reactive oxygen species, but its effects on testicular damage is not clear. The present study investigated the role of nutraceutical, quercetin on cyanide-induced testicular toxicity and probable involvement of cAMP-response-element modulator (CREM) which is a transcription factor necessary for the process of spermatogenesis. Thus, this work hypothesized that quercetin will mitigate endocrine dysfunction induced by cyanide. Seventy-two adult male Wistar rats were divided into seven groups (A to G). Groups A, B, C, F and G comprised of eight (8) rats per group while groups D and E comprised of sixteen (16) rats per group. Group A was designated as control while Groups B and C were given 0.5 and 1 mg/kg of cyanide respectively for 56 days. Group D and E received 0.5 and 1 mg/kg body weight cyanide respectively for 30 days. At day 30, eight animals were sacrificed from Group D and E and the remaining eight (8) rats were subdivided into sub-groups (D1 and E1) and were given 20 and 40 mg/kg of quercetin respectively for twenty-six (26) days. Group F and G were given concurrent administration of cyanide and quercetin at a dose of 0.5 + 20 mg/kg and 1 + 40 mg/kg respectively for 56 days. Body and testicular weight were significantly reduced in cyanide treated groups while quercetin modulates the reduction. Significant down-regulation of CREM gene and reduction in serum level of follicle stimulating hormone (FSH), Luteinizing hormone (LH), testosterone, glutathione peroxidase (GPx) and zinc in cyanide-treated groups, whereas administration of quercetin concomitantly with cyanide exposure or post-treated significantly reversed the alterations.

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Section: Discussionsupporting

confidence: 91%

Quercetin upregulates CREM gene expression in cyanide-induced endocrine dysfunction

Oyewopo

Adeleke

Johnson

et al. 2021

Heliyon

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“…However, animals co-administered cyanide and quercetin and post-treated with quercetin had normal testicular microarchitecture. This finding is corroborated by the report of Sanni et al. (2020) on bioflavonoids.…”

Section: Discussionsupporting

confidence: 87%

“…The current study found that cyanide exposure resulted in significant decreases in epididymal sperm concentration, sperm motility (progressive), and the normal/abnormal sperm ratio. Many investigations on the reproductive system of male animals have shown that cyanide is toxic to testicular tissue and functions ( Shivanoor and David, 2015a , Shivanoor and David, 2015b ; Sanni et al., 2020 ), including significant reductions in the quantity of spermatozoa within the epididymis in cyanide-treated mice. Alterations in sperm parameters were observed following cyanide administration in previous studies ( De Sousa et al., 2007 ; Shivanoor and David, 2015a , Shivanoor and David, 2015b ).…”

Section: Discussionmentioning

confidence: 99%

Regulatory effects of quercetin on testicular histopathology induced by cyanide in Wistar rats

Oyewopo

Adeleke

Johnson

et al. 2021

Heliyon

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Several causes of infertility have been identified, and several papers have documented some compounds that cause infertility. One of the compounds reported to be toxic to the reproductive system is cyanide. In the management of infertility, various mechanisms ranging from synthetic drugs, natural products and supplements have been employed. Quercetin is an antioxidant supplement that has been used in the treatment of a variety of ailments. This work is aimed at investigating the role of quercetin in attenuating spermato-toxicity and testicular-histopathology induced by cyanide. Seventy-two (72) male wistar rat (weight 190 g ± 10 g) were divided into nine groups (n = 8) except for groups 4 and 5 with (n = 16). Group 1 (control) received physiological saline while Groups 2 and 3 received 0.5 and 1 mg/kg body weight (bwt) cyanide respectively for 56 days, groups 4 and 5 received 0.5 and 1 mg/kg bwt cyanide respectively for 30 days. At day 30, eight animals were sacrificed from Groups 4 and 5 and the remaining eight (8) rats were subdivided into groups (6 and 7) and were given 20 and 40 mg/kg bwt of quercetin respectively for twenty-six days. Co-administration of cyanide and quercetin at a dose of 0.5 mg/kg cyanide +20 mg/kg quercetin and 1 mg/kg cyanide +40 mg/kg quercetin were given to group 8 and 9 respectively for 56 days. Significant decreases in sperm parameters (count, motile and normal sperm) and increases in malondiadehyde concentration were observed in the cyanide treated groups. Testicular histoarchitecture showed few to no spermatozoa in the lumen of rats treated with cyanide. All these effects were attenuated by quercetin. In conclusion, quercetin regulates testicular histopathology induced by cyanide in Wistar rats. Data from this work suggests potential preventive or therapeutic applications of quercetin for individuals subjected to cyanide environmental pollution.

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“…Deductions made as a result of this type of one-way ANOVA test application would be misleading. We found similar errors in articles number 10 (Milojević 2020), 19 (Sanni et al 2020) and 26 (Skaik 2015).…”

Section: Discussionsupporting

confidence: 60%

Misuse of analysis of variance in African biomedical journals: a call for more vigilance

et al. 2022

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Background Misuse of analysis of variance alongside other statistical methods has been an important topic of discussion in many scientific gatherings. Although misuse of analysis of variance is of global concern, its prevalence in African-based biomedical journals has raised concerns among colleagues. Methods We sampled the current issues/last published issues of all African biomedical journals aggregated in an African journal aggregator. We scanned through all the journals’ sample articles in the journal aggregator and retrieved articles that either applied analysis of variance or were supposed to use the test. Results Our results show that 126 articles of the total 1789 sampled articles employed analysis of variance test for analysis. Of these, 99 (78.6%) articles used the analysis of variance test correctly, while 27 (21.4%) misused the test. Not stating the type of analysis of variance test employed for analysis was the most prevalent misuse of analysis of variance test. Conclusions In overall, the study demonstrated that misuse of analysis of variance test is prevalent in African biomedical journals. Therefore, all stakeholders (medical schools, reviewers, journal editors) need to do more to nip it in the bud.

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Naringen Ameliorates Cyanide-induced Testicular and Epididymal Changes in Swiss Albino Mice (<I>Mus musculus</I>)

Cited by 4 publications

References 1 publication

Quercetin upregulates CREM gene expression in cyanide-induced endocrine dysfunction

Quercetin upregulates CREM gene expression in cyanide-induced endocrine dysfunction

Regulatory effects of quercetin on testicular histopathology induced by cyanide in Wistar rats

Misuse of analysis of variance in African biomedical journals: a call for more vigilance

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