Naringin ameliorates radiation-induced hepatic damage through modulation of Nrf2 and NF-κB pathways

Manna, Krishnendu; Khan, Amitava; Biswas, Subhendu; Das, Ujjal; Sengupta, Arunava; Mukherjee, Dipanwita Das; Chakraborty, Anindita; Dey, Sanjit

doi:10.1039/c6ra01102k

Cited by 19 publications

(13 citation statements)

References 27 publications

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“…The absorbance of this supernatant was measured at 550 nm, and the results were calculated and expressed as nM of TBARS/mg protein against an appropriate blank. 27…”

Section: Determination Of Renal Lipid Peroxidation (Lpo)mentioning

confidence: 99%

“…The unstained renal section (6 µm of thickness) of all experimental groups was analyzed through indirect immunofluorescence. 27 Briefly, the sections were deparaffinized and hydrated in graded ethanol, followed by blocking (0.3% hydrogen peroxide) for 20 minutes to mask the endogenous peroxidase activity. A microwave oven was used to retrieve the antigen using citrate buffer (10 mM, pH 6.0) at temperatures ranging between 95°C and 98°C for 15 minutes, and then the section was further blocked with BSA (5%) for 30 minutes at 37°C.…”

Section: Determination Of Protein Expression Using Immunofluorescencementioning

confidence: 99%

“…The images were observed in an Olympus FV1000 MPE SIM Laser Scanning confocal microscope (Olympus Corporation, Tokyo, Japan). 27,30 The resultant intensity was analyzed by using ImageJ software.…”

Section: Determination Of Protein Expression Using Immunofluorescencementioning

confidence: 99%

“…31 The relative density of each blot was measured using ImageJ software. 27,32 Determination of proinflammatory cytokine level IL 1-beta (IL-1β), IL-2, IL-6, IL-10, and tumor necrosis factor-alpha (TNF-α) concentrations in the renal tissue samples were analyzed by using commercially available kits from eBiosciences (Santa Clara, CA, USA). Absorbance was measured at 450 nm.…”

Section: Determination Of Protein Expression Using Immunoblottingmentioning

confidence: 99%

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<p>Amelioration of diabetic nephropathy using pomegranate peel extract-stabilized gold nanoparticles: assessment of NF-κB and Nrf2 signaling system</p>

Manna

Mishra

Saha

et al. 2019

IJN

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Background: Diabetic nephropathy (DN), an end-stage renal disorder, has posed a menace to humankind globally, because of its complex nature and poorly understandable intricate mechanism. In recent times, functional foods as potential health benefits have been gaining attention of consumers and researchers alike. Rich in antioxidants, the peel and seed of pomegranate have previously demonstrated protection against oxidative-stress-related diseases, including cardiovascular disorders, diabetes, and cancer. Purpose: This study was designed to investigate the ameliorative role of pomegranate peel extract-stabilized gold nanoparticle (PPE-AuNP) on streptozotocin (STZ)-induced DN in an experimental murine model. Methods: Following the reduction methods, AuNP was prepared using the pomegranate peel ellagitannins and characterized by particle size, physical appearance, and morphological architecture. Modulatory potential of PPE-AuNP was examined through the plethora of biochemical and high throughput techniques, flow cytometry, immunoblotting, and immunofluorescence. Results: The animals treated with PPE-AuNP markedly reduced the fasting blood glucose, renal toxicity indices, and serum TC and TG in a hyperglycemic condition. As evident from an increased level of plasma insulin level, PPE-AuNP normalized the STZ-induced pancreatic β-cell dysfunction. The STZ-mediated suppression of endogenous antioxidant response was restored by the PPE-AuNP treatment, which reduced the generation of LPO as well as iROS. Furthermore, the hyperglycemia-mediated augmentation of protein glycation, followed by the NOX4/p-47 phox activation, diminished with the application of PPE-AuNP. The histological and immunohistochemical findings showed the protective efficacy of PPE-AuNP in reducing STZinduced glomerular sclerosis and renal fibrosis. In addition, it reduced proinflammatory burden through the modulation of the MAPK/NF-κB/STAT3/cytokine axis. Simultaneously, PI3K/ AKT-guided Nrf2 activation was evident upon the PPE-AuNP application, which enhanced the antioxidant response and maintained hyperglycemic homeostasis. Conclusion: The findings indicate that the use of PPE-AuNPs might act as an economic therapeutic remedy for alleviating DN.

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“…The absorbance of this supernatant was measured at 550 nm, and the results were calculated and expressed as nM of TBARS/mg protein against an appropriate blank. 27…”

Section: Determination Of Renal Lipid Peroxidation (Lpo)mentioning

confidence: 99%

Section: Determination Of Protein Expression Using Immunofluorescencementioning

confidence: 99%

Section: Determination Of Protein Expression Using Immunofluorescencementioning

confidence: 99%

Section: Determination Of Protein Expression Using Immunoblottingmentioning

confidence: 99%

See 2 more Smart Citations

<p>Amelioration of diabetic nephropathy using pomegranate peel extract-stabilized gold nanoparticles: assessment of NF-κB and Nrf2 signaling system</p>

Manna

Mishra

Saha

et al. 2019

IJN

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“…Also, NIN gets converted to naringenin, which has been shown to impart health‐promoting properties like including anti‐inflammatory and antioxidant activities . A number of reports advocate the ability of NIN to exert its antioxidant and cytoprotective property via activating Nrf2, which aids in the activation of various antioxidants such as GSH, Glutathione S‐transferase (GST) and downstream molecules that help in ameliorating against oxidative stress . Hence, we put forward that plant‐derived polyphenolic compounds will help in reducing the adverse effects of cadmium‐mediated hepatotoxicity.…”

Section: Introductionmentioning

confidence: 99%

Naringin abates adverse effects of cadmium‐mediated hepatotoxicity: An experimental study using HepG2 cells

Rathi

Das

Raghavendra

et al. 2017

J Biochem & Molecular Tox

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This study investigated the protective potential of Naringin (NIN) against cadmium chloride (CdCl ) mediated hepatotoxicity using human hepatocellular carcinoma (HepG2) cells. An optimal concentration of NIN (5 μM) was potent enough to confer cytoprotection against CdCl (50 μM) as was observed by MTT assay. Preconditioning with NIN maintained redox homeostasis, mitochondrial membrane potential, and reduced apoptosis as marked by decrease in the percentage sub-G /G and Annexin V-FITC/propidium iodide positive cells (apoptotic). NIN pretreatment maintained the levels of protein thiol along with endogenous activities of Superoxide dismutase, Glutathione S-transferase, and Catalase and lowered lipid peroxidation. Decreased Bax/Bcl2 ratio along with reduced Caspase 3 cleavage and Cytochrome c release indicated that NIN conditioning blocked mitochondrial-mediated apoptosis. Increased Nrf2 and metallothionein (MT) acted as adaptive response in the presence of cadmium. Thus, the protective mechanism of NIN is attributed to its antioxidant potential which aids in redox homeostasis and prevents CdCl mediated cytotoxicity.

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Screening for polystyrene nanoparticle toxicity on kidneys of adult male albino rats using histopathological, biochemical, and molecular examination results

et al. 2022

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Polystyrene Nanoparticles (PS-NPs) used for packaging foam, disposable cups, and food containers. Therefore, this study aimed to evaluate PS- NPs toxic effects on kidney of adult male albino rats. A total of 30 rats divided into three groups (n = 10): group I negative control group; group II orally administered 3% PS-NPs (3 mg/kg body weight/day) and group III orally administered 3% PS-NPs (10 mg/kg body weight/day) for 35 days. Blood and kidney samples collected and processed for biochemical, histopathological, and immunohistochemical examinations. Results showed that low and high doses PS-NPs had significantly increased serum blood urea nitrogen (BUN), creatinine, malondialdehyde, significantly further reduced glutathione, downregulation of nuclear factor erythroid 2–related factor 2 and glutathione peroxidase, upregulation of caspase-3 and Cytochrome-c. Histopathological examination revealed several alterations. Low dose of PS-NPs exhibited dilated glomerular capillaries, hypotrophy of some renal corpuscles significantly decreases their diameter to 62 μm. Some proximal convoluted tubules and distal convoluted tubules showed loss of cellular architecture with pyknotic nuclei. Hyalinization and vacuolation in renal medulla. In high dose PS-NPs, alterations increased in severity. A significant increase in percentage area of cyclooxygenase-2 in low and high-doses. In conclusion, PS-NPs are a nephrotoxic causing renal dysfunction.

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Naringin ameliorates radiation-induced hepatic damage through modulation of Nrf2 and NF-κB pathways

Abstract: Naringin ameliorates the toxic effects of radiation in murine liver and decreases the oxidative stress through the modulation of redox-regulated cellular signaling system.

Cited by 19 publications

References 27 publications

<p>Amelioration of diabetic nephropathy using pomegranate peel extract-stabilized gold nanoparticles: assessment of NF-κB and Nrf2 signaling system</p>

<p>Amelioration of diabetic nephropathy using pomegranate peel extract-stabilized gold nanoparticles: assessment of NF-κB and Nrf2 signaling system</p>

Naringin abates adverse effects of cadmium‐mediated hepatotoxicity: An experimental study using HepG2 cells

Screening for polystyrene nanoparticle toxicity on kidneys of adult male albino rats using histopathological, biochemical, and molecular examination results

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Naringin ameliorates radiation-induced hepatic damage through modulation of Nrf2 and NF-κB pathways

Abstract: Naringin ameliorates the toxic effects of radiation in murine liver and decreases the oxidative stress through the modulation of redox-regulated cellular signaling system.

Cited by 19 publications

References 27 publications

<p>Amelioration of diabetic nephropathy using pomegranate peel extract-stabilized gold nanoparticles: assessment of NF-&kappa;B and Nrf2 signaling system</p>

<p>Amelioration of diabetic nephropathy using pomegranate peel extract-stabilized gold nanoparticles: assessment of NF-&kappa;B and Nrf2 signaling system</p>

Naringin abates adverse effects of cadmium‐mediated hepatotoxicity: An experimental study using HepG2 cells

Screening for polystyrene nanoparticle toxicity on kidneys of adult male albino rats using histopathological, biochemical, and molecular examination results

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Resources

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<p>Amelioration of diabetic nephropathy using pomegranate peel extract-stabilized gold nanoparticles: assessment of NF-κB and Nrf2 signaling system</p>

<p>Amelioration of diabetic nephropathy using pomegranate peel extract-stabilized gold nanoparticles: assessment of NF-κB and Nrf2 signaling system</p>