Narrow-band polymer dots with pronounced fluorescence fluctuations for dual-color super-resolution imaging

Abstract

Narrow-band polymer dots are developed for high-order SOFI nanoscopy, yielding a resolution of ∼61 nm in subcellular imaging.

Architectures and Applications of BODIPY-Based Conjugated Polymers

“…These porous materials not only have potential capacity of iodine absorption but also provide a feasible strategy to facilitate the development of other volatile compounds. Recently, Wu and coworkers [108] designed two BODIPY-based conjugated polymers 84 and 85 ( Figure 30), and then further prepared Pdots with uniform small-size distribution for super-resolution optical fluctuation imaging (SOFI). This imaging technique is based on the random fluctuation of the fluorescence intensity of mutually fluorescent chromogens to achieve resolution improvement.…”

“…Chemical structures of polymers 84 and 85 (Reproduced with permission from Reference[108]. Copyright @ 2016, Royal Society of Chemistry).…”

Bleaching‐Resistant Super‐Resolution Fluorescence Microscopy

“…Reproduced with permission. [ 108 ] Copyright 2020, The Royal Society of Chemistry. I) STED imaging of mitochondria by TPA‐T‐CyP AIE nanoparticle (mitochondria in a live HeLa cell).…”

“…[ 104 , 105 , 106 , 200 ] Small polymer dots (PDots) exhibit spontaneous fluctuations in fluorescence that make them suited for fluctuation‐based super‐resolution techniques, such as super‐resolution optical fluctuation imaging (SOFI) (Figure 4H ). [ 107 , 108 ] Several aggregation‐induced emission (AIE) nanoparticles have large STED cross‐sections and strong resistance to photobleaching. As a result, such AIE nanoparticles are excellent candidates for long‐term STED imaging (Figure 4I ).…”

“…H) Eighth-order SOFI imaging on continuously blinking PDots (microtubules in a fixed BS-C1 cell). Reproduced with permission [108]. Copyright 2020, The Royal Society of Chemistry.…”