Nasal brushing for the study of ciliary ultrastructure.

Rutland, J; Dewar, A.; Cox, Theresa; Cole, Peter

doi:10.1136/jcp.35.3.357

Cited by 85 publications

(47 citation statements)

References 3 publications

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“…The rate of ciliary beat (mean ± SD) for the normal subjects was (14) Hz; for patients with bronchiectasis 12-2 (1 1) Hz; and for patients with primary ciliary dyskinesia 4 7 (4 8 de Iongh, Rutland In patients with primary ciliary dyskinesia the mean number of cilia examined at each ciliary level was 28 (range 12-52), 93 (43-136), and 66 (39-140), respectively. Ciliary deviations in the two patient groups were also similar among the three regions but less so than for normal subjects.…”

Section: Resultsmentioning

confidence: 99%

Orientation of respiratory tract cilia in patients with primary ciliary dyskinesia, bronchiectasis, and in normal subjects.

Iongh

Rutland

1989

Journal of Clinical Pathology

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Section: Resultsmentioning

confidence: 99%

Orientation of respiratory tract cilia in patients with primary ciliary dyskinesia, bronchiectasis, and in normal subjects.

Iongh

Rutland

1989

Journal of Clinical Pathology

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“…This is mainly because of the invasiveness and risk of most techniques aimed at collecting human cells (eg, biopsies), the small number of cells thus collected, or the limited number, poor quality, and nonrepresentative nature of samples resulting from surgery (such as nasal polypectomies or lung transplants). Brushing of the respiratory tract, however, a noninvasive method originally proposed for ciliary studies (Kelsen et al, 1992;Rutland and Cole, 1980;Rutland et al, 1982), allows the easy sampling of numerous, representative, wellpreserved, and dissociated cells from the superficial mucosa (Bridges et al, 1991;Chapelin et al, 1996;Danel et al, 1996). The present study was thus performed on cell samples obtained by nasal brushings of F508del homozygous patients with CF, F508del carriers, and non-CF control subjects.…”

Section: Discussionmentioning

confidence: 99%

Cystic Fibrosis F508del Patients Have Apically Localized CFTR in a Reduced Number of Airway Cells

Penque

Mendes

Beck

et al. 2000

Laboratory Investigation

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SUMMARY:Present state of knowledge, mostly based on heterologous expression studies, indicates that the cystic fibrosis transmembrane conductance regulator (CFTR) protein bearing the F508del mutation is misprocessed and mislocalized in the cytoplasm, unable to reach the cell surface. Recently, however, it was described that protein levels and localization are similar between F508del and wild-type CFTR in airway and intestinal tissues, but not in the sweat glands. In this study, we used immunocytochemistry with three different anti-CFTR antibodies to investigate endogenous CFTR expression and localization in nasal epithelial cells from F508del homozygous patients, F508del carriers, and non-CF individuals. On average, 300 cells were observed per individual. No significant differences were observed for cell type distributions among CF, carrier, and non-CF samples; epithelial cells made up approximately 80% to 95% of all cells present. CFTR was detected mostly in the apical region (AR) of the tall columnar epithelial (TCE) cells, ciliated or nonciliated. By confocal microscopy analysis, we show that the CFTR apical region-staining does not overlap with either anti-calnexin (endoplasmic reticulum), anti-p58 (Golgi), or anti-tubulin (cilia) stainings. The median from results with three antibodies indicate that the apical localization of CFTR happens in 22% of TCE cells from F508del homozygous patients with CF (n ϭ 12), in 42% of cells from F508del carriers (n ϭ 20), and in 56% of cells from healthy individuals (n ϭ 12). Statistical analysis indicates that differences are significant among all groups studied and for the three antibodies (p Ͻ 0.05). These results confirm the presence of CFTR in the apical region of airway cells from F508del homozygous patients; however, they also reveal that the number of cells in which this occurs is significantly lower than in F508del carriers and much lower than in healthy individuals. These findings may have an impact on the design of novel pharmacological strategies aimed at circumventing the CF defect caused by the F508del mutation. (Lab Invest 2000, 80:857-868).

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“…Most human studies are performed on biopsies, which focus on a limited area and only allow the number of each cell type to be expressed in relation to a unit area. Brushing of the respiratory tract, a noninvasive method originally proposed for ciliary studies, harvests cells in a larger area of airway mucosa than biopsy [8,9]. Recovering superficial cells from nasal mucosa by brushing is safe and easy to perform, and allows quantification of the various cell types after cytocentrifugation [10].…”

Section: Discussionmentioning

confidence: 99%

Modified epithelial cell distribution in chronic airways inflammation

Chapelin

Coste²,

Gilain³

et al. 1996

Eur Respir J

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Although airway epithelium is known to be modified during chronic respiratory diseases, epithelial cells have rarely been precisely quantified. We therefore intended to evaluate epithelial cell distribution in inflammatory airways, using a cytological approach. Nasal airway cells in 12 patients with nonallergic chronic rhinitis were sampled by brushing, quantified after cytocentrifugation and compared to those from eight controls. Cell populations were quantified after May-Grünwald Giemsa staining and α-tubulin immunolabelling to demonstrate ciliary differentiation. When compared to controls, rhinitis patients exhibited lower percentages of ciliated cells (59±4 versus 32±2%, respectively), and higher percentages of goblet (24±3 versus 37±2%) and basal cells (9±1 versus 18±2%). After tubulin immunolabelling, positive staining was specifically detected in cells with cilia (LC+), and in the cytoplasm of some small round cells without obvious cilia (LC-). Fewer immunolabelled cells were detected in rhinitis patients than in controls (with significantly lower percentages of LC+ and higher percentages of LC-).Nasal brushing is an effective technique for quantification of airway epithelial cells. Tubulin immunolabelling is useful to detect ciliated cells and distinguishes another cell population, possibly preciliated cells. These cytological findings suggest the presence of modifications of epithelial differentiation and proliferation, possibly related to local chronic inflammation.

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Nasal brushing for the study of ciliary ultrastructure.

Cited by 85 publications

References 3 publications

Orientation of respiratory tract cilia in patients with primary ciliary dyskinesia, bronchiectasis, and in normal subjects.

Orientation of respiratory tract cilia in patients with primary ciliary dyskinesia, bronchiectasis, and in normal subjects.

Cystic Fibrosis F508del Patients Have Apically Localized CFTR in a Reduced Number of Airway Cells

Modified epithelial cell distribution in chronic airways inflammation

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