Nasal carriage of Staphylococcus schleiferi from healthy dogs and dogs with otitis, pyoderma or both

Aims: This study was to investigate and to characterize methicillin-resistant coagulase-positive staphylococci (MRCoPS) harboring in dogs and people associated with dogs in Thailand. Methods and Results: Staphylococci were collected from 100 dogs, 100 dog owners, 200 small animal veterinarians and 100 people without pet association. Species of MRCoPS were identified phenotypically and genotypically. Molecular characteristics were determined by multilocus sequence typing (MLST), pulsedfield gel electrophoresis (PFGE) and SCCmec typing, and antimicrobial susceptibility was assayed by broth microdilution and by microarray analysis for resistance genes. Methicillin-resistant Staphylococcus pseudintermedius (MRSP), methicillin-resistant Staphylococcus schleiferi subsp. coagulans (MRSSc) and methicillin-resistant Staphylococcus aureus (MRSA) were isolated from dogs (45, 17 and 1%, respectively), veterinarians (8, 2 and 1Á5%, respectively) and dog owners (3, 2 and 0%, respectively). Seventeen sequence types (STs) were identified among 83 MRSP isolates which specifically carried SCCmec V, II-III, ΨSCCmec 57395 and three uncharacterized SCCmec types. MRSP ST 45, 68 and novel STs including 169, 178, 181 and 183 were shared among canine and human isolates. Most of MRSA ST398 and MRSSc carried SCCmec type V. The MRCoPS commonly displayed multiple resistances to tested antimicrobials and carried various resistance genes. Conclusion: Variety of MRCoPS, especially new MRSP clones, distributed in dogs and people in Thailand. Significance and Impact of the Study: The existence of MRCoPS circulating between dogs and humans in Thailand provides indirect evidence of interspecies transmission and represents a potential public health hazard.

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“…; May et al . ). CoPS are opportunistic pathogens that cause serious infections such as wound infection, pneumonia and bacteremia.…”

Section: Introductionmentioning

confidence: 97%

Strain typing and antimicrobial susceptibility of methicillin-resistant coagulase-positive staphylococcal species in dogs and people associated with dogs in Thailand

et al. 2014

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“…In small animals, S. schleiferi most frequently colonizes the skin, nares, ears, and rectum of dogs, where it can cause inflammatory skin disease, otitis externa, and otitis media (2,3,5,(7)(8)(9)(10)(11)(12)(13)(14). Though found at a lower prevalence than that in dogs, S. schleiferi has also been isolated from healthy cats (1), cats with inflammatory skin disease (1), and parrots (2).…”

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confidence: 99%

Evaluation of Oxacillin and Cefoxitin Disk Diffusion and MIC Breakpoints Established by the Clinical and Laboratory Standards Institute for Detection of mecA -Mediated Oxacillin Resistance in Staphylococcus schleiferi

et al. 2018

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is a beta-hemolytic, coagulase-variable colonizer of small animals that can cause opportunistic infections in humans. In veterinary isolates, the rate of -mediated oxacillin resistance is significant, with reported resistance rates of>39%. The goal of this study was to evaluate oxacillin and cefoxitin disk diffusion (DD) and MIC breakpoints for detection of -mediated oxacillin resistance in 52 human and 38 veterinary isolates of Isolates were tested on multiple brands of commercial media and according to Clinical and Laboratory Standards Institute (CLSI) methods. Zone diameters and MIC values were interpreted using CLSI breakpoints (CLSI, , 2017) for/, coagulase-negative staphylococci (CoNS), and Results were compared to those of PCR. Twenty-nine of 90 (32%) isolates were positive. Oxacillin inhibition zone sizes and MICs interpreted by breakpoints reliably differentiated -positive and-negative isolates, with a categorical agreement (CA) of 100% and no very major errors (VMEs) or major errors (MEs) for all media. For cefoxitin DD results interpreted using / and CoNS breakpoints, CA values were 85% and 75%, respectively, and there were 72% and 64% VMEs, respectively, and 0 MEs. For cefoxitin MICs interpreted using breakpoints, CA was 81%, and there were 60% VMEs and no MEs. Our data demonstrate that oxacillin DD or MIC testing methods using the current breakpoints reliably identify -mediated oxacillin resistance in, while cefoxitin DD and MIC testing methods perform poorly.

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“…They typically cause otitis or pyoderma in dogs, and rarely may be isolated from cats or birds (Abraham et al, 2007; Briscoe et al, 2009; Cain, 2013). Prevalence from skin, nares, mouth, or perineal carriage in the absence of disease is low, typically ≤2%, but is higher among diseased pets (Abraham et al, 2007; Griffeth et al, 2008; Beck et al, 2012; May et al, 2012). Of concern is the propensity for clinical isolates to be methicillin resistant, with many veterinary studies in the last decade reporting rates of 50% or higher (Kania et al, 2004; Bemis et al, 2006; Vanni et al, 2009; Cain et al, 2011a,b; Penna et al, 2013).…”

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confidence: 99%

Two coagulase-negative staphylococci emerging as potential zoonotic pathogens: wolves in sheep's clothing?

et al. 2013

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Nasal carriage of Staphylococcus schleiferi from healthy dogs and dogs with otitis, pyoderma or both

Cited by 26 publications

References 27 publications

Strain typing and antimicrobial susceptibility of methicillin-resistant coagulase-positive staphylococcal species in dogs and people associated with dogs in Thailand

Strain typing and antimicrobial susceptibility of methicillin-resistant coagulase-positive staphylococcal species in dogs and people associated with dogs in Thailand

Evaluation of Oxacillin and Cefoxitin Disk Diffusion and MIC Breakpoints Established by the Clinical and Laboratory Standards Institute for Detection of mecA -Mediated Oxacillin Resistance in Staphylococcus schleiferi

Two coagulase-negative staphylococci emerging as potential zoonotic pathogens: wolves in sheep's clothing?

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