Nasal colonization and antibiotic resistance patterns of &lt;i&gt;Staphylococcus&lt;/i&gt; species isolated from healthy horses in Tripoli, Libya

Othman, Aesha A; Hiblu, Murad A; Abbassi, Mohamed Salah; Abouzeed, Yousef M.; Ahmed, Mohamed O.

doi:10.1294/jes.32.61

Cited by 11 publications

(9 citation statements)

References 23 publications

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“…S. sciuri was the most prevalent species among donkeys. S. sciuri and S. lentus were also among the most frequently detected CoNS species in horses [47][48][49][50][51]. However, other studies report other species of CoNS as the most common in horses such as S. capitis and S. equorum [14,52].…”

Section: Discussionmentioning

confidence: 99%

A One Health Approach Molecular Analysis of Staphylococcus aureus Reveals Distinct Lineages in Isolates from Miranda Donkeys (Equus asinus) and Their Handlers

et al. 2022

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Donkeys (Equus asinus) are in decline in Europe. Occupational exposure to farm animals has been associated with increased staphylococci carriage. We aimed to isolate S. aureus and coagulase-negative staphylococci (CoNS) from donkeys and handlers and characterize the antimicrobial resistance profiles and genetic lineages of S. aureus strains. Oral and nasal swab samples were collected from 49 Miranda donkeys and 23 handlers from 15 different farms. Staphylococci species were identified by MALDI-TOF MS. The presence of antimicrobial resistance genes and virulence factors was investigated by PCR. Molecular typing was performed in S. aureus isolates. From the 49 donkey samples, 4 S. aureus (8.2%) and 21 CoNS (42.9%) were isolated. Ten handlers (43.5%) were carriers of S. aureus and 4 (17.4%) carried CoNS. The CoNS isolates showed resistance to several classes of antimicrobials encoded by the mecA, aph (3′)-IIIa, ant (4′)-Ia, tetM, tetK, lnuA, ermB, ermC, dfrA and dfrG genes. S. aureus isolates were resistant to penicillin, aminoglicosides and tetracycline harboring the blaZ, aph (3′)-IIIa, tetL, tetM and tetK genes. All S. aureus isolates from donkeys belonged to ST49 and spa-type t208 while the strains isolated from the handlers were ascribed to 3 STs and 7 spa-types. However, human isolates were from different STs than the donkey isolates. Donkeys are mainly colonized by methicillin-resistant S. sciuri. S. aureus transmission between donkeys and their handlers appears not to have occurred since the isolates belonged to different genetic lineages.

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Section: Discussionmentioning

confidence: 99%

A One Health Approach Molecular Analysis of Staphylococcus aureus Reveals Distinct Lineages in Isolates from Miranda Donkeys (Equus asinus) and Their Handlers

et al. 2022

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“…The most common sites for MRSA colonization in humans are the nasal cavity, hair, nails, skin, vaginal axilla, and perineum. In contrast, the most common sites for MRSA colonization in horses are the nasal cavity [57].…”

Section: La-mrsa In Horsementioning

confidence: 98%

“…LA-MRSA infection and colonization is a serious condition that can occur in horses as well as in humans. The risk factors reported in cases of MRSA infection and colonization in humans were history of antibiotic administration, contact with hospital staff, history of previous hospitalization, and hospital overcrowding [57,[98][99][100][101]. Meanwhile, the reported risk factors for LA-MRSA infection and colonization in horses have not been adequately evaluated [102].…”

Section: Risk Factor For Spreading La-mrsa In Horsementioning

confidence: 99%

A review of horses as a source of spreading livestock-associated methicillin-resistant Staphylococcus aureus to human health

et al. 2022

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Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) was first discovered in horses in 1989. Since then, LA-MRSA has begun to be considered an important strain of pathogenic bacteria in horses, which can cause LA-MRSA infection and colonization in humans with public health impacts. The anterior nares are the primary site of LA-MRSA colonization in horses, although LA-MRSA colonization may also occur in the gastrointestinal tract in horses. LA-MRSA-infected horses typically exhibit clinical infection or may not exhibit clinical infection. There are two potential risks associated with LA-MRSA colonization in horses: The possibility of disease development in horses infected with LA-MRSA and the possibility of LA-MRSA transfer to humans and other horses. The diagnosis of LA-MRSA in horses can be made by conducting in vitro sensitivity testing for oxacillin and cefoxitin, and then followed by a molecular test using polymerase chain reaction. LA-MRSA transmission in animal hospitals and on farms is most likely due to contact with horses infected or colonized by LA-MRSA. The history of prior antibiotic administration, history of prior LA-MRSA colonization, and length of equine hospitalization were described as risk factors in cases of infection and colonization of LA-MRSA in horses. Nebulized antibiotics may be a viable alternative to use in horses, but nebulized antibiotics are only used in horses that are persistently colonized with LA-MRSA. Controlling the spread of LA-MRSA in horses can be done by regularly washing horses, eradicating vectors in horse stalls such as rats, and maintaining the cleanliness of the stable and animal hospital environment. Meanwhile, cleaning hands, using gloves, and donning protective clothes are ways that humans can prevent the transmission of LA-MRSA when handling horses. This review will explain the definition of LA-MRSA in general, LA-MRSA in horses, the epidemiology of LA-MRSA in horses, the diagnosis of LA-MRSA in horses, the transmission of LA-MRSA in horses, risk factors for spreading LA-MRSA in horses, public health impact, treatment of LA-MRSA infection in horses, and control of the spread of LA-MRSA in horses.

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“…Methyloversatilis is capable of reducing NO 3 − or NO 2 − , and capable of C 1 -assimilation via the serine cycle and Calvin cycle (Anthony, 1982;Lu et al, 2014). Most of Staphylococcus is non-pathogenic parasitic bacteria, and some can cause infectious diseases in poultry (Othman et al, 2021). Hymenobacter is a radiation-resistant bacterium commonly found in rhizosphere soil and phyllosphere (Park et al, 2022;Stevens et al, 2021).…”

Section: Bacterial Function Analysis (Generic Level)mentioning

confidence: 99%

Community composition and differential analysis of rhizosphere soil microorganism and endophytes in Schisandra sphenanthera Rehd. et Wils.

Wang¹,

Qin²,

Han³

et al. 2022

Preprint

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Background and aims: Endophyte and rhizosphere soil microorganism are vital microbial environments of the plant, namely plant microenvironments. A robust understanding of the structural composition of the microbiome present in below-ground and above-ground communities has remained elusive. Schisandra sphenanthera Rehd. et Wils. is a kind of traditional Chinese Medicine (TCM) of Schisandra, which can protect the kidney and liver. Methods: In this study, high-throughput sequencing analysis is applied to unravel microbial communities in rhizosphere soil and different parts of wild S. sphenanthera, and the movement regularity of endophytes in plant tissues.Results: There are differences in microbial composition and diversity between rhizosphere soil and four parts of S. sphenanthera. Proteobacteria, Cyanobacteria, and Acidobacteria are main bacteria, Ascomycota and Basidiomycota are main fungi at phylum level of microbe in S. sphenanthera. There are 12 common bacterial genera and 11 common fungal genera in rhizosphere soil and different parts of S. sphenanthera. In addition, each of the four parts and rhizosphere soil have its own dominant communities, such as Achromobacter (stem and leaf) and Methylobacterium (leaf). OTUs clustering results indicate that the bacterial community of root is greatly influenced by rhizosphere soil, while the microbial community of stem and fruit are greatly affected by the microorganisms of leaf. Conclusions: Understanding the microbial community structure and diversity in rhizosphere soil and different parts of S. sphenanthera can provide basis for further study of host-microbial interactions of S. sphenanthera in phytoremediation, sustainable utilization, and secondary metabolite production.

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Nasal colonization and antibiotic resistance patterns of <i>Staphylococcus</i> species isolated from healthy horses in Tripoli, Libya

Cited by 11 publications

References 23 publications

A One Health Approach Molecular Analysis of Staphylococcus aureus Reveals Distinct Lineages in Isolates from Miranda Donkeys (Equus asinus) and Their Handlers

A One Health Approach Molecular Analysis of Staphylococcus aureus Reveals Distinct Lineages in Isolates from Miranda Donkeys (Equus asinus) and Their Handlers

A review of horses as a source of spreading livestock-associated methicillin-resistant Staphylococcus aureus to human health

Community composition and differential analysis of rhizosphere soil microorganism and endophytes in Schisandra sphenanthera Rehd. et Wils.

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