Nasal Nitric Oxide and Ciliary Videomicroscopy: Tests Used for Diagnosing Primary Ciliary Dyskinesia

Shapiro, Adam J.; Chilvers, Mark; Davis, Stephanie D.; Leigh, Margaret W.

doi:10.1007/978-1-4939-1801-0_4

Cited by 2 publications

(11 citation statements)

References 60 publications

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“…The brush is moved posteriorly and anteriorly several times and then withdrawn. Epithelial strips obtained are dislodged by agitating the brush in cell culture medium …”

Section: Respiratory Ciliated Epithelium Sample Collectionmentioning

confidence: 99%

“…The relationship between CBP and temperature has not been as extensively studied. Due to the rapid CBF, interpretation of beat pattern with DHSV can be difficult at 37°C, even with frame‐by‐frame analysis . It was suggested that at low temperatures, cilia have an abnormal CBP or are immotile .…”

Section: Environmental Conditions For Ciliary Functional Analysismentioning

confidence: 99%

“…At standard video recording rates (25‐30 Hz), artifacts due to signal aliasing prevent measurements of CBF more than 15 Hz and prevent CBP analysis; this can be avoided by using higher recording rates . While CBF up to 25 Hz may be analyzed by recording at frame rate of 50 Hz, visualization of the complete beat cycle requires recording at frame rate of 200 Hz to visualize at least four frames throughout the beat cycle; but frame rate up to 400 Hz is required to allow a precise evaluation of the beat pattern …”

Section: Respiratory Ciliated Samples Preparationmentioning

confidence: 99%

“…A lack of standardization and precise operating procedures for processing and analyzing samples using DHSV …”

Section: Introductionmentioning

confidence: 99%

“…DHSV abnormalities may be very subtle and require extensive training and experience, remaining strongly dependant on the experience of the investigator …”

Section: Introductionmentioning

confidence: 99%

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Ciliary functional analysis: Beating a path towards standardization

Kempeneers¹,

Seaton

Espinosa

et al. 2019

Pediatric Pulmonology

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Primary ciliary dyskinesia is an inherited disorder in which respiratory cilia are stationary, or beat in a slow or dyskinetic manner, leading to impaired mucociliary clearance and significant sinopulmonary disease. One diagnostic test is ciliary functional analysis using digital high‐speed video microscopy (DHSV), which allows real‐time analysis of complete ciliary function, comprising ciliary beat frequency (CBF) and ciliary beat pattern (CBP). However, DHSV lacks standardization. In this paper, the current knowledge of DHSV ciliary functional analysis is presented, and recommendations given for a standardized protocol for ciliary sample collection and processing. A proposal is presented for a quantitative and qualitative CBP evaluation system, to be used to develop international consensus agreement, and future DHSV research areas are identified.

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“…The brush is moved posteriorly and anteriorly several times and then withdrawn. Epithelial strips obtained are dislodged by agitating the brush in cell culture medium …”

Section: Respiratory Ciliated Epithelium Sample Collectionmentioning

confidence: 99%

Section: Environmental Conditions For Ciliary Functional Analysismentioning

confidence: 99%

Section: Respiratory Ciliated Samples Preparationmentioning

confidence: 99%

“…A lack of standardization and precise operating procedures for processing and analyzing samples using DHSV …”

Section: Introductionmentioning

confidence: 99%

“…DHSV abnormalities may be very subtle and require extensive training and experience, remaining strongly dependant on the experience of the investigator …”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Ciliary functional analysis: Beating a path towards standardization

Kempeneers¹,

Seaton

Espinosa

et al. 2019

Pediatric Pulmonology

Self Cite

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Nasal Nitric Oxide Levels in HIV Infection: A Cross-Sectional Study

Costiniuk

Mehraj

Routy

et al. 2018

AIDS Research and Treatment

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Introduction Low levels of nasal NO have been associated with increased propensity to rhinosinusitis and respiratory tract infections. Our objective was to describe nasal NO levels in HIV-infected individuals versus healthy controls and determine possible risk factors for reduced nasal NO levels. Materials and Methods HIV-infected individuals and healthy controls were recruited. Participants underwent nasal NO testing by standardized methods using a CLD88 chemiluminescence analyzer and completed the Sinonasal Outcome Test-20 (SNOT-20) on symptoms of rhinosinusitis. Results Participants included 41 HIV-infected individuals with suppressed VL on antiretroviral therapy (ART group), 5 HIV-infected individuals with detectable VL off ART (viremic group), and 12 healthy controls (HC group). Mean nasal NO level was 253 (±77) nL/min in the ART group, 213 (±48) nL/min in the viremic group, and 289 (±68) nL/min in the HC group (p = 0.133; ANOVA). There was no correlation between nasal NO level and VL in viremic individuals (r = −0.200; p = 0.747). Differences were observed in mean total points on the SNOT-20 which were 19 (±16)/100, 18 (±26)/100, and 4 (±4)/100 in the ART, viremic, and HC groups, respectively (p = 0.013; ANOVA). Conclusion Healthy individuals, HIV patients on ART, and viremic individuals off ART display similar nasal NO levels. However, rhinosinusitis symptoms remain prominent despite ART-treatment.

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Nasal Nitric Oxide and Ciliary Videomicroscopy: Tests Used for Diagnosing Primary Ciliary Dyskinesia

Cited by 2 publications

References 60 publications

Ciliary functional analysis: Beating a path towards standardization

Ciliary functional analysis: Beating a path towards standardization

Nasal Nitric Oxide Levels in HIV Infection: A Cross-Sectional Study

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