Nascent proteomes of ischemic-injured and ischemic-tolerant neuronal cells

Zhou, An; Simon, Roger P.; David, Larry L.

doi:10.1504/ijcbdd.2011.038656

Cited by 7 publications

(7 citation statements)

References 12 publications

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“…After incubation, proteins were extracted from individual PBMC preparations and pooled according to study groups as follows: female patients, male patients, female controls, and male controls. The AHA-labeled PBMC proteins (i.e., nascent proteome) were isolated from the total proteome by means of Click reaction 3, 4 as described in SMM.…”

Section: Study Subjects and Methodsmentioning

confidence: 99%

Nascent Proteomes in Peripheral Blood Mononuclear Cells as a Novel Source for Biomarker Discovery in Human Stroke

Bian

Simon

et al. 2014

Stroke

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Background and Purpose The proteome of newly synthesized proteins (nascent proteome) in peripheral blood mononuclear cells (PBMC) can be a novel source of stroke biomarkers. Changes in the PBMC nascent proteome after stroke reflect the dynamic response-in-action not detectable in the total proteome (all existing proteins) in blood. Here we test the application of nascent proteomics as a novel approach for stroke biomarker discovery. Methods The PBMC nascent proteome in human blood was determined by metabolic labeling of fresh PBMC cultures with azidohomoalanine (AHA, an azide-containing methionine surrogate), followed by mass spectrometry detection and quantitation of AHA-labeled proteins. The PBMC nascent and total proteomes were compared between stroke patients and matched controls. Results Both PBMC nascent and total proteomes showed differences between stroke patients and controls. Results of hierarchical clustering analysis of proteomic data revealed greater changes in the nascent than in the total PBMC proteomes, supporting the usefulness of the PBMC nascent proteome as a novel source of stroke biomarkers. Conclusions Nascent proteomes in PBMC can be a novel source for biomarker discovery in human stroke.

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Section: Study Subjects and Methodsmentioning

confidence: 99%

Nascent Proteomes in Peripheral Blood Mononuclear Cells as a Novel Source for Biomarker Discovery in Human Stroke

Bian

Simon

et al. 2014

Stroke

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“…In 2011, we published the first study on nascent proteomes of cultured, ischemic neuronal cells 35. The results support the feasibility.…”

Section: Nascent Proteomics In Stroke Researchmentioning

confidence: 67%

Proteomics in Stroke Research: Potentials of the Nascent Proteomics

Zhou¹

2016

Journal of Investigative Medicine

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Among omics, the proteomics assumes a unique role in that it offers the effectors or actuators of a biological condition. This brief review attempts to summarize the development in a relatively new but important subdiscipline of proteomics, the so-called nascent proteomics, and its potential applications in stroke research. First, we will discuss a few examples of proteomics-led discoveries in stroke research, and challenges or unmet demands when using commonly practiced proteomics approaches. Then we will introduce nascent proteomics and its studying tools, followed by discussions on its potentials in stroke research.

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“…To get insight into pathophysiological changes in the ischemic penumbra, it is desirable to investigate its translational changes. With quantitative proteomic analysis, we detected 3,837 proteins qualified with high confidence ( Supplementary Table S5), which is much larger than previous cell-based studies in which 524-1081 proteins were identified under OGD treatments (Jin et al, 2004;Datta et al, 2009;Zhou et al, 2011;Herrmann et al, 2013). Importantly, 427 and 398 proteins in our study had their abundance changed ≥1.4-fold (p < 0.05) between H−G or H+LG and N+G treatments, respectively, and 105 proteins had their abundance difference ≥1.5-fold between H−G and H+LG treated cells.…”

Section: Discussionmentioning

confidence: 77%

Quantitative Proteomics Reveals the Beneficial Effects of Low Glucose on Neuronal Cell Survival in an in vitro Ischemic Penumbral Model

Kittur

Hung

et al. 2020

Front. Cell. Neurosci.

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Understanding proteomic changes in the ischemic penumbra are crucial to rescue those salvageable cells and reduce the damage of an ischemic stroke. Since the penumbra region is dynamic with heterogeneous cells/tissues, tissue sampling from animal models of stroke for the molecular study is a challenge. In this study, cultured hippocampal HT22 cells under hypoxia treatment for 17.5 h with 0.69 mM low glucose (H+LG) could mimic ischemic penumbral cells since they had much higher cell viability and viable cell number compared to hypoxia without glucose (H−G) treatment. To validate established cell-based ischemic penumbral model and understand the beneficial effects of low glucose (LG), quantitative proteomics analysis was performed on H+LG, H−G, and normoxia with normal 22 mM glucose (N+G) treated cells. We identified 427 differentially abundant proteins (DAPs) between H−G and N+G and further identified 105 DAPs between H+LG and H−G. Analysis of 105 DAPs revealed that LG promotes cell survival by activating HIF1α to enhance glycolysis; preventing the dysregulations of extracellular matrix remodeling, cell cycle and division, and antioxidant and detoxification; as well as attenuating inflammatory reaction response, protein synthesis and neurotransmission activity. Our results demonstrated that this established cell-based system could mimic penumbral conditions and can be used for molecular studies.

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Nascent proteomes of ischemic-injured and ischemic-tolerant neuronal cells

Cited by 7 publications

References 12 publications

Nascent Proteomes in Peripheral Blood Mononuclear Cells as a Novel Source for Biomarker Discovery in Human Stroke

Nascent Proteomes in Peripheral Blood Mononuclear Cells as a Novel Source for Biomarker Discovery in Human Stroke

Proteomics in Stroke Research: Potentials of the Nascent Proteomics

Quantitative Proteomics Reveals the Beneficial Effects of Low Glucose on Neuronal Cell Survival in an in vitro Ischemic Penumbral Model

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