Natalamycin A, an ansamycin from a termite-associated Streptomyces sp.

Kim, Ki‐Hyun; Ramadhar, Timothy R.; Beemelmanns, Christine; Cao, Shugeng; Poulsen, Michael; Currie, Cameron R.; Clardy, Jon

doi:10.1039/c4sc01136h

Cited by 92 publications

(92 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These studies led to selective antifungal agents such as the depsipeptide dentigerumycin from a fungus-growing ant ( Apterostigma dentigerum ) symbiont, 2 and the polyene peroxide mycangimycin from a Southern pine beetle ( Dendroctonus frontalis ) symbiont. 3 While our initial studies of bacteria associated with a species of fungus-growing termites ( Macrotermes natalensis ) 4 led to the discovery of chemically-intriguing compounds like the microtermolides 5 and natalamycin A, 6 they did not identify any selective antifungal agents. We focused our efforts to find selective agents from bacteria associated with M. natalensis through a systematic combination of LC-HRMS-based dereplication and bioactivity assays against the basidiomycete cultivar ( Termitomyces spp.)…”

mentioning

confidence: 99%

Macrotermycins A–D, Glycosylated Macrolactams from a Termite-Associated Amycolatopsis sp. M39

et al. 2017

Self Cite

View full text Add to dashboard Cite

Bioassay-guided metabolomic analyses led to the characterization of four new 20-membered glycosylated polyketide macrolactams – macrotermycins A-D – from a termite-associated actinomycete, Amycolatopsis sp. M39. M39’s sequenced genome revealed the macrotermycin’s putative biosynthetic gene cluster. Macrotermycins A and C had antibacterial activity against human-pathogenic S. aureus and of greater ecological relevance, they also had selective antifungal activity against a fungal parasite of the termite fungal garden.

show abstract

mentioning

confidence: 99%

Macrotermycins A–D, Glycosylated Macrolactams from a Termite-Associated Amycolatopsis sp. M39

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…As the most prolific secondary metabolite producers, actinomycetes account for approximately 41% of the bioactive microbial metabolites that have been discovered (1). In recent years, with the emergence of antibiotic-resistant microbial pathogens and rediscovery of known bioactive compounds, special habitats in the ocean (2,3), sponges (4), plants (5,6), and insects (7) have been found to be potentially important in the search and discovery of bioactive compound-producing strains. Nevertheless, soil is still the predominant source of secondary metabolite-producing actinomycetes.…”

mentioning

confidence: 99%

Red Soils Harbor Diverse Culturable Actinomycetes That Are Promising Sources of Novel Secondary Metabolites

Guo

Ning

et al. 2015

Appl Environ Microbiol

View full text Add to dashboard Cite

Red soils, which are widely distributed in tropical and subtropical regions of southern China, are characterized by low organic carbon, high content of iron oxides, and acidity and, hence, are likely to be ideal habitats for acidophilic actinomycetes. However, the diversity and biosynthetic potential of actinomycetes in such habitats are underexplored. Here, a total of 600 actinomycete strains were isolated from red soils collected in Jiangxi Province in southeast China. 16S rRNA gene sequence analysis revealed a high diversity of the isolates, which were distributed into 26 genera, 10 families, and 7 orders within the class Actinobacteria; these taxa contained at least 49 phylotypes that are likely to represent new species within 15 genera. The isolates showed good physiological potentials for biosynthesis and biocontrol. Chemical screening of 107 semirandomly selected isolates spanning 20 genera revealed the presence of at least 193 secondary metabolites from 52 isolates, of which 125 compounds from 39 isolates of 12 genera were putatively novel. Macrolides, polyethers, diketopiperazines, and siderophores accounted for most of the known compounds. The structures of six novel compounds were elucidated, two of which had a unique skeleton and represented characteristic secondary metabolites of a putative novel Streptomyces phylotype. These results demonstrate that red soils are rich reservoirs for diverse culturable actinomycetes, notably members of the families Streptomycetaceae, Pseudonocardiaceae, and Streptosporangiaceae, with the capacity to synthesize novel bioactive compounds. While high-throughput sequencing methods expand our understanding of species diversity in microbial communities and reveal the presence of many new groups that were previously undetected in cultivation studies, the availability of pure cultures is still essential in order to discover new or useful bioactive compounds from microorganisms. As the most prolific secondary metabolite producers, actinomycetes account for approximately 41% of the bioactive microbial metabolites that have been discovered (1). In recent years, with the emergence of antibiotic-resistant microbial pathogens and rediscovery of known bioactive compounds, special habitats in the ocean (2, 3), sponges (4), plants (5, 6), and insects (7) have been found to be potentially important in the search and discovery of bioactive compound-producing strains. Nevertheless, soil is still the predominant source of secondary metabolite-producing actinomycetes. Unexplored and underexplored soil habitats have been found to be a rich source of actinomycetes that produce novel bioactive metabolites (8-10). Acidic soils in China, mostly red soils, are one such habitat.Red soils are generated by abundant rainfall and high temperature and are widespread in tropical and subtropical regions of southern China, covering about 1.13 million km 2 (11). These soils have been subjected to intensive weathering, and mineral nutrients (including Ca, Mg, P, and K) in the soil are mostly eluted, form...

show abstract

“…residing in the gut of the termite Odontotermes formosanus (Bi et al 2011), and polyketide synthase-nonribosomal peptide synthetase hybrid natural products, macrotermolides A (16) have been discovered from a termite-associated Streptomyces sp. (Carr et al 2012) Furthermore, continuous research on the secondary metabolites of termite-associated actinomycetes from Macrotermes natalensis colonies led to the isolation of a highly modified geldanamycin compound, natalamycin A (17) (Kim et al 2014), and the antifungal compounds, tyroscherin and N-methyltyroscherin (18), were identified in a Pseudallescheria boydii fungal strain isolated from the termite Nasutitermes sp. in France (Nirma et al 2013).…”

Section: Chemical Biology Of Gut Bacteria and Their Secondary Metabolmentioning

confidence: 99%

Considerations of the chemical biology of microbial natural products provide an effective drug discovery strategy

Choi

2015

Arch. Pharm. Res.

View full text Add to dashboard Cite

Conventional approaches to natural product drug discovery rely mainly on random searches for bioactive compounds using bioassays. These traditional approaches do not incorporate a chemical biology perspective. Searching for bioactive molecules using a chemical and biological rationale constitutes a powerful search paradigm. Here, the authors review recent examples of the discovery of bioactive natural products based on chemical and biological interactions between hosts and symbionts, and propose this method provides a more effective means of exploring natural chemical diversity and eventually of discovering new drugs.

show abstract

Natalamycin A, an ansamycin from a termite-associated Streptomyces sp.

Cited by 92 publications

References 44 publications

Macrotermycins A–D, Glycosylated Macrolactams from a Termite-Associated Amycolatopsis sp. M39

Macrotermycins A–D, Glycosylated Macrolactams from a Termite-Associated Amycolatopsis sp. M39

Red Soils Harbor Diverse Culturable Actinomycetes That Are Promising Sources of Novel Secondary Metabolites

Considerations of the chemical biology of microbial natural products provide an effective drug discovery strategy

Contact Info

Product

Resources

About