Natamycin-loaded electrospun poly(ε-caprolactone) nanofibers as an innovative platform for antifungal applications

Veras, Flávio Fonseca; Ritter, Ana Carolina; Roggia, Isabel; Pranke, Patrícia; Pereira, Cláudio Nunes; Brandelli, Adriano

doi:10.1007/s42452-020-2912-z

Cited by 17 publications

(14 citation statements)

References 66 publications

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“…Until now, polymers other than PA, for example, PCL ( Veras et al, 2020 ) or polyvinyl alcohol ( Göksen et al, 2021 ), have been used for functionalized materials with NAT, RE, and GTE. For example, Göksen et al (2021) verified the antimicrobial effect of polyvinyl alcohol/RE nanofibers directly on food samples.…”

Section: Resultsmentioning

confidence: 99%

“…Until now, a variety of promising active food packaging from nanofibers functionalized with other substances was developed ( Duan et al, 2021 ; Göksen et al, 2021 ). In terms of microbiological safety and better benefits of these materials, usually only antimicrobial activity on model organisms or food samples is monitored ( Veras et al, 2020 ; Göksen et al, 2021 ), whereas their ability to suppress biofilm formation and retain microbial cells is studied rarely. According to our knowledge, only one study focusing on all the mentioned aspects—antimicrobial, antibiofilm, and microbial barrier properties—of materials potentially applicable as food packaging has been published, but the tests were not further performed on food samples ( Babu et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

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Potential of Polyamide Nanofibers With Natamycin, Rosemary Extract, and Green Tea Extract in Active Food Packaging Development: Interactions With Food Pathogens and Assessment of Microbial Risks Elimination

Lencová

Stiborová

Munzarová³

et al. 2022

Front. Microbiol.

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Increasing microbial safety and prolonging the shelf life of products is one of the major challenges in the food industry. Active food packaging made from nanofibrous materials enhanced with antimicrobial substances is considered a promising way. In this study, electrospun polyamide (PA) nanofibrous materials functionalized with 2.0 wt% natamycin (NAT), rosemary extract (RE), and green tea extract (GTE), respectively, were prepared as active packaging and tested for the food pathogens Escherichia coli, Listeria monocytogenes, Salmonella enterica, and Staphylococcus aureus. The PAs exhibited: (i) complete retention of bacterial cells reaching 6.0–6.4 log10removal, (ii) antimicrobial activity with 1.6–3.0 log10suppression, and (iii) antibiofilm activity with 1.7–3.0 log10suppression. The PAs prolonged the shelf life of chicken breast; up to 1.9 log10(CFU/g) suppression of total viable colonies and 2.1 log10(CFU/g) suppression of L. monocytogenes were observed after 7 days of storage at 7°C. A beneficial effect on pH and sensory quality was verified. The results confirm microbiological safety and benefits of PA/NAT, PA/RE, and PA/GTE and their potential in developing functional and ecological packaging.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Potential of Polyamide Nanofibers With Natamycin, Rosemary Extract, and Green Tea Extract in Active Food Packaging Development: Interactions With Food Pathogens and Assessment of Microbial Risks Elimination

Lencová

Stiborová

Munzarová³

et al. 2022

Front. Microbiol.

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“…It is usually applied in the mid-IR range from 25,000–2500 nm (400–4000 cm −1 ). Here, it is possible to obtain a good overview of functional groups in single compounds and in mixtures, which is impossible to achieve with simple elemental analysis [ 24 , 25 , 42 , 94 , 98 ]. IR spectroscopy always requires choosing a suitable background sample, which is normally air or the pure solid substrate on which the fiber is deposited.…”

Section: Characterizationmentioning

confidence: 99%

“…Moving to smaller wavelengths, near-infrared (NIR) spectroscopy in the wavelength range of 800–2000 nm (12,500–5000 cm −1 ) is playing an ever-larger role in pharmacology [ 97 , 98 ]. Here, vibrational overtones give rise to very complex spectra, which are mainly employed for fingerprinting, i.e., the correlated presence of a plethora of absorption signals is interpreted as the presence of a compound.…”

Section: Characterizationmentioning

confidence: 99%

Peptide-Based Electrospun Fibers: Current Status and Emerging Developments

et al. 2021

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Electrospinning is a well-known, straightforward, and versatile technique, widely used for the preparation of fibers by electrifying a polymer solution. However, a high molecular weight is not essential for obtaining uniform electrospun fibers; in fact, the primary criterion to succeed is the presence of sufficient intermolecular interactions, which function similar to chain entanglements. Some small molecules able to self-assemble have been electrospun from solution into fibers and, among them, peptides containing both natural and non-natural amino acids are of particular relevance. Nowadays, the use of peptides for this purpose is at an early stage, but it is gaining more and more interest, and we are now witnessing the transition from basic research towards applications. Considering the novelty in the relevant processing, the aim of this review is to analyze the state of the art from the early 2000s on. Moreover, advantages and drawbacks in using peptides as the main or sole component for generating electrospun nanofibers will be discussed. Characterization techniques that are specifically targeted to the produced peptide fibers are presented.

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“…Natamycin can bind to ergosterol within the fungal plasma membrane, leading to a loss of membrane function [ 13 ]. While Natamycin is widely used as a food preservative, some factors can compromise its application effectiveness on the food surface, such as poor water solubility, inactivation by food matrix components, and rapid migration [ 14 ]. Natamycin’s low aqueous dissolvability restricts its availability for antimicrobial activity in the molecular state and its diffusion rate to the place of antifungal action.…”

Section: Introductionmentioning

confidence: 99%

Packaging fortified with Natamycin nanoparticles for hindering the growth of toxigenic Aspergillus flavus and aflatoxin production in Romy cheese

Fayed¹,

ELsayed²,

Ali³

2021

J Adv Vet Anim Res

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Objective: This study assessed the effect of cellulose sheets fortified with Natamycin-loaded alginate nanoparticles on the growth of toxigenic Aspergillus flavus and aflatoxin production on the superficial layer of Egyptian Romy cheese after 12 weeks of maturation. Materials and Methods: Toxigenic A. flavus (GenBank accession No. MT645073) was inoculated into the outer surface of Egyptian Romy cheese (at 5 log CFU/gm) and wrapped with a cellulose sheet fortified with Natamycin-loaded alginate nanoparticles. Unwrapped control contaminated Romy wheels were made as well as non-contaminated wrapped cheese wheels for sensory evaluation. Romy cheese wheels were stored at a temperature similar to commercial methods for 12 weeks. Fungal counts were enumerated during this time, and enzyme-linked immune sorbent assay detected aflatoxin after the 4th week of maturation storage. Results: In cheese samples covered with cellulose sheets containing Natamycin-loaded alginate nanoparticles, the fungal count was reduced by 2 log approximately in contrast to control samples after the 2nd week of storage. However, within the 8th week of storage, the greatest significant reduction ( p < 0.05) was seen where fungal growth was hindered entirely to the end of the ripening period. The mean values for taste, color, flavor, and overall acceptability were 4, 4.7, 4.09, and 4.3, respectively. Furthermore, in the treated samples, the total aflatoxin concentration was decreased by 78.6% relative to the untreated control one. Conclusion: Using cellulose sheets fortified with Natamycin-loaded alginate nanoparticles in Egyptian Romy cheese wrapping could be an effective way of controlling A. flavus and subsequent aflatoxin production without influencing the typical taste, color, flavor, and overall appearance of traditional Romy cheese.

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Natamycin-loaded electrospun poly(ε-caprolactone) nanofibers as an innovative platform for antifungal applications

Cited by 17 publications

References 66 publications

Potential of Polyamide Nanofibers With Natamycin, Rosemary Extract, and Green Tea Extract in Active Food Packaging Development: Interactions With Food Pathogens and Assessment of Microbial Risks Elimination

Potential of Polyamide Nanofibers With Natamycin, Rosemary Extract, and Green Tea Extract in Active Food Packaging Development: Interactions With Food Pathogens and Assessment of Microbial Risks Elimination

Peptide-Based Electrospun Fibers: Current Status and Emerging Developments

Packaging fortified with Natamycin nanoparticles for hindering the growth of toxigenic Aspergillus flavus and aflatoxin production in Romy cheese

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