NatB-Mediated N-Terminal Acetylation Affects Growth and Biotic Stress Responses

Huber, Monika; Bienvenut, Willy V.; Linster, Eric; Stephan, Iwona; Armbruster, Laura; Sticht, Carsten; Layer, Dominik; Lapouge, Karine; Meinnel, Thierry; Sinning, Irmgard; Giglione, Carmela; Hell, Ruediger; Wirtz, Markus

doi:10.1104/pp.19.00792

Cited by 53 publications

(80 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gene expression analysis confirmed that knockdown of NAA50 results in an up-regulation of defense signaling. These results are consistent with the observation that loss of NAA50 results in an accumulation of stress response proteins (Armbruster et al, 2020).…”

Section: Naa50 Is Required For Growth and The Suppression Of Stress Rsupporting

confidence: 92%

“…To investigate the role of NAA50 in plants, we characterized two T-DNA insertion mutants (SAIL_1210_A02 and SAIL_1186_A03), which we designated naa50-1 and naa50-2, respectively. The naa50-1 mutant contains a T-DNA insertion in intron 2, while naa50-2 contains a T-DNA insertion in intron 1, and neither produces detectable full-length transcript or NAA50 protein (Armbruster et al, 2020). We confirmed the presence of homozygous T-DNA insertions in NAA50 in each line using PCR-based genotyping (see "Materials and Methods").…”

Section: Arabidopsis Naa50 Is Highly Conserved and Essential For Devementioning

confidence: 72%

“…Our understanding of how NATs function comes primarily from work in human cell culture and yeast. However, recent work in plants has demonstrated a role for NTAs in regulating diverse processes (Linster et al, 2015;Xu et al, 2015;Huber et al, 2020). Posttranslational modification of proteins has long been appreciated as a mechanism by which cell signaling and crosstalk is regulated (Hunter, 2007).…”

Section: Naa50 Is Required For Growth and The Suppression Of Stress Rmentioning

confidence: 99%

“…Reduced expression of NAA10 and NAA15 alters root morphology, enhances the growth of the primary root and inhibits the growth of lateral roots (Linster et al, 2015). NatB also appears to be required for development, as partial loss of NatB components NAA20 and NAA25 result in dwarfism (Ferrández-Ayela et al, 2013;Xu et al, 2015;Huber et al, 2020). Loss of NAA30, the catalytic component of NatC, results in minor dwarfism as well as defects in photosystem II efficiency (Pesaresi et al, 2003).…”

Section: Naa50 Is Required For Growth and The Suppression Of Stress Rmentioning

confidence: 99%

See 3 more Smart Citations

Loss of the Acetyltransferase NAA50 Induces Endoplasmic Reticulum Stress and Immune Responses and Suppresses Growth

Neubauer

Innes

2020

Plant Physiol.

View full text Add to dashboard Cite

Stress signaling in plants is carefully regulated to ensure proper development and reproductive fitness. Overactive defense signaling can result in dwarfism as well as developmental defects. In addition to requiring a substantial amount of energy, plant stress responses place a burden upon the cellular machinery, which can result in the accumulation of misfolded proteins and endoplasmic reticulum (ER) stress. Negative regulators of stress signaling, such as ENHANCED DISEASE RESISTANCE 1 (EDR1), ensure that stress responses are properly suspended when they are not needed, thereby conserving energy for growth and development. Here, we describe the role of an uncharacterized N-terminal acetyltransferase, NAA50, in the regulation of plant development and stress responses in Arabidopsis (Arabidopsis thaliana). Our results demonstrate that NAA50, an interactor of EDR1, plays an important role in regulating the tradeoff between plant growth and defense. Plants lacking NAA50 display severe developmental defects as well as induced stress responses. Reduction of NAA50 expression results in arrested stem and root growth as well as senescence. Furthermore, our results demonstrate that the loss of NAA50 results in constitutive ER stress signaling, indicating that NAA50 may be required for the suppression of ER stress. This work establishes NAA50 as essential for plant development and the suppression of stress responses, potentially through the regulation of ER stress.

show abstract

Section: Naa50 Is Required For Growth and The Suppression Of Stress Rsupporting

confidence: 92%

Section: Arabidopsis Naa50 Is Highly Conserved and Essential For Devementioning

confidence: 72%

Section: Naa50 Is Required For Growth and The Suppression Of Stress Rmentioning

confidence: 99%

Section: Naa50 Is Required For Growth and The Suppression Of Stress Rmentioning

confidence: 99%

See 2 more Smart Citations

Loss of the Acetyltransferase NAA50 Induces Endoplasmic Reticulum Stress and Immune Responses and Suppresses Growth

Neubauer

Innes

2020

Plant Physiol.

View full text Add to dashboard Cite

show abstract

“…However, hNAA20 and hNAA25 share only ~40% and ~20% sequence identity with the Candida albicans homologue [57], respectively. Moreover, nearly all biological studies of NatB have been conducted in Saccharomyces cerevisiae [58][59][60], Arabidopsis [61,62], mouse [63] and human [17,[43][44][45] as model organisms. As a result, the mode of human NatB-mediated catalysis and aSyn-specific NatB recognition remains unresolved.…”

Section: Introductionmentioning

confidence: 99%

Molecular Basis for N-terminal Alpha-Synuclein Acetylation by Human NatB

Marmorstein

Deng

Pan

et al. 2020

Preprint

View full text Add to dashboard Cite

NatB is one of three major N-terminal acetyltransferase (NAT) complexes (NatA-NatC), which co-translationally acetylate the N-termini of eukaryotic proteins. Its substrates account for about 21% of the human proteome, including well known proteins such as actin, tropomyosin, CDK2, and αsynuclein (aSyn). Human NatB (hNatB) mediated N-terminal acetylation of αSyn has been demonstrated to play key roles in Parkinson's disease pathogenesis and as a potential therapeutic target for hepatocellular carcinoma.Here we report the cryo-EM structure of hNatB bound to a CoA-aSyn conjugate, together with structure-guided analysis of mutational effects on catalysis. This analysis reveals functionally important differences with human NatA and Candida albicans NatB, resolves key hNatB protein determinants for aSyn Nterminal acetylation, and identifies important residues for substrate-specific recognition and acetylation by NatB enzymes. These studies have implications for developing small molecule NatB probes and for understanding the mode of substrate selection by NAT enzymes.

show abstract

Effects of environmental stress factors on the actin cytoskeleton of fungi and plants: Ionizing radiation and ROS

Stephan

2023

Cytoskeleton

View full text Add to dashboard Cite

Actin is an abundant and multifaceted protein in eukaryotic cells that has been detected in the cytoplasm as well as in the nucleus. In cooperation with numerous interacting accessory‐proteins, monomeric actin (G‐actin) polymerizes into microfilaments (F‐actin) which constitute ubiquitous subcellular higher order structures. Considering the extensive spatial dimensions and multifunctionality of actin superarrays, the present study analyses the issue if and to what extent environmental stress factors, specifically ionizing radiation (IR) and reactive oxygen species (ROS), affect the cellular actin‐entity. In that context, this review particularly surveys IR‐response of fungi and plants. It examines in detail which actin‐related cellular constituents and molecular pathways are influenced by IR and related ROS. This comprehensive survey concludes that the general integrity of the total cellular actin cytoskeleton is a requirement for IR‐tolerance. Actin's functions in genome organization and nuclear events like chromatin remodeling, DNA‐repair, and transcription play a key role. Beyond that, it is highly significant that the macromolecular cytoplasmic and cortical actin‐frameworks are affected by IR as well. In response to IR, actin‐filament bundling proteins (fimbrins) are required to stabilize cables or patches. In addition, the actin‐associated factors mediating cellular polarity are essential for IR‐survivability. Moreover, it is concluded that a cellular homeostasis system comprising ROS, ROS‐scavengers, NADPH‐oxidases, and the actin cytoskeleton plays an essential role here. Consequently, besides the actin‐fraction which controls crucial genome‐integrity, also the portion which facilitates orderly cellular transport and polarized growth has to be maintained in order to survive IR.

show abstract

NatB-Mediated N-Terminal Acetylation Affects Growth and Biotic Stress Responses

Cited by 53 publications

References 60 publications

Loss of the Acetyltransferase NAA50 Induces Endoplasmic Reticulum Stress and Immune Responses and Suppresses Growth

Loss of the Acetyltransferase NAA50 Induces Endoplasmic Reticulum Stress and Immune Responses and Suppresses Growth

Molecular Basis for N-terminal Alpha-Synuclein Acetylation by Human NatB

Effects of environmental stress factors on the actin cytoskeleton of fungi and plants: Ionizing radiation and ROS

Contact Info

Product

Resources

About