National Heart, Lung, and Blood Institute and Building Respiratory Epithelium and Tissue for Health (BREATH) Consortium Workshop Report: Moving Forward in Lung Regeneration

Hynds, Robert E.; Zacharias, William J.; Nikolić, Marko; Königshoff, Mélanie; Eickelberg, Oliver; Gosens, Reinoud; Coppi, Paolo De; Janes, Sam M.; Morrisey, Edward E; Ryan, Amy L.; Stripp, Barry R.; Sun, Xin; Kim, Carla F.; Lin, Qing

doi:10.1165/rcmb.2020-0397ws

Cited by 2 publications

(2 citation statements)

References 83 publications

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“…A comprehensive understanding of human lung development on a cellular and molecular level will facilitate the development of new therapeutic strategies for effective lung regeneration and repair ( 1 , 2 ). In the future, this may offer an alternative to lung transplantation to the many patients suffering with end-stage respiratory failure, the third highest cause of non-communicable disease deaths worldwide ( 3 ).…”

Section: Introductionmentioning

confidence: 99%

Early human lung immune cell development and its role in epithelial cell fate

Barnes,

Yoshida,

et al. 2023

Sci. Immunol.

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Studies of human lung development have focused on epithelial and mesenchymal cell types and function, but much less is known about the developing lung immune cells, even though the airways are a major site of mucosal immunity after birth. An unanswered question is whether tissue-resident immune cells play a role in shaping the tissue as it develops in utero. Here, we profiled human embryonic and fetal lung immune cells using scRNA-seq, smFISH, and immunohistochemistry. At the embryonic stage, we observed an early wave of innate immune cells, including innate lymphoid cells, natural killer cells, myeloid cells, and lineage progenitors. By the canalicular stage, we detected naive T lymphocytes expressing high levels of cytotoxicity genes and the presence of mature B lymphocytes, including B-1 cells. Our analysis suggests that fetal lungs provide a niche for full B cell maturation. Given the presence and diversity of immune cells during development, we also investigated their possible effect on epithelial maturation. We found that IL-1β drives epithelial progenitor exit from self-renewal and differentiation to basal cells in vitro. In vivo, IL-1β–producing myeloid cells were found throughout the lung and adjacent to epithelial tips, suggesting that immune cells may direct human lung epithelial development.

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Section: Introductionmentioning

confidence: 99%

Early human lung immune cell development and its role in epithelial cell fate

Barnes,

Yoshida,

et al. 2023

Sci. Immunol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…A comprehensive understanding of human lung development on a cellular and molecular level is required to facilitate the discovery of novel therapeutic strategies for establishing effective lung regeneration and repair 1,2 . The ability to regenerate human lung tissue in the future may offer an alternative to lung transplantation to the many patients suffering with end-stage respiratory failure, the third highest cause of non-communicable disease deaths worldwide 3 .…”

Section: Introductionmentioning

confidence: 99%

Early human lung immune cell development and its role in epithelial cell fate

Barnes

Yoshida

et al. 2022

Preprint

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During human development, lungs develop their roles of gas exchange and barrier function. Recent single cell studies have focused on epithelial and mesenchymal cell types, but much less is known about the developing lung immune cells, although the airways are a major site of mucosal immunity after birth. An open question is whether tissue-resident immune cells play a role in shaping the tissue as it develops in utero. In order to address this, we profiled lung immune cells using scRNAseq, smFISH and immunohistochemistry. At the embryonic stage, we observed an early wave of innate immune cells, including ILCs, NK, myeloid cells and lineage progenitors. By the canalicular stage, we detected naive T lymphocytes high in cytotoxicity genes, and mature B lymphocytes, including B1 cells. Our analysis suggests that fetal lungs provide a niche for full B cell maturation. Given the abundance of immune cells, we investigated their possible effect on epithelial maturation and found that IL-1β drives epithelial progenitor exit from self-renewal and differentiation to basal cells in vitro. In vivo, IL-1β-producing myeloid cells were found adjacent to epithelial tips, suggesting that immune cells may direct the developing lung epithelium.

show abstract

National Heart, Lung, and Blood Institute and Building Respiratory Epithelium and Tissue for Health (BREATH) Consortium Workshop Report: Moving Forward in Lung Regeneration

Cited by 2 publications

References 83 publications

Early human lung immune cell development and its role in epithelial cell fate

Early human lung immune cell development and its role in epithelial cell fate

Early human lung immune cell development and its role in epithelial cell fate

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