Tea plant is an acidophilic plant, and soil pH has an important effect on the absorption and enrichment of elements, tea plant growth and quality. In this study, rhizosphere soils and leaves of tea plants from 30 tea plantations were collected to determine soil pH and multi-element content of soil and leaves of tea plants, to obtain and validate key elements that are enriched by pH affecting tea plants, and to analyze the effects of pH on the growth and quality of tea plants. The results showed that soil pH significantly affected the enrichment of 15 elements by tea plants, and the enrichment coefficients of 11 elements (C, Mg, Si, N, P, Mn, Sr, Cd, S, Ca and Sb) tended to increase significantly with the increase of soil pH, while the opposite was true for the other four elements (Cu, Rb, Ba and Al). TOPSIS analysis showed that soil pH had the greatest effect on tea plant enrichment of seven elements, namely N (100%), Mn (43.32%), C (39.22%), P (27.66%), Sr (15.30%), Mg (13.41%) and Ba (10.47%). Pot experiments with tea seedlings also verified that soil pH significantly affected the enrichment of tea leaves for seven key elements. Moreover, with the increase of soil pH, the growth indexes, photosynthesis indexes and quality indexes of tea seedlings showed a significant upward trend. Interaction analysis showed that the enhanced enrichment of N, Mn, C, P, Sr and Mg by tea plants was beneficial to increase the photosynthetic capacity of tea plants, promote the growth of tea plants and improve the quality of tea leaves. This study provides an important theoretical basis for the cultivation and management of tea plants.