Nationwide study of Escherichia coli producing extended-spectrum β-lactamases TEM, SHV and CTX-M in Turkey

Çiçek, Ayşegül Çopur; Saral, A.; Düzgün, Azer Özad; Yaşar, Ekrem; Çizmeci, Zeynep; Balci, Pervin Ozlem; Sarı, Fatma Nur; Fırat, Mehmet Ziya; Altintop, Yasemin Ay; Ak, Sibel; Çalışkan, Ahmet; Yıldız, Nazan; Sancaktar, Metin; Budak, Emine Esra; Ertürk, Ayşe; Özgümüş, Osman Birol; Sandallı, Cemal

doi:10.1038/ja.2013.72

Cited by 25 publications

(8 citation statements)

References 19 publications

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“…CTX-M-15 dominates in Germany and the Netherlands, but more recent studies show an increased proportion of CTX-M-1 compared to CTX-M-14 (Voets et al, 2012; Pietsch et al, 2017). CTX-M-14 has been found to be less prevalent in most countries with some exceptions [China, South-East Asia, South Korea, Japan, and Spain; (Onnberg et al, 2011; Copur Cicek et al, 2013; Helldal et al, 2013; Bevan et al, 2017)]. Although we found CTX-M-14 in all investigated countries it was less prevalent than CTX-M-15.…”

Section: Discussioncontrasting

confidence: 66%

Phenotypic and Molecular Epidemiology of ESBL-, AmpC-, and Carbapenemase-Producing Escherichia coli in Northern and Eastern Europe

et al. 2019

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Extended-spectrum beta-lactamases (ESBL) and AmpC producing-Escherichia coli have spread worldwide, but data about ESBL-producing-E. coli in the Northern and Eastern regions of Europe is scant. The aim of this study has been to describe the phenotypical and molecular epidemiology of different ESBL/AmpC/Carbapenemases genes in E. coli strains isolated from the Baltic States (Estonia, Latvia, and Lithuania), Norway and St. Petersburg (Russia), and to determine the predominant multilocus sequence type and single nucleotide polymorphisms diversity of E. coli isolates deduced by whole genome sequencing (WGS). A total of 10,780 clinical E. coli strains were screened for reduced sensitivity to third-generation cephalosporins. They were collected from 21 hospitals located in Estonia, Latvia, Lithuania, Norway and St. Petersburg during a 5 month period in 2012. The overall prevalence of ESBL/AmpC strains was 4.7% by phenotypical test and 3.9% by sequencing. We found more strains with the ESBL/AmpC phenotype and genotype in St. Petersburg and Latvia than other countries. Of phenotypic E. coli strains, 85% contained confirmed ESBL genes (including blaCTX–M, blaTEM–29, blaTEM–71), AmpC genes (blaCMY–59, blaACT–12/–15/–20, blaESC–6, blaFEC–1, blaDHA–1), or carbapenemase genes (blaNDM–1). blaCTX–M–1, blaCTX–M–14 and blaCTX–M–15 were found in all countries, but blaCTX–M–15 prevalence was higher in Latvia than in St. Petersburg (Russia), Estonia, Norway and Lithuania. The dominating AmpC genes were blaCMY–59 in the Baltic States and Norway, and blaDHA–1 in St. Petersburg. E. coli strains belonged to 83 different sequence types, of which the most prevalent was ST131 (40%). In conclusion, we generally found low ESBL/AmpC/Carbapenemase prevalence in E. coli strains isolated in Northern/Eastern Europe. However, several inter-country differences in distribution of particular genes and multilocus sequence types were found.

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Section: Discussioncontrasting

confidence: 66%

Phenotypic and Molecular Epidemiology of ESBL-, AmpC-, and Carbapenemase-Producing Escherichia coli in Northern and Eastern Europe

et al. 2019

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“…Previously, the rates of bla SHV containing Enterobacterales with a focus on uropathogenic isolates were reported 12.2% to 54.8% by Iranian authors. [24][25][26][27][28][29] Meanwhile, several authors have shown the global spared of SHV type β-lactamases in uro- pathogenic Enterobacterales from China, 30 India, 31 Saudi Arabia, 32 Turkey, 33 Ukraine, 34 and Morocco. 35 In our results, 4.1% of uropathogenic Enterobacterales contained bla NDM-1, which to the best of our knowledge, is the first prevalence of this gene in Guilan province (North of Iran).…”

Section: Discussionmentioning

confidence: 99%

The Phylogenetic Relatedness of blaNDM-1 Harboring Extended-Spectrum β-Lactamase Producing Uropathogenic Escherichia coli and Klebsiella pneumoniae in the North of Iran

Jamali¹,

Tavakoly²,

Mojtahedi³

et al. 2020

IDR

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Background: Escherichia coli and Klebsiella pneumoniae as an important part of Enterobacterales family are important causes of both community-and hospital-acquired infections. The present study aimed to investigate the prevalence of antibiotics resistance and molecular characteristics of uropathogenic isolates of E. coli and K. pneumoniae in Iranian patients. Methods: This cross-sectional study performed on 223 Escherichia coli and 68 Klebsiella pneumoniae isolates obtained from hospitalized patients in the north of Iran. The isolates were identified by standard microbiologic tests and confirmed by API 20E strip. Disk diffusion method was applied to determine antibiotic susceptibility pattern. The presence of β-lactamases encoding genes was evaluated by PCR method. Analysis of the mutations and homology among sequences was done by the CLC sequence viewer (Qiagen, Denmark), and phylogenetic trees were constructed by the neighbor-joining method (Bootstrap: 1000 times). Results: The overall rates of extended-spectrum β-lactamases (ESBLs)-producing E. coli and K. pneumoniae isolates were 37.7% and 32.4%, respectively. The overall presence of bla SHV , bla NDM-1 , and bla OXA-1 genes was detected in 16 (5.5%), 12 (4.1%), and 48 (16.4%) of isolates, respectively. The neighbor-joining analysis for E. coli KU985246.1 strain showed that the most related bla NDM-1 sequences were from China, Singapore, UK, Thailand, and Bangladesh. While K. pneumoniae KU985245.1 strains were mostly related to bla NDM-1 sequences form Myanmar, and China. Conclusion: In summary, the remarkable rate of ESBL-producing uropathogenic Enterobacterales along with the first prevalence of NDM-1 β-lactamases can be a serious concern in our region.

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“…CTX-M-15 were reported in healthy dogs by several studies [17, 24, 34, 41, 54]. CTX-M-15 is also the most commonly reported enzyme type in E. coli isolates from human clinical samples [2, 13, 19]. Recent studies in Turkey shows presence of this enzyme among ESBL producing E. coli isolates in cattle and retail meat [3, 37].…”

Section: Discussionmentioning

confidence: 99%

Prevalence and molecular characterization of extended-spectrum β-lactamase (ESBL) and plasmidic AmpC β-lactamase (pAmpC) producing Escherichia coli in dogs

Aslantaş

Yılmaz

2017

The Journal of Veterinary Medical Science

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This study aimed to determine the prevalence of fecal carriage of extended spectrum β-lactamase (ESBL) and/or plasmidic AmpC β-lactamase (pAmpC) producing Escherichia coli among dogs (n=428) in Turkey. Polymerase chain reaction (PCR) and sequencing were used to characterize genes encoding β-lactamase and plasmid mediated quinolone resistance (PMQR). Antimicrobial susceptibility testing and PCRs for virulence genes and phylogenetic groups were also performed. Cefotaxime resistant E. coli isolates were detected in 95 (22.2%) of the swab samples. Sequencing analysis results showed occurrence of various β-lactamase genes: blaCTX-M-15 (62), blaTEM-1b (42), blaCMY-2 (22), blaCTX-M-3 (16), blaCTX-M-1 (15), blaOXA-1 (9) and blaSHV-12 (3) alone or in combination. The most frequently encountered phylogenetic group was group A1 (35.8%), followed by group D2 (22.1%), B1 (15.8%), D1 (9.5%), A0 (7.4%), B22 (5.3%) and B23 (4.2%), respectively. PMQR genes, aac(6’)-Ib-cr, qnrS1 and qnrB10 were detected in 25.3, 10.5 and 1.1% of the isolates, respectively. While all isolates were susceptible to imipenem and amikacin, resistance rates to non-β-lactam antibiotics ranged from 20.0% for tobramycin to 56.8% for tetracycline. The virulence genes were only detected in 34 (36.2%) of the isolates and this isolates carried single or various combination of virulence genes of iucD, papC, papE, f17a-A and eaeA. Four isolates were identified as human virulent pandemic CTX-M-15 producing E. coli clone O25b:ST131/B2. To the best of our knowledge, this is the first study to show fecal carriage of ESBL/pAmpC type β-lactamase producing E. coli isolates among dogs in Turkey.

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Nationwide study of Escherichia coli producing extended-spectrum β-lactamases TEM, SHV and CTX-M in Turkey

Cited by 25 publications

References 19 publications

Phenotypic and Molecular Epidemiology of ESBL-, AmpC-, and Carbapenemase-Producing Escherichia coli in Northern and Eastern Europe

Phenotypic and Molecular Epidemiology of ESBL-, AmpC-, and Carbapenemase-Producing Escherichia coli in Northern and Eastern Europe

<p>The Phylogenetic Relatedness of <em>bla</em>N<sub>DM-1</sub> Harboring Extended-Spectrum β-Lactamase Producing Uropathogenic <em>Escherichia coli</em> and <em>Klebsiella pneumoniae</em> in the North of Iran</p>

Prevalence and molecular characterization of extended-spectrum β-lactamase (ESBL) and plasmidic AmpC β-lactamase (pAmpC) producing <i>Escherichia coli</i> in dogs

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