Native ambient mass spectrometry of intact protein assemblies directly from<i>Escherichia coli</i>colonies

Du, Yuying; May, Robin C.; Cooper, Helen J.

doi:10.1039/d2cc02085h

Cited by 4 publications

(2 citation statements)

References 33 publications

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“…Characterization of phospholipids in bacterial cultures has also been achieved with DESI‐MS, achieving an impressive sample analysis time of less than 10 s. 154 The analysis of bacterial species has primarily targeted smaller metabolites produced by microbes, though a recent study used LESA‐MS for the detection of proteins in E. coli colonies. 155 Through the combination of electroporation to release proteins from cells followed by LESA‐MS, intact protein assemblies up to 50 kDa could be detected directly from growing colonies. The analysis of biological materials is perhaps the largest area of research utilizing AIMS technology, and this section is by no means exhaustive.…”

Section: Applications Of Aimsmentioning

confidence: 99%

Applications of ambient ionization mass spectrometry in 2022: An annual review

Rankin‐Turner

Sears

Heaney

2023

Analytical Science Advances

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The development of ambient ionization mass spectrometry (AIMS) has transformed analytical science, providing the means of performing rapid analysis of samples in their native state, both in and out of the laboratory. The capacity to eliminate sample preparation and pre‐MS separation techniques, leading to true real‐time analysis, has led to AIMS naturally gaining a broad interest across the scientific community. Since the introduction of the first AIMS techniques in the mid‐2000s, the field has exploded with dozens of novel ion sources, an array of intriguing applications, and an evident growing interest across diverse areas of study. As the field continues to surge forward each year, ambient ionization techniques are increasingly becoming commonplace in laboratories around the world. This annual review provides an overview of AIMS techniques and applications throughout 2022, with a specific focus on some of the major fields of research, including forensic science, disease diagnostics, pharmaceuticals and food sciences. New techniques and methods are introduced, demonstrating the unwavering drive of the analytical community to further advance this exciting field and push the boundaries of what analytical chemistry can achieve.

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Section: Applications Of Aimsmentioning

confidence: 99%

Applications of ambient ionization mass spectrometry in 2022: An annual review

Rankin‐Turner

Sears

Heaney

2023

Analytical Science Advances

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“…Despite the huge strides made for the analysis of native proteins and non-covalent complexes from tissue samples, until recently very little has been reported from bacterial colonies. However, electroporation followed by LESA sampling with ammonium buffered solvent systems recently enabled detection of superoxide dismutase homodimers up to 50 kDa from E. coli [ 55 ].…”

Section: Electrospray Ionisation (Esi)-based Approachesmentioning

confidence: 99%

Surface-sampling mass spectrometry to study proteins and protein complexes

Wong

Greatorex

Gidman

et al. 2023

Essays in Biochemistry

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This review aims to summarise the current capabilities of surface mass spectrometry (MS) approaches that offer intact protein analysis, and that of non-covalent complexes. Protein analysis is largely achieved via matrix-assisted laser desorption/ionisation (MALDI), which is in itself a surface analysis approach or solvent-based electrospray ionisation (ESI). Several surface sampling approaches have been developed based on ESI, and those that have been used for intact protein analysis will be discussed below. The extent of protein coverage, top-down elucidation, and probing of protein structure for native proteins and non-covalent complexes will be discussed for each approach. Strategies for improving protein analysis, ranging from sample preparation, and sampling methods to instrument modifications and the inclusion of ion mobility separation in the workflow will also be discussed. The relative benefits and drawbacks of each approach will be summarised, providing an overview of current capabilities.

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