Native Autoantigen Complex Detects Pemphigoid Autoantibodies

Mai, Shoko; Izumi, Kiyotaka; Mai, Yosuke; Natsuga, Ken; Ishii, Norito; Sawamura, Daisuke; Schauer, Franziska; Kiritsi, Dimitra; Nishie, Wataru; Ujiie, Hideyuki

doi:10.1016/j.xjidi.2023.100193

Cited by 7 publications

(4 citation statements)

References 54 publications

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“…HaCaT cells were originally derived from the back skin of a 62-year-old male and not from a single cell 14 ; therefore, heterogeneous cells might have formed the regions of high or low cell densities. To rule out this possibility, we performed single-cell cloning of HaCaT cells by integrating a puromycin cassette and the Cas9 gene into the cells, the latter of which enabled further knockout experiments 15 . Single-cell-cloned HaCaT cells developed a self-organized pattern similar to that observed in the parental cells (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

“…HaCaT cells 14 were originally obtained from Dr. Norbert Fussenig (German Cancer Research Center, Heidelberg, Germany), and the cell identity was confirmed with the Cell Culture STR profile (Biologica, Nagoya, Japan). Single-cell-cloned HaCaT cells with stable Cas9 expression were established previously 15 . Briefly, HaCaT cells were transfected with pLenti-EF1a-Cas9-Puro lentiviral particles (Applied Biological Materials, Richmond, Canada) and selected with 1 µg/mL puromycin (Thermo Fischer Scientific, Waltham, Massachusetts, US).…”

Section: Cellsmentioning

confidence: 99%

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Cell–cell adhesion drives patterning in stratified epithelia

Mai,

Kobayashi,

Kitahata

et al. 2023

Preprint

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Epithelia consist of proliferating and differentiating cells that often display patterned arrangements. However, the mechanism regulating these spatial arrangements remains unclear. Here, we show that cell–cell adhesion dictates multicellular patterning in stratified epithelia. When cultured keratinocytes, a type of epithelial cell in the skin, are subjected to starvation, they spontaneously develop a pattern characterized by areas of high and low cell density. Pharmacological and knockout experiments show that adherens junctions are essential for patterning, whereas mathematical modeling indicates that cell–cell adhesion alone is sufficient to form regions with high/low cell density. This phenomenon, called cell–cell adhesion-induced patterning (CAIP), influences cell differentiation and proliferation through Yes-associated protein modulation. Starvation, which induces CAIP, enhances the stratification of the epithelia. These findings highlight the intrinsic self-organizing property of epithelial cells and indicate that CAIP modulation might promote wound healing in clinical settings.

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Section: Resultsmentioning

confidence: 99%

Section: Cellsmentioning

confidence: 99%

Cell–cell adhesion drives patterning in stratified epithelia

Mai,

Kobayashi,

Kitahata

et al. 2023

Preprint

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“…We recently reported a novel enzyme-linked immunosorbent assay (ELISA) system that utilizes the epidermal basement membrane zone protein complex derived from HaCaT immortalized keratinocytes with a DDDDK-tag knocked into COL17 by gene-editing. 6 We purified and acquired the native COL17 complex by immunoprecipitation. 6 Since the native COL17 complex contains ITG α6β4 as binding partners of COL17 (Figure 1a), the native COL17 complex ELISA can detect autoantibodies targeting ITG α6β4.…”

mentioning

confidence: 99%

“…6 We purified and acquired the native COL17 complex by immunoprecipitation. 6 Since the native COL17 complex contains ITG α6β4 as binding partners of COL17 (Figure 1a), the native COL17 complex ELISA can detect autoantibodies targeting ITG α6β4. 6 Here, we demonstrate the diagnostic potential of the native COL17 complex ELISA and its ability to monitor disease activity by quantifying circulating autoantibodies against ITG β4.…”

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confidence: 99%

Native collagen XVII complex ELISA: An approach for diagnosis and monitoring of anti‐integrin β4 mucous membrane pemphigoid

Mai,

Izumi,

Itamoto

et al. 2023

Acad Dermatol Venereol

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Dear Editor, Mucous membrane pemphigoid (MMP) is a chronic and heterogeneous autoimmune blistering disease that predominantly affects mucous membranes, although it can also involve the skin. 1 Target molecules of MMP autoantibodies include various basement membrane zone proteins such as collagen XVII (COL17, BP180), laminin-332, BP230, integrin (ITG) α6β4 and collagen VII (COL7) (Figure 1a). 2 Autoantibodies of MMP patients are typically low titre 1 ; thus, it is difficult to utilize antibody titre as a biomarker of disease activity in MMP. Some studies have suggested a potential association between autoantibodies against ITG β4 and pure ocular MMP. 3,4 However, the routine analysis of anti-ITG β4 autoantibody is not widely available due to the limited availability of specialized laboratories with in-house assay methods. 5 Furthermore, the prevalence and specific phenotype of anti-ITG β4 antibodies in MMP patients have not been clearly determined so far. 5

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