Native ectomycorrhizal fungi from the endangered pine rocklands are superior symbionts to commercial inoculum for slash pine seedlings

Karlsen‐Ayala, Elena; Smith, Matthew E.; Askey, Bryce C.; Gazis, Romina

doi:10.1007/s00572-022-01092-3

Cited by 10 publications

(3 citation statements)

References 73 publications

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“…Similarly, quantifying the percent of individual root tips colonized by Rhizopogon for field or in vitro assays can be challenging due to their typically coralloid morphology (Kennedy & Peay, 2007). For fruit bodies, morphological differentiation of Rhizopogon is notoriously complicated by a lack of variable diagnostic characters, which confound field identification, and contribute to the instability of species concepts developed in the premolecular era (Bidartondo & Bruns, 2002; Bubriski & Kennedy, 2014; Karlsen‐Ayala et al ., 2022; Koukol et al ., 2022). Although Rhizopogon was long thought to be sister to Suillus , multiple molecular studies have identified fungi in the family Gomphidiaceae as more closely related to Suillus (Grubisha et al ., 2002; Wu et al ., 2020).…”

Section: Taxonomy and Evolutionmentioning

confidence: 99%

Suillus: an emerging model for the study of ectomycorrhizal ecology and evolution

Lofgren,

Nguyen,

Kennedy

et al. 2024

New Phytologist

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SummaryResearch on mycorrhizal symbiosis has been slowed by a lack of established study systems. To address this challenge, we have been developing Suillus, a widespread ecologically and economically relevant fungal genus primarily associated with the plant family Pinaceae, into a model system for studying ectomycorrhizal (ECM) associations. Over the last decade, we have compiled extensive genomic resources, culture libraries, a phenotype database, and protocols for manipulating Suillus fungi with and without their tree partners. Our efforts have already resulted in a large number of publicly available genomes, transcriptomes, and respective annotations, as well as advances in our understanding of mycorrhizal partner specificity and host communication, fungal and plant nutrition, environmental adaptation, soil nutrient cycling, interspecific competition, and biological invasions. Here, we highlight the most significant recent findings enabled by Suillus, present a suite of protocols for working with the genus, and discuss how Suillus is emerging as an important model to elucidate the ecology and evolution of ECM interactions.

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Section: Taxonomy and Evolutionmentioning

confidence: 99%

Suillus: an emerging model for the study of ectomycorrhizal ecology and evolution

Lofgren,

Nguyen,

Kennedy

et al. 2024

New Phytologist

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“…Symbiosis between plants and microorganisms occurs wide in natural ecosystems, affects plant terrestrializations (Puginier et al, 2022), evolution (Batstone, 2022;van Galen et al, 2023), and tolerance to abiotic and biotic stress (Zeng et al, 2022), and widens the habitability ranges of plants (Muñoz and Carneiro, 2022). Among all the beneficial microorganisms symbiosing with plants, five families of microorganisms have been greatly paid on attentions, i.e., arbuscular mycorrhizal fungi (Kaur et al, 2022;Razak and Gange, 2023;Chen W. et al, 2023), ectomycorrhizal fungi (Karlsen-Ayala et al, 2022;Jörgensen et al, 2023;Xiao et al, 2023), root endophytic fungi (Manzur et al, 2022;Sun et al, 2022;Qin et al, 2023), dark septate fungi (Gaber et al, 2023;Wang et al, 2023;Chen S. et al, 2023), and plant growth-promoting rhizobacteria (PGPR) (Ahmad et al, 2022;Gowtham et al, 2022;Zhao et al, 2023). These beneficial microorganisms show strong effects on their plant hosts under drought stress (Ahmad et al, 2022;Gowtham et al, 2022;Zhao et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Colonization of root endophytic fungus Serendipita indica improves drought tolerance of Pinus taeda seedlings by regulating metabolome and proteome

Wu,

Yang,

Wang

et al. 2024

Front. Microbiol.

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Pinus taeda is an important forest tree species for plantations because of its rapid growth and high yield of oleoresins. Although P. taeda plantations distribute in warm and wet southern China, drought, sometime serious and long time, often occurs in the region. To explore drought tolerance of P. taeda and usage of beneficial microorganisms, P. taeda seedlings were planted in pots and were inoculated with root endophytic fungus Serendipita indica and finally were treated with drought stress for 53 d. Metabolome and proteome of their needles were analyzed. The results showed that S. indica inoculation of P. taeda seedlings under drought stress caused great changes in levels of some metabolites in their needles, especially some flavonoids and organic acids. Among them, the levels of eriocitrin, trans-aconitic acid, vitamin C, uric acid, alpha-ketoglutaric acid, vitamin A, stachydrine, coumalic acid, itaconic acid, calceolarioside B, 2-oxoglutaric acid, and citric acid were upregulated more than three times in inoculated seedlings under drought stress, compared to those of non-inoculated seedlings under drought stress. KEGG analysis showed that some pathways were enriched in inoculated seedlings under drought stress, such as flavonoid biosynthesis, ascorbate and aldarate metabolism, C5-branched dibasic acid metabolism. Proteome analysis revealed some specific differential proteins. Two proteins, namely, H9X056 and H9VDW5, only appeared in the needles of inoculated seedlings under drought stress. The protein H9VNE7 was upregulated more than 11.0 times as that of non-inoculated seedlings under drought stress. In addition, S. indica inoculation increased enrichment of water deficient-inducible proteins (such as LP3-1, LP3-2, LP3-3, and dehydrins) and those involved in ribosomal structures (such as A0A385JF23). Meanwhile, under drought stress, the inoculation caused great changes in biosynthesis and metabolism pathways, mainly including phenylpropanoid biosynthesis, cutin, suberine and wax biosynthesis, and 2-oxocarboxylic acid metabolism. In addition, there were positive relationships between accumulation of some metabolites and enrichment of proteins in P. taeda under drought stress. Altogether, our results showed great changes in metabolome and proteome in inoculated seedlings under drought stress and provided a guideline to further study functions of metabolites and proteins, especially those related to drought stress.

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“…Sampled trees were distributed in Pine Rocklands habitats in south Florida, a critically endangered tropical dry forest ecosystem ( Snyder et al 1990 ). This ecosystem is extremely important for animals, birds, reptiles, plants, and microorganisms including mycorrhizae and endophytes ( Platt et al 2010 , Lloyd & Slater 2012 , Possley et al 2016 , Karlsen-Ayala et al 2022 ). Based on morphological and molecular data, we introduce two novel Tolypocladium species.…”

Section: Introductionmentioning

confidence: 99%

Two novel endophytic Tolypocladium species identified from native pines in south Florida

Soares

Karlsen-Ayala

Salvador-Montoya

et al. 2023

Fungal Systematics and Evolution

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This study investigated the incidence and diversity of Tolypocladium within trunks of south Florida slash pines (Pinus densa). Thirty-five isolates were recovered from trunk tissue including living phloem, cambium, and sapwood. Two novel species of Tolypocladium (T. subtropicale and T. trecense) are described here based on morphological and molecular analysis of concatenated LSU, ITS, tef-1, tub, and RPB1 sequences. Our findings expand our understanding of the distribution, diversity, and ecology of this genus and confirm that it is widely spread as an endophyte across ecosystems and hosts. Strains collected in this survey will be used in future bioassays to determine their potential ecological roles as mycoparasites or entomopathogens.

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Native ectomycorrhizal fungi from the endangered pine rocklands are superior symbionts to commercial inoculum for slash pine seedlings

Cited by 10 publications

References 73 publications

Suillus: an emerging model for the study of ectomycorrhizal ecology and evolution

Suillus: an emerging model for the study of ectomycorrhizal ecology and evolution

Colonization of root endophytic fungus Serendipita indica improves drought tolerance of Pinus taeda seedlings by regulating metabolome and proteome

Two novel endophytic Tolypocladium species identified from native pines in south Florida

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