Native extracellular matrix-derived semipermeable, optically transparent, and inexpensive membrane inserts for microfluidic cell culture

Mondrinos, Mark J.; Yi, Yoon-Suk; Wu, Nan-Kun; Ding, Xueting; Huh, Dongeun

doi:10.1039/c7lc00317j

Cited by 79 publications

(95 citation statements)

References 36 publications

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“…Most hydrogel solutions are viscous liquids that can be coated on the surface of another material and peeled off to form biocompatible membranes after gelation. Such membranes are promising modules to serve as tissue–tissue interfaces in OOC platform or form cell sheets by seeding cells on them. To develop complex 3D constructs, a more flexible method is needed to present hierarchical modules in one system.…”

Section: Snapshot Of Hydrogelsmentioning

confidence: 99%

“…C) Thin hydrogel‐based membranes made from collagen and Matrigel to support cells coculture and form tissue–tissue interface. Reproduced with permission . Copyright 2017, Royal Society of Chemistry.…”

Section: Hydrogels In Organs‐on‐a‐chip Engineeringmentioning

confidence: 99%

“…Recently, natural hydrogels (e.g., collagen and Matrigel)‐based membranes have been developed to be incorporated into chip device for engineering human barrier tissues. For example, native ECM‐derived membranes that presented a mass of bonding sites for cell adhesion were created with tunable biophysical properties . They could be integrated with microfluidic chips to provide scaffold for cell culture, and replicate biological interfaces, such as lung air–liquid interface and lung epithelial–stromal interface, contributing to the development of physiologically relevant in vitro tissue models (Figure C).…”

Section: Hydrogels In Organs‐on‐a‐chip Engineeringmentioning

confidence: 99%

“…Several defined hydrogels have been proven to facilitate organoids formation as substitutes to undefined animal‐derived matrix for improving the reproducibility and maturity of organoids by precise control of their componential, architectural, and/or mechanical conditions . With respect to OOC platforms, hydrogels have been applied to improve the physiological relevance of tissue models on chips by replicating 3D matrix, tissue interfaces, vascularized network, cell–cell and cell–ECM interactions in a biomimetic microenvironment …”

Section: Introductionmentioning

confidence: 99%

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Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

et al. 2019

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The major motivation is to better mimic human physiology and functions at multiscales from the molecular to the cellular, tissue, organ or even whole organism level. Current model systems primarily rely on the monolayer cell cultures and animal models. Simplistic monolayer cultures have their advantages, but they are often significantly different in gene expression, epigenetics, and cell function compared to native 3D tissues. [1,2] Also, they often lack cell-cell and cell-matrix interactions, [2,3] leading to the absence of tissue specific properties. Although animal models are widely used in biomedical research, they fail to faithfully predict human responses in a physiologically relevant manner due to the significant species divergences. [4] The limitations of these systems have provided an impetus for the development of alternative cell-based 3D models in vitro that better resemble the complex functionalities of living organs. Over the last several years, organoids and organ-on-a-chip (OOC), representing the major technological breakthrough, have emerged as two distinct model systems to achieve the same goal of building 3D organotypic models in vitro by bridging the gap between animal models and monolayer cultures. These engineered models are different in terms of cell source, tissue composition, architectural variability, functional features, scales, cellular fidelity, etc. Organoids, primarily evolving from developmental principles, refer to 3D multicellular tissues by self-organization of stem cells or organ-specific progenitors, which can recapitulate the intricate architectures and functionalities of in vivo organs. [5][6][7] Recent breakthroughs in organoid technology have enabled the successful generation of a variety of human organoids, such as the brain, [8,9] intestine, [10,11] liver, [12] kidney, [13,14] lung, [15] etc. These near physiological 3D organoids have garnered momentum for their potential applications in human organ development and disease modeling, drug screening and regenerative medicine. The explosion of organoids research has been catalyzed by the significant progress of stem cell biology and availability of human stem cells. In contrast, the OOC, relying on the bioengineering design principle, is a miniaturized in vitro model that can recreate the functional units of living organs on a microfluidic cell culture device using predifferentiated cells, often cell lines. [1,16] The OOC is primarily evolved from the convergence of tissue engineering and microfabricated technologies, which is characterized by the capability to simulate cellular microenvironment by precise control over fluid flow, mechanical forces and biochemical factors. [4,17] Remarkable progress has been made Significant advances in materials, microscale technology, and stem cell biology have enabled the construction of 3D tissues and organs, which will ultimately lead to more effective diagnostics and therapy. Organoids and organs-on-a-chip (OOC), evolved from developmental biology and bioengineering principles, have e...

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Section: Snapshot Of Hydrogelsmentioning

confidence: 99%

Section: Hydrogels In Organs‐on‐a‐chip Engineeringmentioning

confidence: 99%

Section: Hydrogels In Organs‐on‐a‐chip Engineeringmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

et al. 2019

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“…More recently, collagen membranes have been integrated into microfluidic devices for use as cell culture substrates. These membranes were either cast 21,22 , compressed 23 or spin-coated 24 on a PDMS surface prior to being sandwiched between two microfluidic structures. The resulting thicknesses of these membranes, not designed to be stretched, were between 15 and 30m.…”

mentioning

confidence: 99%

Second-generation lung-on-a-chip array with a stretchable biological membrane

Zamprogno

Wüthrich

Achenbach

et al. 2019

Preprint

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The complex architecture of the lung parenchyma and the air-blood barrier is difficult to mimic in-vitro. Recently reported lung-on-a-chips used a thin, porous and stretchable PDMS membrane, to mimic the air-blood barrier and the rhythmic breathing motions. However, the nature, the properties and the size of this PDMS membrane differ from the extracellular matrix of the distal airways. Here, we present a second-generation lung-on-a-chip with an array of in vivolike sized alveoli and a stretchable biological membrane. This nearly absorption free membrane allows mimicking in vivo functionality of the lung parenchyma at an unprecedented level. The air-blood barrier is constituted by human primary lung alveolar epithelial cells from several patients and co-cultured with primary lung endothelial cells. Typical markers of lung alveolar epithelial cells could be observed in the model, while barrier properties were preserved for up to three weeks. This advanced lung alveolar model reproduces some key features of the lung

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Advanced In Vitro Lung Models for Drug and Toxicity Screening: The Promising Role of Induced Pluripotent Stem Cells

Moreira¹,

Müller

Costa³

et al. 2021

Advanced Biology

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Respiratory diseases are among the global leading causes of morbidity and mortality. Acute conditions arising from infection, such as pneumonia and tuberculosis, affect millions of people worldwide, the latter being the most common lethal infectious disease with 1.4 million annual deaths. [1] Upon infection, the severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), which caused a worldwide pandemic, leads to the clinical picture of the coronavirus disease-2019 (COVID-19) with possibly severe and life-threatening progression. Lung cancer is the most frequently diagnosed malignancy and the main cause of cancer-related death, [2] with markedly low survival rates especially when the diagnosis is performed at an advanced state of the disease. [3] Moreover, chronic respiratory diseases (CRDs), including chronic obstructive pulmonary disease (COPD), asthma, and interstitial lung disease (ILD), have consistently received less attention in comparison to other non-communicable diseases, continuing to exert a considerable socioeconomic impact. [4,5] Risk factors for the development of respiratory diseases include, for example, tobacco use and second-hand smoke, as well as exposure to air-pollutants, which has been accentuated with the widespread use of fossil fuels. [4] It is clear that a great number of these risks can be mitigated by lifestyle changes, promoted by anti-tobacco campaigns already in place at a global scale and by the use of renewable, clean energy sources, and two recent studies indicate that the age-standardized incidence and prevalence of CRDs has decreased from 1990 to 2017. [4,5] However, the risk of developing CRDs, particularly COPD, appears to increase steeply with age; [4,5] most strikingly, these diseases were the third leading cause of death in 2017 [4] and potentially in 2020. [6] In addition to microbial, environmental, and lifestyle-related factors, a large number of lung diseases have a genetic origin. [7] Cystic fibrosis (CF), for example, is an incurable hereditary disorder known to originate from different mutations in the gene coding for the CF transmembrane conductance regulator (CFTR), an ion transporter, resulting in severe alterations in pulmonary physiology that culminate in impaired lung function, respiratory distress and, ultimately, death. [8,9]

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Native extracellular matrix-derived semipermeable, optically transparent, and inexpensive membrane inserts for microfluidic cell culture

Cited by 79 publications

References 36 publications

Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

Second-generation lung-on-a-chip array with a stretchable biological membrane

Advanced In Vitro Lung Models for Drug and Toxicity Screening: The Promising Role of Induced Pluripotent Stem Cells

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