Native feedstock options for the polyhydroxyalkanoate industry in Europe: A review

Gutschmann, Björn; Huang, Boyang; Santolin, Lara; Thiele, Isabel; Neubauer, Peter; Riedel, Sebastian L.

doi:10.1016/j.micres.2022.127177

Cited by 24 publications

(8 citation statements)

References 95 publications

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“…Beyond their direct engagement in PHA biosynthesis, VFAs can be produced in large amounts from acidogenic fermentation of numerous organic waste streams, which can ensure a sufficient supply to replace expensive conventional carbon sources such as corn, wheat, sugar beet, etc. [ 12 , 13 ]. The use of VFAs in the cultivation of PHA-bearing bacteria has been conducted in several studies, showing promising results in PHA yields [ 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Application of Immersed Membrane Bioreactor for Semi-Continuous Production of Polyhydroxyalkanoates from Organic Waste-Based Volatile Fatty Acids

Mahboubi

Root³

et al. 2023

Membranes

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Volatile fatty acids (VFAs) appear to be an economical carbon feedstock for the cost-effective production of polyhydroxyalkanoates (PHAs). The use of VFAs, however, could impose a drawback of substrate inhibition at high concentrations, resulting in low microbial PHA productivity in batch cultivations. In this regard, retaining high cell density using immersed membrane bioreactor (iMBR) in a (semi-) continuous process could enhance production yields. In this study, an iMBR with a flat-sheet membrane was applied for semi-continuous cultivation and recovery of Cupriavidus necator in a bench-scale bioreactor using VFAs as the sole carbon source. The cultivation was prolonged up to 128 h under an interval feed of 5 g/L VFAs at a dilution rate of 0.15 (d−1), yielding a maximum biomass and PHA production of 6.6 and 2.8 g/L, respectively. Potato liquor and apple pomace-based VFAs with a total concentration of 8.8 g/L were also successfully used in the iMBR, rendering the highest PHA content of 1.3 g/L after 128 h of cultivation. The PHAs obtained from both synthetic and real VFA effluents were affirmed to be poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with a crystallinity degree of 23.8 and 9.6%, respectively. The application of iMBR could open an opportunity for semi-continuous production of PHA, increasing the feasibility of upscaling PHA production using waste-based VFAs.

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Section: Introductionmentioning

confidence: 99%

Application of Immersed Membrane Bioreactor for Semi-Continuous Production of Polyhydroxyalkanoates from Organic Waste-Based Volatile Fatty Acids

Mahboubi

Root³

et al. 2023

Membranes

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“…The key problem for increasing production volumes and expanding the scope of PHAs is to reduce their cost by using available carbon raw materials. Potentially, various substrates can serve as raw materials for PHA synthesis, as individual compounds [19][20][21][22][23][24][25][26][27] and, of particular significance, various byproducts (products of the processing and hydrolysis of plant raw materials, byproducts from different industries [27][28][29][30][31]. The potential for PHA synthesis in byproducts of various origins has significant potential for "The Circular Economy" [14,[32][33][34].…”

Section: Introductionmentioning

confidence: 99%

Properties of Degradable Polyhydroxyalkanoates Synthesized from New Waste Fish Oils (WFOs)

Zhila,

Kiselev,

Volkov

et al. 2023

IJMS

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The synthesis of PHA was first investigated using WFOs obtained from smoked-sprat heads, substandard fresh sprats, and fresh mackerel heads and backbones. All the WFOs ensured the growth of the wild-type strain Cupriavidus necator B-10646 and the synthesis of PHA, regardless of the degree of lipid saturation (from 0.52 to 0.65) and the set and ratio of fatty acids (FA), which was represented by acids with chain lengths from C14 to C24. The bacterial biomass concentration and PHA synthesis were comparable (4.1–4.6 g/L and about 70%) when using WFO obtained from smoked-sprat heads and fresh mackerel, and it was twice as high as the bacterial biomass concentration from the fresh sprat waste. This depended on the type of WFO, the bacteria synthesized P(3HB) homopolymer or P(3HB-co-3HV-co-3HHx) copolymer, which had a lower degree of crystallinity (Cx 71%) and a lower molecular weight (Mn 134 kDa) compared to the P(3HB) (Mn 175–209 kDa and Cx 74–78%) at comparable temperatures (Tmelt and Tdegr of 158–168 °C and 261–284 °C, respectively). The new types of WFO, studied for the first time, are suitable as a carbon substrates for PHA synthesis. The WFOs obtained in the production of canned Baltic sprat and Baltic mackerel can be considered a promising and renewable substrate for PHA biosynthesis.

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“…While marine and freshwater ecosystems are threatened by up to 23 million tons of plastics entering the oceans each year, the petrochemical plastics industry is thriving and plastic production could reach over 600 million tons produced by 2030 ( Borelle et al, 2020 ; MacLeod et al, 2021 ). To counter this threat, legislations must be enacted to curb plastic waste generation and promote the transition to more environmentally friendly yet competitive materials ( Gutschmann et al, 2022a ).…”

Section: Introductionmentioning

confidence: 99%

Tailoring the HHx monomer content of P(HB-co-HHx) by flexible substrate compositions: scale-up from deep-well-plates to laboratory bioreactor cultivations

Santolin

Thiele

Neubauer

et al. 2023

Front. Bioeng. Biotechnol.

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The enhanced material properties exhibited by the microbially synthetized polyhydroxyalkanoate (PHA) copolymer poly(hydroxybutyrate-co-hydroxyhexanoate) [P(HB-co-HHx)] evidence that this naturally biodegrading biopolymer could replace various functionalities of established petrochemical plastics. In fact, the thermal processability, toughness and degradation rate of P(HB-co-HHx) can be tuned by modulating its HHx molar content enabling to manufacture polymers à-la-carte. We have developed a simple batch strategy to precisely control the HHx content of P(HB-co-HHx) to obtain tailor-made PHAs with defined properties. By adjusting the ratio of fructose to canola oil as substrates for the cultivation of recombinant Ralstonia eutropha Re2058/pCB113, the molar fraction of HHx in P(HB-co-HHx) could be adjusted within a range of 2–17 mol% without compromising polymer yields. The chosen strategy proved to be robust from the mL-scale in deep-well-plates to 1-L batch bioreactor cultivations.

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Native feedstock options for the polyhydroxyalkanoate industry in Europe: A review

Cited by 24 publications

References 95 publications

Application of Immersed Membrane Bioreactor for Semi-Continuous Production of Polyhydroxyalkanoates from Organic Waste-Based Volatile Fatty Acids

Application of Immersed Membrane Bioreactor for Semi-Continuous Production of Polyhydroxyalkanoates from Organic Waste-Based Volatile Fatty Acids

Properties of Degradable Polyhydroxyalkanoates Synthesized from New Waste Fish Oils (WFOs)

Tailoring the HHx monomer content of P(HB-co-HHx) by flexible substrate compositions: scale-up from deep-well-plates to laboratory bioreactor cultivations

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