Native fluorescence spectra of human cancerous and normal breast tissues analyzed with non-negative constraint methods

Pu, Yang; Wang, Wubao; Yang, Yu; Alfano, R. R.

doi:10.1364/ao.52.001293

Cited by 56 publications

(32 citation statements)

References 20 publications

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“…The randomness in size and shape results in wider spatial frequency range in the higher grades in comparison with lower grade CIN and normal cervical tissues. Such features of disorder, aperiodicity, randomness, anti-symmetrical collagens in different sizes are also hallmarks of prostate [5,6], breast [7,8] and other types of dysplasia and/or tumor.…”

Section: Resultsmentioning

confidence: 99%

“…The sensitivity, specificity and the AUC values for using the extracted s of the spatial frequency combined with SVM for grading CIN tissues are summarized in Table I: It can seen from Table I that the good sensitivity, specificity, and AUC values demonstrate the excellent efficacy of the spatial frequency combined with SVM as a promising diagnostic tool for CIN detection [8]. These results outperform the current cervical cancer screening techniques, such as visual inspection using acetic acid (VIA), Genital human papillomavirus (HPV) Pap smear [11].…”

Section: Resultsmentioning

confidence: 99%

“…While the subjective nature of the histological evaluation is associated with considerable inter-and intra-observer variation in grading the biopsy tissues, our result obtained by spatial frequency spectra analysis is a direct function of the periodic, random, symmetrical, sized and orderly features of structure of the tissue and can therefore give an objective new picture of pathology. Furthermore, this study presents a potential criterion to diagnose early stage cervical tumor and other types of dysplasia having similar tissue structural changes [5][6][7][8] using the spatial frequencies.…”

Section: Resultsmentioning

confidence: 99%

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Optical quantitative pathology of cervical intraepithelial neoplasia in human tissues using spatial frequency analysis

Jagtap

Alfano

2014

Journal of Biophotonics

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An optical quantitative histological method in human tissues using spatial frequencies is demonstrated. Optical spatial frequency spectra from different stages of human Cervical Intraepithelial Neoplasia (CIN) tissue are evaluated as a potential quantitative pathological tool. The degree of randomness of tissue structures from normal to different stages of CIN tissue can be recognized by spatial frequency analysis. The standard deviation, σ of human normal and CIN tissue, is obtained by assuming the spatial frequency spectra as a Gaussian distribution. A support vector machine classifier (SVM) is trained in the subspace of σ. Twenty-eight normal and CIN samples of varying grades are examined and compared with current diagnostic outcomes. Our results suggest that an excellent accuracy for diagnostic purposes can be achieved. This approach offers a simple, efficient and objective way to supplement histopathology in recognizing alterations from normal to different stages of cervical pre-cancer, which are reflected by spatial information contained within the aperiodic and random structures of the different types of tissue.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Optical quantitative pathology of cervical intraepithelial neoplasia in human tissues using spatial frequency analysis

Jagtap

Alfano

2014

Journal of Biophotonics

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“…It is well-known that these optical fingerprints could be used as an alternative technique to detect cancer [1][2][3][4][5][6][7][8][9][10]. In our previous work, we examined the spectral fingerprints of tissues from patients with different breast cancer histologies using a novel fluorescence device known as the S 3 -LED ratiometer unit, and a LED excitation wavelength of 280 nm.…”

Section: Introductionmentioning

confidence: 99%

A comparison study of different excitation wavelengths to determine the relative content of key biomolecules in breast cancer and breast normal tissue

Sordillo

Budansky

et al. 2015

SPIE Proceedings

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Fluorescence profiles from breast cancer and breast normal tissue samples with excitation wavelengths at 280 nm and 340 nm were obtained using the conventional LS-50 Perkin-Elmer spectrometer. Fluorescence ratios from these tissue samples, demonstrated by emission peaks at 340 nm, 440 nm and 460 nm and likely representing tryptophan and NADH, show increased relative content of tryptophan in malignant samples. Double ratio (DR) techniques were used to measure the severity of disease. The single excitation double ratio (Single-DR) method utilizes the emission intensity peaks from the spectrum acquired using a single excitation of 280 nm; while the dual excitation double ratio (dual-DR) method utilizes the emission intensity peaks from the spectra acquired using an excitation of 280 nm and 340 nm. Single-DR and dual-DR from 13 patients with breast carcinoma were compared in terms of their efficiency to distinguish high from low/intermediate tumors. Similar results were found with both methods. Results suggest that dual excitation wavelengths may be as effective as single excitation wavelength in calculating the relative content of biomolecules in breast cancer tissue, as well as for the assessment of the malignant potential of these tumors.

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“…Many biological materials have photophysical properties that are based on their molecular structure and content of building blocks [8,9]. These photophysical properties, especially fl uorescence, have been considered as useful parameters to monitor changes in the tissues, which depend on the concentration of the fl uorophores and their excitation wavelengths [10][11][12].…”

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confidence: 99%

Influence of Tissue Fluorescence Measurement and Imaging by Auto-Fluorescence of Substrata

et al. 2015

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535.372 and R. S. Jayasree Polymers, glass and fi ber reinforced materials are increasingly being used in photophysical devices. The inherent fl uorescence of devices made of these materials may itself become important during fl uorescence detection. It may interfere with or even negate the low fl uorescence signals emitted from fl uorophores molecules of interest especially when the fl uorophores exist in microvolume samples. Such effects are signifi cant when tissue micro-sections placed on substrata of high background fl uorescence. Using different excitation wavelengths, fl uorescence spectral and imaging studies were carried out to examine the effect of background fl uorescence of microslides made of various substrata such as glass, polycarbonate and aluminium on autofl uorescence of tissue sections. With decreasing wavelength of excitation, all substrata showed increased autofl uorescence. Glass showed least inherent fl uorescence and aluminium showed more refl ectance than autofl uorescence. After continuous exposure to blue and UV light, all substrata showed irreversible decreased autofl uorescence. During spectrofl uorimetric studies, a defi nite blue shift was observed in the autofl uorescence of tissue sections placed on microslides made of each of the materials. This suggests an interference of tissue autofl uorescence by background fl uorescence emitted by the substratum. This may lead to false positive or negative reporting of the fl uorophores, particularly important while analyzing micro-sections of biological samples. Microscopic imaging did not show any background fl uorescence for glass substratum with blue light. However, with UV light, glass also showed background fl uorescence during imaging. Other substrata showed strong background fl uorescence or refl ectance with both blue and UV light. This study has the potential for accurate quantifi cation of fl uorescence spectral and decay and improved fl uorescence imaging using the photobleaching effect.Introduction. Polymers, plastics, and fi ber reinforced materials are increasingly being used in the micro fabricated fl uidic devices (μFFD), micro total analysis system (μTAS), and biomedical engineering with increased speed, reliability, and reduced sample consumption and cost [1][2][3]. Depending on different applications, relatively inexpensive and disposable devices can be made of polymers, fi bers, and plastic materials, compared to traditional materials like glass, quartz, and silicon [1,4,5]. Polymers and plastics are safer than glass and quartz slides as they are more impact resistant and do not produce any sharp edges when they break. However, the surface or interface properties of these materials may greatly affect the device performance especially during fl uorescence detection, as many of these materials have their own intrinsic auto-fl uorescence [6,7]. Hence, inherent fl uorescence of the substratum itself becomes important, since it may interfere with or even negate the low fl uorescence signals emitted from molecule of interest, ...

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Native fluorescence spectra of human cancerous and normal breast tissues analyzed with non-negative constraint methods

Cited by 56 publications

References 20 publications

Optical quantitative pathology of cervical intraepithelial neoplasia in human tissues using spatial frequency analysis

Optical quantitative pathology of cervical intraepithelial neoplasia in human tissues using spatial frequency analysis

A comparison study of different excitation wavelengths to determine the relative content of key biomolecules in breast cancer and breast normal tissue

Influence of Tissue Fluorescence Measurement and Imaging by Auto-Fluorescence of Substrata

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