Native microbiome impedes vertical transmission of
            <i>Wolbachia</i>
            in
            <i>Anopheles</i>
            mosquitoes

Hughes, Grant L.; Dodson, Brittany L.; Johnson, R. H.; Murdock, Courtney C.; Tsujimoto, Hitoshi; Suzuki, Yasutsugu; Patt, Alyssa A.; Cui, Long; Nossa, Carlos W.; Barry, Rhiannon; Sakamoto, Joyce M.; Hornett, Emily A.; Rasgon, Jason L.

doi:10.1073/pnas.1408888111

Cited by 231 publications

(247 citation statements)

References 63 publications

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“…Moreover, Caccia et al reported that mortality of the cotton leafworm Spodoptera littoralis was increased by the gut bacteria Serratia and Clostridia species invading the body cavity through toxin-induced epithelial lesions (31). In Anopheles, the symbiotic bacterium Asaia is responsible for inhibiting Wolbachia transmission but antibiotic microbiome perturbation enables Wolbachia transmission (34). Examples of the contributory role of gut microbiota to invading intestinal pathogens are also found among vertebrates.…”

Section: Discussionmentioning

confidence: 99%

Insect pathogenic fungus interacts with the gut microbiota to accelerate mosquito mortality

Lai

Wang

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

268

243

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The insect gut microbiota plays crucial roles in modulating the interactions between the host and intestinal pathogens. Unlike viruses, bacteria, and parasites, which need to be ingested to cause disease, entomopathogenic fungi infect insects through the cuticle and proliferate in the hemolymph. However, interactions between the gut microbiota and entomopathogenic fungi are unknown. Here we show that the pathogenic fungus Beauveria bassiana interacts with the gut microbiota to accelerate mosquito death. After topical fungal infection, mosquitoes with gut microbiota die significantly faster than mosquitoes without microbiota. Furthermore, fungal infection causes dysbiosis of mosquito gut microbiota with a significant increase in gut bacterial load and a significant decrease in bacterial diversity. In particular, the opportunistic pathogenic bacterium Serratia marcescens overgrows in the midgut and translocates to the hemocoel, which promotes fungal killing of mosquitoes. We further reveal that fungal infection down-regulates antimicrobial peptide and dual oxidase expression in the midgut. Duox down-regulation in the midgut is mediated by secretion of the toxin oosporein from B. bassiana. Our findings reveal the important contribution of the gut microbiota in B. bassiana-killing activity, providing new insights into the mechanisms of fungal pathogenesis in insects.

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Section: Discussionmentioning

confidence: 99%

Insect pathogenic fungus interacts with the gut microbiota to accelerate mosquito mortality

Lai

Wang

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

268

243

View full text Add to dashboard Cite

show abstract

“…Unfortunately, while studies on the application of microbes such as Wolbachia to control human disease have advanced, we still lack basic insight into the natural microbial communities associated with vectors, from viruses to bacteria to single‐celled eukaryotes. Given that infectious vector‐borne diseases are increasing in humans (Jones et al., 2008), our goal is to characterize the composition and structure of microbiota in mosquito vectors across landscapes altered by human activities, where differences may influence the biology of vectors, their vectorial capacity, and ultimately the outcome of microbe‐mediated disease control (Hughes et al., 2014). …”

Section: Introductionmentioning

confidence: 99%

Mosquito vector‐associated microbiota: Metabarcoding bacteria and eukaryotic symbionts across habitat types in Thailand endemic for dengue and other arthropod‐borne diseases

Thongsripong

Chandler

Green

et al. 2017

Ecology and Evolution

124

112

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Vector‐borne diseases are a major health burden, yet factors affecting their spread are only partially understood. For example, microbial symbionts can impact mosquito reproduction, survival, and vectorial capacity, and hence affect disease transmission. Nonetheless, current knowledge of mosquito‐associated microbial communities is limited. To characterize the bacterial and eukaryotic microbial communities of multiple vector species collected from different habitat types in disease endemic areas, we employed next‐generation 454 pyrosequencing of 16S and 18S rRNA amplicon libraries, also known as metabarcoding. We investigated pooled whole adult mosquitoes of three medically important vectors, Aedes aegypti, Ae. albopictus, and Culex quinquefasciatus, collected from different habitats across central Thailand where we previously characterized mosquito diversity. Our results indicate that diversity within the mosquito microbiota is low, with the majority of microbes assigned to one or a few taxa. Two of the most common eukaryotic and bacterial genera recovered (Ascogregarina and Wolbachia, respectively) are known mosquito endosymbionts with potentially parasitic and long evolutionary relationships with their hosts. Patterns of microbial composition and diversity appeared to differ by both vector species and habitat for a given species, although high variability between samples suggests a strong stochastic element to microbiota assembly. In general, our findings suggest that multiple factors, such as habitat condition and mosquito species identity, may influence overall microbial community composition, and thus provide a basis for further investigations into the interactions between vectors, their microbial communities, and human‐impacted landscapes that may ultimately affect vector‐borne disease risk.

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“…Further, the microbiomes of arthropod vectors of human pathogens can affect the transmission of zoonotic pathogens (Weiss and Aksoy, 2011;Hughes et al, 2014;Narasimhan et al, 2014). Laboratory experiments have found that higher tick microbiome diversity is negatively correlated with colonization success of the Lyme disease pathogen, Borrelia burgdorferi (Narasimhan et al, 2014).…”

mentioning

confidence: 99%

Tick microbiome and pathogen acquisition altered by host blood meal

2016

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Lyme disease, a zoonotic disease, is the most prevalent vector-borne disease in the Northern Hemisphere. Diversity of the vector (tick) microbiome can impact pathogen transmission, yet the biotic and abiotic factors that drive microbiome diversity are largely unresolved, especially under natural, field conditions. We describe the microbiome of Ixodes pacificus ticks, the vector for Lyme disease in the western United States, and show a strong impact of host blood meal identity on tick microbiome species richness and composition. Western fence lizards, a host that is refractory to the Lyme disease pathogen, significantly reduces microbiome diversity in ticks relative to ticks that feed on a mammalian reservoir host. Host blood meal-driven reduction of tick microbiome diversity may have lifelong repercussions on I. pacificus vector competency and ultimately disease dynamics.

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Native microbiome impedes vertical transmission of Wolbachia in Anopheles mosquitoes

Cited by 231 publications

References 63 publications

Insect pathogenic fungus interacts with the gut microbiota to accelerate mosquito mortality

Insect pathogenic fungus interacts with the gut microbiota to accelerate mosquito mortality

Mosquito vector‐associated microbiota: Metabarcoding bacteria and eukaryotic symbionts across habitat types in Thailand endemic for dengue and other arthropod‐borne diseases

Tick microbiome and pathogen acquisition altered by host blood meal

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