Native promoter-mediated transcriptional regulation of crucial oleosin protein OLE1 from Prunus sibirica for seed development and high oil accumulation

Hu, Jinhe; Chen, Feng; Zang, Junxin; Li, Zhi; Wang, Jing; Wang, Zirui; Shi, Lingling; Xiu, Yu; Lin, Shanzhi

doi:10.1016/j.ijbiomac.2023.126650

Cited by 7 publications

(6 citation statements)

References 60 publications

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“…6). Ole1 overexpression has been shown to promote oil accumulation by inhibiting TAG degradation-associated transport proteins and lipases, and by activating enzymes related to TAG assembly (Zhang et al, 2019; Xu et al, 2020; Hu et al, 2023). TAG is one of the ultimate products for the Kennedy pathway.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptomic and lipidomic analysis of fatty acid accumulation in threeCamellia oleiferavarieties during maturation

Yang,

Wang,

Lai

et al. 2024

Preprint

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Camellia oleiferastands out as one of China’s leading woody oil crops, famed for its tea oil, which boasts a high content of unsaturated fatty acids. Often referred to as "liquid gold" or "Oriental olive oil," this oil is prized for its nutritional benefits and culinary versatility, marking Camellia oleifera as a valuable agricultural and economic resource. This study provides an integrated investigation of the transcriptome and lipidome within seeds at the maturation stage across threeC. oleiferavarieties, revealing a significant relationship between fatty acid production and genes involved in lipid synthesis. Through transcriptomic analysis, 26,344 genes with varied expression were found. Functional enrichment analysis highlighted that pathways related to starch and sucrose metabolism, plant hormone signal transduction, and lipid accumulation were highly enriched among the differentially expressed genes. Additionally, expression variations ofSADandFADsamong different varieties were explored. The analysis suggests that increased expression of genes such asFAD3,FAD7, andFAD8during the maturation stage may enhance the proportion of linolenic acid while reducing oleic acid content. This alteration could potentially stimulate fatty acid synthesis through a feedback regulation mechanism, thereby enhancing seed oil content. These findings provide a novel perspective on the feedback regulation mechanisms underlying lipid biosynthesis inC. oleiferaseeds, offering significant insights to boost oil yield and improve tea oil quality.

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Section: Discussionmentioning

confidence: 99%

Comparative transcriptomic and lipidomic analysis of fatty acid accumulation in threeCamellia oleiferavarieties during maturation

Yang,

Wang,

Lai

et al. 2024

Preprint

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“…The importance of oleosins in LD formation and stabilization has prompted active research in oil crops [ 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 ]. Nevertheless, despite the proposal of five oleosin clades (i.e., U, SL, SH, M, and T) in angiosperms [ 4 , 8 ], their evolution in eudicots has not been well-established.…”

Section: Discussionmentioning

confidence: 99%

Integrative Analysis of Oleosin Genes Provides Insights into Lineage-Specific Family Evolution in Brassicales

Zou,

Zhang,

Zhao

2024

Plants

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Oleosins (OLEs) are a class of small but abundant structural proteins that play essential roles in the formation and stabilization of lipid droplets (LDs) in seeds of oil crops. Despite the proposal of five oleosin clades (i.e., U, SL, SH, T, and M) in angiosperms, their evolution in eudicots has not been well-established. In this study, we employed Brassicales, an economically important order of flowering plants possessing the lineage-specific T clade, as an example to address this issue. Three to 10 members were identified from 10 species representing eight plant families, which include Caricaceae, Moringaceae, Akaniaceae, Capparaceae, and Cleomaceae. Evolutionary and reciprocal best hit-based homologous analyses assigned 98 oleosin genes into six clades (i.e., U, SL, SH, M, N, and T) and nine orthogroups (i.e., U1, U2, SL, SH1, SH2, SH3, M, N, and T). The newly identified N clade represents an ancient group that has already appeared in the basal angiosperm Amborella trichopoda, which are constitutively expressed in the tree fruit crop Carica papaya, including pulp and seeds of the fruit. Moreover, similar to Clade N, the previously defined M clade is actually not Lauraceae-specific but an ancient and widely distributed group that diverged before the radiation of angiosperm. Compared with A. trichopoda, lineage-specific expansion of the family in Brassicales was largely contributed by recent whole-genome duplications (WGDs) as well as the ancient γ event shared by all core eudicots. In contrast to the flower-preferential expression of Clade T, transcript profiling revealed an apparent seed/embryo/endosperm-predominant expression pattern of most oleosin genes in Arabidopsis thaliana and C. papaya. Moreover, the structure and expression divergence of paralogous pairs was frequently observed, and a good example is the lineage-specific gain of an intron. These findings provide insights into lineage-specific family evolution in Brassicales, which facilitates further functional studies in nonmodel plants such as C. papaya.

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“…In oilseed plants, oil accumulation is known as a part of seed development, involving a series of gene expression. High transcripts of the enzymes required for FA biosynthesis and TAG assembly may play a key contribution to oil accumulation in the seeds of several oil plants [ 18 , 52 – 54 , 62 , 64 ]. Here, the enzymes relevant for FA synthesis (EAR, KAS I/II/III, ACC, SAD6, FATA/B, MAT, and FAD2) and TAG assembly (LACS9, PAP2, GPAT9, LPAAT4, and DGAT1) were coordinated transcriptionally with PsMDL2 in the seeds of PsMDL2 transgenic lines (Figs.…”

Section: Discussionmentioning

confidence: 99%

“…Transient expression analysis of fusion protein PsMDL2-GFP was applied to detect subcellular location of PsMDL2 in leaf cells of Nicotiana benthamiana [ 17 , 18 ]. The complete ORF of PsMDL2 was cloned into pBI121/ 35S::GFP to generate vector of 35S::PsMDL2 - GFP (Additional file 7: Fig.…”

Section: Methodsmentioning

confidence: 99%

“…The total area was approximately 1.7 million hectares, yielding around 192,500 metric tons of the seeds each year [ 14 – 19 ]. The mature dry seeds of P. sibirica contained a substantial amount of the oils (44.7−58.7%) with a high proportion of linoleic acid (16.4−34.7%) and oleic acid (56.2−76.3%) [ 13 , 14 , 18 , 20 ], which has been applied in various fields including medicinal product, cosmetic, surfactant, edible oil and biodiesel feedstock [ 14 – 17 , 21 ]. Also, the seeds show a potential usefulness in human nutrition and traditional Chinese medicine [ 22 – 26 ].…”

Section: Introductionmentioning

confidence: 99%

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Mandelonitrile lyase MDL2-mediated regulation of seed amygdalin and oil accumulation of Prunus Sibirica

Chen,

Zang,

Wang

et al. 2024

BMC Plant Biol

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Background The Prunus sibirica seeds with rich oils has great utilization, but contain amygdalin that can be hydrolyzed to release toxic HCN. Thus, how to effectively reduce seed amygdalin content of P. sibirica is an interesting question. Mandelonitrile is known as one key intermediate of amygdalin metabolism, but which mandelonitrile lyase (MDL) family member essential for its dissociation destined to low amygdalin accumulation in P. sibirica seeds still remains enigmatic. An integration of our recent 454 RNA-seq data, amygdalin and mandelonitrile content detection, qRT-PCR analysis and function determination is described as a critical attempt to determine key MDL and to highlight its function in governing mandelonitrile catabolism with low amygdalin accumulation in Prunus sibirica seeds for better developing edible oil and biodiesel in China. Results To identify key MDL and to unravel its function in governing seed mandelonitrile catabolism with low amygdalin accumulation in P. sibirica. Global identification of mandelonitrile catabolism-associated MDLs, integrated with the across-accessions/developing stages association of accumulative amount of amygdalin and mandelonitrile with transcriptional level of MDLs was performed on P. sibirica seeds of 5 accessions to determine crucial MDL2 for seed mandelonitrile catabolism of P. sibirica. MDL2 gene was cloned from the seeds of P. sibirica, and yeast eukaryotic expression revealed an ability of MDL2 to specifically catalyze the dissociation of mandelonitrile with the ideal values of Km (0.22 mM) and Vmax (178.57 U/mg). A combination of overexpression and mutation was conducted in Arabidopsis. Overexpression of PsMDL2 decreased seed mandelonitrile content with an increase of oil accumulation, upregulated transcript of mandelonitrile metabolic enzymes and oil synthesis enzymes (involving FA biosynthesis and TAG assembly), but exhibited an opposite situation in mdl2 mutant, revealing a role of PsMDL2-mediated regulation in seed amygdalin and oil biosynthesis. The PsMDL2 gene has shown as key molecular target for bioengineering high seed oil production with low amygdalin in oilseed plants. Conclusions This work presents the first integrated assay of genome-wide identification of mandelonitrile catabolism-related MDLs and the comparative association of transcriptional level of MDLs with accumulative amount of amygdalin and mandelonitrile in the seeds across different germplasms and developmental periods of P. sibirica to determine MDL2 for mandelonitrile dissociation, and an effective combination of PsMDL2 expression and mutation, oil and mandelonitrile content detection and qRT-PCR assay was performed to unravel a mechanism of PsMDL2 for controlling amygdalin and oil production in P. sibirica seeds. These findings could offer new bioengineering strategy for high oil production with low amygdalin in oil plants.

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Native promoter-mediated transcriptional regulation of crucial oleosin protein OLE1 from Prunus sibirica for seed development and high oil accumulation

Cited by 7 publications

References 60 publications

Comparative transcriptomic and lipidomic analysis of fatty acid accumulation in threeCamellia oleiferavarieties during maturation

Comparative transcriptomic and lipidomic analysis of fatty acid accumulation in threeCamellia oleiferavarieties during maturation

Integrative Analysis of Oleosin Genes Provides Insights into Lineage-Specific Family Evolution in Brassicales

Mandelonitrile lyase MDL2-mediated regulation of seed amygdalin and oil accumulation of Prunus Sibirica

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