Native Salivary Chromosomes of Drosophila melanogaster: Retrospect and Prospect

Hill, Ronald J.

doi:10.1071/bi9850001

Cited by 6 publications

(3 citation statements)

References 28 publications

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“… 34 , 35 Noteworthy, through immunofluorescence analysis it has been also reported that acetic acid fixation leads to the transition to Z-conformation in chromosomes, as a result of torsional stress in DNA. 36 …”

Section: Resultsmentioning

confidence: 99%

Role of water in chromosome spreading and swelling induced by acetic acid treatment: a FTIR spectroscopy study

et al. 2014

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The so called chromosome preparation is a procedure consisting of three strictly connected stages that enables to obtain chromosomes of quality suitable for cytogenetic analysis. Interestingly, experimental evidence strongly suggested that chromosome spreading and swelling (key processes that allow their counting and detailed structural analysis) are induced in the last fixative-evaporation stage by the interaction, mediated by acetic acid, between water from the environmental humidity, and the cytoplasmic matrix and the chromatin. However, since a considerable variation in the quality of chromosome preparations is observed, strongly depending on the environmental conditions in which the procedure takes place, a better comprehension of the mechanisms underlying chromosome preparation is required. To this aim, here we analysed intact lymphocytes before and at each stage of the chromosome preparation protocol by Fourier transform infrared (FTIR) spectroscopy, a technique widely used for the study not only of isolated biomolecules, but also of complex biological systems, such as whole cells. Interestingly, we found that the chromosome preparation protocol induces significant structural changes of cell proteins and DNA, in particular due to the interaction with acetic acid. Moreover, noteworthy, through the monitoring of changes in the water combination band between 2300 and 1800 cm–1, we provided evidence at molecular level of the crucial role of the bound water to the cytoplasmic matrix and to the chromatin in determining the chromosome spreading and swelling. Our FTIR results, therefore, underline the need to perform the last fixative-evaporation stage in standardized and optimized temperature and relative humidity conditions, thus providing chromosomes of high quality for the cytogenetic analysis that would lead in this way to more reliable results.

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Section: Resultsmentioning

confidence: 99%

Role of water in chromosome spreading and swelling induced by acetic acid treatment: a FTIR spectroscopy study

et al. 2014

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“…The influence of acid fixatives on chromosome structure has been described [8,26]. It seems that the appearance of doublet structures may be due to vacuolization of compact bands, probably due to structural changes in chromatin during fixation [26].…”

Section: Discussionmentioning

confidence: 99%

Electron microscope analysis of the E polytene chromosome of Drosophila subobscura: region 70B to 72D

Pérez-Alonso

Cuenca

Frutos

1988

Biology of the Cell

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Revision of the reference map of the polytene chromosomes of Drosophila subobscura was started by means of the electron microscope. We present a map of regions 70B to 72D of the E chromosome obtained from squashed and thin-sectioned salivary glands. It was observed that the total number of bands in divisions 70B to 72D is considerably higher than those depicted in the reference map of Kunze-Mühl and Müller. Functional considerations are made of some regions that show puff structures.

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Section: Discussionmentioning

confidence: 99%

Electron microscope analysis of the E polytene chromosome of Drosophila subobscura: region 70B to 72D

PEREZALONSO¹

1988

Biology of the Cell

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Native Salivary Chromosomes of Drosophila melanogaster: Retrospect and Prospect

Cited by 6 publications

References 28 publications

Role of water in chromosome spreading and swelling induced by acetic acid treatment: a FTIR spectroscopy study

Role of water in chromosome spreading and swelling induced by acetic acid treatment: a FTIR spectroscopy study

Electron microscope analysis of the E polytene chromosome of Drosophila subobscura: region 70B to 72D

Electron microscope analysis of the E polytene chromosome of Drosophila subobscura: region 70B to 72D

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