2011
DOI: 10.1074/jbc.m111.248641
|View full text |Cite
|
Sign up to set email alerts
|

Native Thrombocidin-1 and Unfolded Thrombocidin-1 Exert Antimicrobial Activity via Distinct Structural Elements

Abstract: Background:The properties required for antimicrobial activity of chemokines are unclear. Results: Native thrombocidin-1 requires a three-dimensional positive patch for activity, but unfolded thrombocidin-1 is active through the N-terminal linear peptide regions. Conclusion: Native thrombocidin-1 and unfolded thrombocidin-1 exert activity via distinct structural elements. Significance: Folded and unfolded antimicrobial chemokines can exert activity through different structural elements.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

2
27
0

Year Published

2012
2012
2018
2018

Publication Types

Select...
3
3
1

Relationship

0
7

Authors

Journals

citations
Cited by 37 publications
(29 citation statements)
references
References 51 publications
2
27
0
Order By: Relevance
“…Intact CXCL14, however, is also quite potent in killing Gram-positive species, and this effect was evident in vivo indicating that the tertiary structure of CXCL14 could be responsible for interaction with the cell wall of Gram-positive bacteria. The notion that different mechanisms could be responsible for the killing of Gram-positive and Gram-negative bacteria is consistent with a recent study where N-terminal peptides of thrombocidin-1 were compared with the full-size molecule and found to be less active against Gram-positive S. aureus, but equally efficient with E. coli (26). Accordingly, it is logical to assume that the composition of the linear peptide stretch is important in short peptides where three-dimensional structural elements such as positive patches are less likely to occur.…”
Section: Discussionsupporting
confidence: 72%
See 2 more Smart Citations
“…Intact CXCL14, however, is also quite potent in killing Gram-positive species, and this effect was evident in vivo indicating that the tertiary structure of CXCL14 could be responsible for interaction with the cell wall of Gram-positive bacteria. The notion that different mechanisms could be responsible for the killing of Gram-positive and Gram-negative bacteria is consistent with a recent study where N-terminal peptides of thrombocidin-1 were compared with the full-size molecule and found to be less active against Gram-positive S. aureus, but equally efficient with E. coli (26). Accordingly, it is logical to assume that the composition of the linear peptide stretch is important in short peptides where three-dimensional structural elements such as positive patches are less likely to occur.…”
Section: Discussionsupporting
confidence: 72%
“…This finding was unexpected because positively charged a-helices were considered to be an epitome for a chemokine with AMP function (7,8,24,40). Nevertheless, CXCL6 and thrombocidin-1, a truncated form of CXCL7, are other examples of antimicrobial chemokines where the microbicidal effect is mediated by 50-and 15-aa-long N-terminal peptides, respectively (25,26,46). Moreover, similar to other chemokines and defensins, CXCL14 displayed antimicrobial activity after linearization (7,27,47).…”
Section: Discussionmentioning
confidence: 92%
See 1 more Smart Citation
“…α granules also contain proteins that exhibit anti-microbial effects and thus influence innate immune function. Examples include thrombocidin 1 and 2, which have anti-bacterial and anti-fungal actions (115). α granules also contain proteins that possess mitogenic and angiogenic abilities and thus regulate wound healing and angiogenesis function.…”
Section: Overview Of Plateletsmentioning
confidence: 99%
“…Beside tPMP, the thrombocidins TC1 and TC2 also have the potency to inhibit the growth of bacteria in vitro, such as E. coli, Bacillus subtilis and S. aureus [51,114]. The IE model confirmed these results in vivo, demonstrating that TC1 and TC2 are involved in the clearance of Streptococcus viridans from the endocardial surface; bacterial strains with low TC1/TC2 susceptibility could persist on the surface, whereas highly susceptible bacteria were rapidly eliminated [115,116].…”
Section: Platelets' Benefit In Bacterial Infection: Direct Attack and Gmentioning
confidence: 50%