Natrinema caseinilyticum sp. nov., Natrinema gelatinilyticum sp. nov., Natrinema marinum sp. nov., Natrinema zhouii sp. nov., extremely halophilic archaea isolated from marine environments and a salt mine

Hu, Yue; Xue, Ming; Li, Xinxin; Tan, Shun; Cheng, Ming; Hou, Jing; Cui, Heng‐Lin

doi:10.1007/s00792-023-01294-3

Cited by 5 publications

(4 citation statements)

References 57 publications

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“…Strains XZGYJ-43 T and ZJ1 T were isolated from saline soil of Mangkang ancient solar saltern (Tibet, PR China) and the offshore sediment of Zhujiang river inlet (Guangdong, PR China), respectively [8,9]. The neutral haloarchaeal medium (NHM) was used to cultivate halophilic archaea [10].…”

Section: Cultivation and Isolation Of Halophilic Archaeamentioning

confidence: 99%

“…The genomes of the current Haladaptatus species range from 3.9 to 7.3 Mbp and their G+C contents are 56.1-62.1 mol% [3][4][5][6][7]. In the previous investigation of the halophilic archaeal diversity of hypersaline environments in PR China, halophilic archaeal strains XZGYJ-43 T and ZJ1 T were isolated from Mangkang ancient solar saltern (Tibet) and Zhujiang river inlet (Guangdong), respectively [8,9]. Strain XZGYJ-43 T was found to be most related to the current genera of the family Halobacteriaceae and strain ZJ1 T was closely related to the current species of genus Haladaptatus judged by 16S rRNA gene similarities, respectively.…”

Section: Introductionmentioning

confidence: 99%

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Halospeciosus flavus gen. nov., sp. nov. and Haladaptatus caseinilyticus sp. nov., halophilic archaea isolated from saline soil of an inland solar saltern and offshore sediment

Li,

Tan,

Cheng

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

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Two novel halophilic archaeal strains (XZGYJ-43T and ZJ1T) were isolated from Mangkang ancient solar saltern (Tibet, PR China) and Zhujiang river inlet (Guangdong, PR China), respectively. The comparison of the 16S rRNA gene sequences revealed that strain XZGYJ-43T is related to the current species of the family Halobacteriaceae (89.2–91.7% similarity) and strain ZJ1T showed 94.7–98.3% similarity to the current species of the genus Haladaptatus . Phylogenetic analyses based on 16S rRNA genes, rpoB′ genes and genomes indicated that strain XZGYJ-43T is separate from the related genera, Halocalculus , Salarchaeum and Halarchaeum of the family Halobacteriaceae , and strain ZJ1T tightly clusters with the current species of the genus Haladaptatus . The average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity values between strain XZGYJ-43T and the current species of the family Halobacteriaceae were 71–75, 20–25 and 59–68 %, and these values between strain ZJ1T and the current species of the genus Haladaptatus were 77–81, 27–32 and 76–82 %, respectively, clearly below the thresholds for prokaryotic species demarcation. These two strains could be distinguished from their relatives according to differential phenotypic characteristics. The major polar lipids of strain XZGYJ-43T were phosphatidic acid (PA), phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), mannosyl glucosyl diether (DGD-1; DGD-PA) and sulphated mannosyl glucosyl diether (S-DGD-1; S-DGD-PA), and those of strain ZJ1T were PA, PG, PGP-Me, DGD-PA, S-DGD-1 (S-DGD-PA) and sulphated galactosyl mannosyl glucosyl diether. Based on phenotypic, phylogenetic and genomic data, strain XZGYJ-43T (=CGMCC 1.13890T=JCM 33735T) represents a novel species of a new genus within the family Halobacteriaceae , and strain ZJ1T (=CGMCC 1.18785T=JCM 34917T) represents a novel species of the genus Haladaptatus , for which the names Halospeciosus flavus gen. nov., sp. nov. and Haladaptatus caseinilyticus sp. nov. are proposed, respectively.

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Section: Cultivation and Isolation Of Halophilic Archaeamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Halospeciosus flavus gen. nov., sp. nov. and Haladaptatus caseinilyticus sp. nov., halophilic archaea isolated from saline soil of an inland solar saltern and offshore sediment

Li,

Tan,

Cheng

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

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“…They were unable to utilize d-fructose, l-sorbose, d-ribose, d-xylose, maltose, lactose, glycine, l-alanine or l-lysine. These two strains were sensitive to the following antimicrobial compounds (µg per disc, unless otherwise indicated): novobiocin (30) and nitrofurantoin (300); but resistant to trimethoprim (5), penicillin G (10 IU per disc), ampicillin (10), chloramphenicol (30), neomycin (30), norfloxacin (10), ciprofloxacin (5), streptomycin (10), kanamycin (30), vancomycin (30), gentamicin (10) and nalidixic acid (30). More detailed features of strains GSLN9 T and XZYJT29 T are given in the species descriptions.…”

Section: Morphological and Physiological Characteristicsmentioning

confidence: 99%

“…Functional annotation of these genomes was performed through the online Rapid Annotation using Subsystem Technology (rast) server [29]. The identification and exploration of functional genes with biotechnological potential were conducted as described previously [17,30]. The orthologous clusters (OCs) between strains GSLN9 T , XZYJT29 T and the closely related species were compared and analysed using the OrthoVenn2 online tool [31].…”

Section: Genome Sequence Analysismentioning

confidence: 99%

Halomicrococcus gelatinilyticus sp. nov. and Halosimplex aquaticum sp. nov., halophilic archaea isolated from saline soil and an inland solar saltern

Hu,

Ma,

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

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Two extremely halophilic archaeal strains, GSLN9T and XZYJT29T, were isolated from the saline soil in different regions of western China. Both strains GSLN9T and XZYJT29T have two 16S rRNA genes with similarities of 95.1 and 94.8 %, respectively. Strain GSLN9T was mostly related to the genus Halomicrococcus based on 16S rRNA (showing 91.0–96.0 % identities) and rpoB′ genes (showing 92.0 % identity). Strain XZYJT29T showed 92.1–97.6 % (16S rRNA gene) and 91.4–93.1 % (rpoB′ gene) sequence similarities to its relatives in the genus Halosimplex , respectively. The polar lipid profile of strain GSLN9T included phosphatidic acid (PA), phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), phosphatidylglycerol sulphate (PGS), sulphated mannosyl glucosyl diether (S-DGD-1) and sulphated galactosyl mannosyl glucosyl diether (S-TGD-1), mostly similar to that of Halomicrococcus hydrotolerans H22T. PA, PG, PGP-Me, S-DGD-1 (S-DGD-PA), S2-DGD, S-TGD-1 and an unidentified glycolipid were detected in strain XZYJT29T; this polar lipid composition is similar to those of members of the genus Halosimplex . The average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity values between these two strains and their relatives of the genera Halomicrococcus and Halosimplex were no more than 82, 27 and 80 %, respectively, much lower than the thresholds for species demarcation. Other phenotypic characterization results indicated that strains GSLN9T and XZYJT29T can be differentiated from the current species of the genera Halomicrococcus and Halosimplex , respectively. These results revealed that strains GSLN9T (=CGMCC 1.15215T=JCM 30842T) and XZYJT29T (=CGMCC 1.15828T=JCM 31853T) represent novel species of Halomicrococcus and Halosimplex , for which the names Halomicrococcus gelatinilyticus sp. nov. and Halosimplex aquaticum sp. nov. are proposed.

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Genome-based classification of genera Halosegnis and Salella, and description of four novel halophilic archaea isolated from a tidal flat

Hu,

Ma,

Tan

et al. 2024

Antonie van Leeuwenhoek

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Natrinema caseinilyticum sp. nov., Natrinema gelatinilyticum sp. nov., Natrinema marinum sp. nov., Natrinema zhouii sp. nov., extremely halophilic archaea isolated from marine environments and a salt mine

Cited by 5 publications

References 57 publications

Halospeciosus flavus gen. nov., sp. nov. and Haladaptatus caseinilyticus sp. nov., halophilic archaea isolated from saline soil of an inland solar saltern and offshore sediment

Halospeciosus flavus gen. nov., sp. nov. and Haladaptatus caseinilyticus sp. nov., halophilic archaea isolated from saline soil of an inland solar saltern and offshore sediment

Halomicrococcus gelatinilyticus sp. nov. and Halosimplex aquaticum sp. nov., halophilic archaea isolated from saline soil and an inland solar saltern

Genome-based classification of genera Halosegnis and Salella, and description of four novel halophilic archaea isolated from a tidal flat

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