Natriuretic peptide-sensitive guanylyl cyclase expression is down-regulated in human melanoma cells at simulated weightlessness

Ivanova, Krassimira; Eiermann, Peter; Tsiockas, Wasiliki; Hauslage, Jens; Hemmersbach, Ruth; Gerzer, R.

doi:10.1016/j.actaastro.2010.08.002

Cited by 14 publications

(16 citation statements)

References 9 publications

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“…After exposed to MMG for 24, 48 or 72 h, U87 cells invaded and migrated more slowly than NG group, and it was in time-dependence to some degree, indicating MMG might inhibit the invasion and migration potentials of U87 cells. These results were in consistent with previous studies in other tumor cells, such as melanomas [15], human breast cancer [16] and follicular thyroid carcinoma cells [9]. Jing Li et al found that microgravity had closely associated with changing focal adhesion and cytoskeleton, and decreasing migration potential in human breast carcinoma [7].…”

Section: Discussionsupporting

confidence: 92%

Modeled microgravity suppressed invasion and migration of human glioblastoma U87 cells through downregulating store-operated calcium entry

Shi

Rao

Wang

et al. 2015

Biochemical and Biophysical Research Communications

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Section: Discussionsupporting

confidence: 92%

Modeled microgravity suppressed invasion and migration of human glioblastoma U87 cells through downregulating store-operated calcium entry

Shi

Rao

Wang

et al. 2015

Biochemical and Biophysical Research Communications

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“…Another paper reported that human melanoma cells showed a down-regulated expression of the membrane-bound guanylyl cyclases (GC) A and B at simulated weightlessness (fast-rotating clinostat). The authors suggested an attenuation of the metastatic potential in weightlessness because GC-A and GC-B expressions in human melanoma cells have been associated with metastasis [125]. These findings indicate that changes in expression, especially of membrane proteins, determine alteration of cellular behavior in microgravity.…”

Section: The Impact Of Weightlessness On Tumor Cellsmentioning

confidence: 95%

The Effects of Weightlessness on the Human Organism and Mammalian Cells

Pietsch¹,

Bauer²,

Egli

et al. 2011

CMM

131

110

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It has always been a desire of mankind to conquest Space. A major step in realizing this dream was the completion of the International Space Station (ISS). Living there for several months confirmed early observations of short-term spaceflights that a loss of gravity affects the health of astronauts. Space medicine tries to understand the mechanism of microgravity-induced health problems and to conceive potent countermeasures. There are four different aspects which make space medicine appealing: i) finding better strategies for adapting astronauts to weightlessness; ii) identification of microgravity-induced diseases (e.g. osteoporosis, muscle atrophy, cardiac problems and others); iii) defining new therapies to conquer these diseases which will benefit astronauts as well as people on Earth in the end; and iv) on top of that, unveiling the mechanisms of weightlessness-dependent molecular and cellular changes is a requirement for improving space medicine. In mammalian cells, microgravity induces apoptosis and alters the cytoskeleton and affects signal transduction pathways, cell differentiation, growth, proliferation, migration and adhesion. This review focused on gravi-sensitive signal transduction elements and pathways as well as molecular mechanisms in human cells, aiming to understand the cellular changes in altered gravity. Moreover, the latest information on how these changes lead to clinically relevant health problems and current strategies of countermeasures are reviewed.

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“…In cells grown in space, various changes have been observed, including cytoskeleton rearrangements, altered gene expression, and changes in cell signaling [78,79]. As the GC-cGMP pathway is involved in the melanocyte response to environmental stress and has also been linked to many cellular processes, including melanoma growth and migration, we compared the regulation of the GC-cGMP signaling pathway in a panel of human melanocytes and melanoma cell lines with different metastatic potential and pigmentation in hypergravity as well as under simulated microgravity conditions [80][81][82]. We used a specific centrifuge for the hypergravity experiments (up to 10 g) [80] and a fast-rotating 2D clinostat (60 rpm) as well as a fast-rotating clinostat microscope (60 rpm) to simulate microgravity conditions [81,83].…”

Section: Differential Effects Of Altered Gravity On Guanylyl Cyclase-mentioning

confidence: 99%

Guanylyl Cyclase-cGMP Signaling Pathway in Melanocytes: Differential Effects of Altered Gravity in Non-Metastatic and Metastatic Cells

Ivanova

Hemmersbach

2020

IJMS

Self Cite

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Human epidermal melanocytes as melanin producing skin cells represent a crucial barrier against UV-radiation and oxidative stress. It was shown that the intracellular signaling molecule cyclic guanosine-3 ,5 -monophosphate (cGMP), generated by the guanylyl cyclases (GCs), e.g., the nitric oxide (NO)-sensitive soluble GC (sGC) and the natriuretic peptide-activated particulate GC (GC-A/GC-B), plays a role in the melanocyte response to environmental stress. Importantly, cGMP is involved in NO-induced perturbation of melanocyte-extracellular matrix interactions and in addition, increased NO production during inflammation may lead to loss of melanocytes and support melanoma metastasis. Further, the NO-sensitive sGC is expressed predominantly in human melanocytes and non-metastatic melanoma cells, whereas absence of functional sGC but up-regulated expression of GC-A/GC-B and inducible NO synthase (iNOS) are detected in metastatic cells. Thus, suppression of sGC expression as well as up-regulated expression of GC-A/GC-B/iNOS appears to correlate with tumor aggressiveness. As the cGMP pathway plays important roles in melanocyte (patho)physiology, we present an overview on the differential effects of altered gravity (hypergravity/simulated microgravity) on the cGMP signaling pathway in melanocytes and melanoma cells with different metastatic potential. We believe that future experiments in real microgravity may benefit from considering cGMP signaling as a possible factor for melanocyte transformation and in medication.

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Natriuretic peptide-sensitive guanylyl cyclase expression is down-regulated in human melanoma cells at simulated weightlessness

Cited by 14 publications

References 9 publications

Modeled microgravity suppressed invasion and migration of human glioblastoma U87 cells through downregulating store-operated calcium entry

Modeled microgravity suppressed invasion and migration of human glioblastoma U87 cells through downregulating store-operated calcium entry

The Effects of Weightlessness on the Human Organism and Mammalian Cells

Guanylyl Cyclase-cGMP Signaling Pathway in Melanocytes: Differential Effects of Altered Gravity in Non-Metastatic and Metastatic Cells

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