Natronobacterium texcoconense sp. nov., a haloalkaliphilic archaeon isolated from soil of a former lake

Ruiz-Romero, Erick; Sánchez-López, Katia Berenice; Coutiño-Coutiño, María de los Angeles; González-Pozos, Sirenia; Bello–López, Juan Manuel; López-Ramírez, María Patricia; Ramírez-Villanueva, Daniel Alejandro; Dendooven, Luc

doi:10.1099/ijs.0.053629-0

Cited by 15 publications

(3 citation statements)

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“…MIBiG (Minimum Information about a Biosynthetic Gene cluster) alignments yielded one significant result, which was evident by the alignment of a putative S8 peptidase with the SLI-2138 RiPP (ribosomally synthesized and post-translationally modified peptide) biosynthetic gene cluster [ 29 , 30 ]. Similarly, clone 88-1G protein-based phylogeny detected significant similarity with: Candidatus Micrarchaeota archaeon CG1_02_49_24, an uncultured archaeon adapted to high carbon dioxide levels [ 31 ], and the haloalkaliphilic archaea Natronococcus occultus [ 32 ] and Natronobacterium texcoconense [ 33 ]. MIBiG comparison yielded two significant results, which was evident by the alignment of a putative serine protease AprX with the SLI-2138 biosynthetic gene cluster, and the alignment of a putative glycosyltransferase with a glucosyltransferase within a carotenoid biosynthetic gene cluster [ 29 ].…”

Section: Resultsmentioning

confidence: 99%

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Bioprospecting the microbiome of Red Sea Atlantis II brine pool for peptidases and biosynthetic genes with promising antibacterial activity

et al. 2022

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Background The search for novel antimicrobial agents is crucial as antibiotic-resistant pathogens continue to emerge, rendering the available antibiotics no longer effective. Likewise, new anti-cancer drugs are needed to combat the emergence of multi-drug resistant tumors. Marine environments are wealthy sources for natural products. Additionally, extreme marine environments are interesting niches to search for bioactive natural compounds. In the current study, a fosmid library of metagenomic DNA isolated from Atlantis II Deep Lower Convective Layer (ATII LCL), was functionally screened for antibacterial activity as well as anticancer effects. Results Two clones exhibited antibacterial effects against the marine Bacillus Cc6 strain, namely clones 102-5A and 88-1G and they were further tested against eleven other challenging strains, including six safe relatives of ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.), a safe relative to Mycobacterium tuberculosis and four resistant clinical isolates. Clone 88-1G resulted in clear zones of inhibition against eight bacterial strains, while clone 102-5A resulted in zones of inhibition against five bacterial strains. The whole cell lysates of clone 88-1G showed 15% inhibition of Mtb ClpP protease -Mycobacterium tuberculosis drug target-, while whole cell lysates of clone 102-5A showed 19% inhibition of Mtb ClpP protease. Whole cell lysates from the selected clones exhibited anticancer effects against MCF-7 breast cancer cells (cell viability at 50% v/v was 46.2% ± 9.9 for 88-1G clone and 38% ± 7 for 102-5A clone), U2OS osteosarcoma cells (cell viability at 50% v/v was 64.6% ± 12.3 for 88-1G clone and 28.3% ± 1.7 for 102-5A clone) and 1BR hTERT human fibroblast cells (cell viability at 50% v/v was 74.4% ± 5.6 for 88-1G clone and 57.6% ± 8.9 for 102-5A clone). Sequencing of 102-5A and 88-1G clones, and further annotation detected putative proteases and putative biosynthetic genes in clones 102-5A and 88-1G, respectively. Conclusions The ATII LCL metagenome hosts putative peptidases and biosynthetic genes that confer antibiotic and anti-cancer effects. The tested clones exhibited promising antibacterial activities against safe relative strains to ESKAPE pathogens and Mycobacterium tuberculosis. Thus, searching the microbial dark matter of extreme environments is a promising approach to identify new molecules with pharmaceutical potential use.

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Section: Resultsmentioning

confidence: 99%

“…S2). Antimicrobial activities against the ESKAPE pathogens are important because of their emerging antimicrobial resistance, particularly in nosocomial settings [ 32 , 33 ]. Clone 102-5A inhibited the growth of Acinetobacter AB2 strain and MRSA-ZC6.…”

Section: Discussionmentioning

confidence: 99%

Bioprospecting the microbiome of Red Sea Atlantis II brine pool for peptidases and biosynthetic genes with promising antibacterial activity

et al. 2022

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“…Other species are Nbt. texcoconense (isolated from soil, Mexico) [139] and Nbt. nitratireducens (isolated from a soda lake, China) [140].…”

Section: Order Haloferacalesmentioning

confidence: 99%

Systematics of haloarchaea and biotechnological potential of their hydrolytic enzymes

et al. 2017

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Halophilic archaea, also referred to as haloarchaea, dominate hypersaline environments. To survive under such extreme conditions, haloarchaea and their enzymes have evolved to function optimally in environments with high salt concentrations and, sometimes, with extreme pH and temperatures. These features make haloarchaea attractive sources of a wide variety of biotechnological products, such as hydrolytic enzymes, with numerous potential applications in biotechnology. The unique trait of haloarchaeal enzymes, haloenzymes, to sustain activity under hypersaline conditions has extended the range of already-available biocatalysts and industrial processes in which high salt concentrations inhibit the activity of regular enzymes. In addition to their halostable properties, haloenzymes can also withstand other conditions such as extreme pH and temperature. In spite of these benefits, the industrial potential of these natural catalysts remains largely unexplored, with only a few characterized extracellular hydrolases. Because of the applied impact of haloarchaea and their specific ability to live in the presence of high salt concentrations, studies on their systematics have intensified in recent years, identifying many new genera and species. This review summarizes the current status of the haloarchaeal genera and species, and discusses the properties of haloenzymes and their potential industrial applications. SYSTEMATICS OF HALOPHILIC ARCHAEAHalophilic micro-organisms are found in the three domains of life, and are extremophiles living in hypersaline environments in the presence of salt (generally NaCl) as a specific requisite for their growth [1]. Hypersaline environments contain higher salt concentrations than seawater (3.5 % total dissolved salts) [2]. According to the optimum NaCl concentration for growth, micro-organisms fall into one of the following categories: extreme halophiles with optimum growth at 15-30 % (2.5-5.2 M) NaCl; moderate halophiles with optimum growth at 3-15 % (0.5-2.5 M) NaCl; slight halophiles with optimum growth at 1-3 % (0.2-0.5 M) NaCl; non-halophiles with optimum growth at less than 1 % (0.2 M) NaCl; and halotolerant micro-organisms which are non-halophiles showing the ability to tolerate high NaCl concentrations [3]. Halophiles can live in hypersaline environments because they are able to sustain osmotic balance. They use two main strategies to withstand the adverse consequences of water loss when exposed to high salt concentrations. The first strategy used by extremely halophilic archaea (haloarchaea) and halophilic anaerobic bacteria such as members of Halanaerobiales is known as the 'salt-in-cytoplasm' strategy, during which salts such as NaCl or KCl accumulate in the cytoplasm at concentrations similar to the extracellular ones [4,5]. Another mechanism to cope with high salt concentrations is the accumulation of organic molecules such as amino acids, sugars and polyols, known as compatible solutes or osmolytes. Compatible solutes are small and highly soluble molecules that can...

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N atronobacterium

Oren¹

2018

Bergey's Manual of Systematics of Archaea and Bacteria

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Na.tro.no.bac.te'ri.um . N.L. n. natron (arbitrarily derived from the Arabic n. natrun or natron ) soda, sodium carbonate; N.L. pref. natrono‐ , pertaining to soda; N.L. neut. n. bacterium , a small rod; N.L. neut. n. Natronobacterium , soda rod. Euryarchaeota / Halobacteria / Natrialbales / Natrialbaceae / Natronobacterium The genus Natronobacterium (family Natrialbaceae , order Natrialbales , class Halobacteria ) contains two species of haloalkaliphilic, nonmotile, and rod‐shaped or coccoid cells that lyse in distilled water. Colonies are red due to the presence of bacterioruberin carotenoids. The organisms are aerobic chemoorganotrophs; growth is stimulated by some sugars. The major polar lipids are C 20 C 20 and C 20 C 25 glycerol diether derivatives of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester. Minor amounts of glycolipids may be present. The DNA G + C content is between 61.1 and 62.9 mol% (genome sequence). The type strains of the two Natronobacterium species were isolated from a saltern near a hypersaline soda lake in Kenya and from the soil of a dried‐out lake in Mexico. The type species is Natronobacterium gregoryi . DNA G + C content (mol%) : 61.1–62.9 (genome sequence). Type species : Natronobacterium gregoryi Tindall, Ross and Grant 1984b, 355 VP (Effective publication: Tindall, Ross and Grant 1984a, 54).

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Natronobacterium texcoconense sp. nov., a haloalkaliphilic archaeon isolated from soil of a former lake

Cited by 15 publications

References 16 publications

Bioprospecting the microbiome of Red Sea Atlantis II brine pool for peptidases and biosynthetic genes with promising antibacterial activity

Bioprospecting the microbiome of Red Sea Atlantis II brine pool for peptidases and biosynthetic genes with promising antibacterial activity

Systematics of haloarchaea and biotechnological potential of their hydrolytic enzymes

N atronobacterium

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