Natterins, a new class of proteins with kininogenase activity characterized from fish venom

Magalhães, Geraldo Santana; Lopes-Ferreira, Mônica; Junqueira-de-Azevedo, Inácio L.M.; Spencer, Patrick Jack; Araújo, Magali; Portaro, Fernanda Calheta Vieira; Ma, Liyuan; Valente, Richard H.; Juliano, Luiz; Fox, Jay W.

doi:10.1016/j.biochi.2005.03.016

Cited by 105 publications

(70 citation statements)

References 27 publications

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“…The qPCR primers used above matched conserved gene regions and thus estimate the combined expression of several paralogs. We chose to measure the expression of three different natterin paralogs ( nattl1, 2 and 3 ), in part because this understudied gene was first characterized as a toxin produced by a tropical fish 75, 76 . We studied nattl expression in several developmental stages in AC-, SB- and PL-charr as well as in selected tissues of adult AC-charr.…”

Section: Resultsmentioning

confidence: 99%

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The developmental transcriptome of contrasting Arctic charr (Salvelinus alpinus) morphs

et al. 2016

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Species and populations with parallel evolution of specific traits can help illuminate how predictable adaptations and divergence are at the molecular and developmental level. Following the last glacial period, dwarfism and specialized bottom feeding morphology evolved rapidly in several landlocked Arctic charr Salvelinus alpinus populations in Iceland. To study the genetic divergence between small benthic morphs and limnetic morphs, we conducted RNA-sequencing charr embryos at four stages in early development. We studied two stocks with contrasting morphologies: the small benthic (SB) charr from Lake Thingvallavatn and Holar aquaculture (AC) charr.The data reveal significant differences in expression of several biological pathways during charr development. There was also an expression difference between SB- and AC-charr in genes involved in energy metabolism and blood coagulation genes. We confirmed differing expression of five genes in whole embryos with qPCR, including lysozyme and natterin-like which was previously identified as a fish-toxin of a lectin family that may be a putative immunopeptide. We also verified differential expression of 7 genes in the developing head that associated consistently with benthic v.s.limnetic morphology (studied in 4 morphs). Comparison of single nucleotide polymorphism (SNP) frequencies reveals extensive genetic differentiation between the SB and AC-charr (~1300 with more than 50% frequency difference). Curiously, three derived alleles in the otherwise conserved 12s and 16s mitochondrial ribosomal RNA genes are found in benthic charr.The data implicate multiple genes and molecular pathways in divergence of small benthic charr and/or the response of aquaculture charr to domestication. Functional, genetic and population genetic studies on more freshwater and anadromous populations are needed to confirm the specific loci and mutations relating to specific ecological traits in Arctic charr.

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Section: Resultsmentioning

confidence: 99%

“…Another gene that caught our attention was natterin-like . Natterins were first discovered from the venom gland of the tropical toxic fish species Thalassophryne nattereri 75, 76 , and are found by sequence similarity in e.g. zebrafish, Atlantic salmon and here in Arctic charr.…”

Section: Discussionmentioning

confidence: 99%

The developmental transcriptome of contrasting Arctic charr (Salvelinus alpinus) morphs

et al. 2016

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“…The qPCR analyses confirmed reciprocal regulation between defensin defb1-like (down-regulated) and cathelicidin (cath) and natterin-like (nattl), which showed highest activation at 3 dpc with both hormones. The first two are antimicrobial proteins while nattl might encode a toxin with similar properties as natterin that was first identified in the venom of fish Thalassophryne nattereri (Magalhães et al, 2005). Changes of Th cells were also addressed by qPCR.…”

Section: Real-time Qpcrmentioning

confidence: 99%

Sexual maturation and administration of 17β-estradiol and testosterone induce complex gene expression changes in skin and increase resistance of Atlantic salmon to ectoparasite salmon louse

Krasnov

Breiland

Hatlen

et al. 2015

General and Comparative Endocrinology

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“…Furthermore, internal peptides of natterins 1 to 3 were found in major spots of whole venom submitted to mass spectrometry/2DGE. Similarly to ESTs, the complete sequences of natterins did not show any significant similarity with already described tissue kallikreins, kininogenases or any proteinase, all being entirely new [12,13]. …”

Section: Reviewmentioning

confidence: 99%

Thalassophryne nattereri fish venom: from the envenoming to the understanding of the immune system

Lopes-Ferreira

Grund

Lima

2014

J Venom Anim Toxins incl Trop Dis

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Thalassophryne nattereri (niquim) is a venomous fish found off North and Northeast coast of Brazil, where it is known by the severity of the accidents involving humans. This review article is divided into four topics. The first one provides a brief description of the animal biology and its distribution off Brazilian coastal waters, the venom apparatus, signs and symptoms observed in envenomated humans and also describes envenomation in mice. The second topic describes the use of modern genetic approach and mass spectrometry for identification of highly expressed genes in its venom glands and the sequence of major toxins. The third chapter offers a detailed study of tissue injury induced by the venom and reveals the role of toxins that impair inflammation reduction. Finally, the fourth section expands the understanding of many extrinsic and intrinsic essential factors in maintaining survival of memory B cell compartment. Our results demonstrate the wide possibilities for research in the area of toxinology, also the necessity of interconnection among biochemistry, pharmacology and immunology areas for the expansion of knowledge and for generation of innovation.

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Natterins, a new class of proteins with kininogenase activity characterized from fish venom

Cited by 105 publications

References 27 publications

The developmental transcriptome of contrasting Arctic charr (Salvelinus alpinus) morphs

The developmental transcriptome of contrasting Arctic charr (Salvelinus alpinus) morphs

Sexual maturation and administration of 17β-estradiol and testosterone induce complex gene expression changes in skin and increase resistance of Atlantic salmon to ectoparasite salmon louse

Thalassophryne nattereri fish venom: from the envenoming to the understanding of the immune system

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