Nattokinase crude extract enhances oral mucositis healing

Abstract

Background Nattokinase (NK) is a promising alternative in the prevention and treatment of cardiovascular diseases due to its potent fibrinolytic activity. In this study, we investigated the effect of crude nattokinase extract on the healing of acetic acid-induced oral mucositis in mice. Methods Bacillus subtilis culture media (BSCM) was isolated into the supernatant, named nattokinase crude extract (NCE), and the pellet was named Bacillus subtilis mass (BSM). An oral mucositis model was established in mice by applying 50% glacial acetic acid to the buccal mucosa. According to the treatment conditions, the mice were divided into BSCM, NCE, BSM and phosphate buffered saline (PBS) groups. The weight of the mice, oral mucositis healing score and histopathological examination were used to evaluate the treatment. Results Fibrinolytic activities of BSCM, NCE and BSM were approximately 8069, 10,800 and 80 U/ml, respectively. The weight gain of mice in the NCE group was significantly different from the PBS group after three days’ treatment (p < 0.05). The oral mucositis score of NCE group was significantly higher than other groups (p < 0.05). The differences in histopathology scores between the NCE and other groups were statistically significant (p < 0.01). Conclusions NCE could possess remarkable potential to reduce pain and promote oral mucositis healing with minimal safety concerns. In this study, we first report that NCE from the supernatant of Bacillus subtilis can promote the healing of oral mucositis, which extends the application scope of NK.

A local dark tea – Liubao tea – extract exhibits remarkable performance in oral tissue regeneration, inflammation relief and oral microbiota reconstruction

“…This phenomenon may be attributed to the development of oral mucositis and inflammation induced by the application of acetic acid, resulting in pain and discomfort while eating and swallowing. 43,44 Then, on days 3 and 4, the food intake of mice in groups L and H gradually recovered following LTE application, while group M only demonstrated a slight increase (Fig. 2c).…”

Microbial nattokinase: from synthesis to potential application

“…134 It has also been found that NK inhibits the restenosis of rabbit abdominal arteries after percutaneous transluminal angioplasty and exhibits significant potential to reduce pain and promote healing of oral mucositis. 135,136 Furthermore, ascites and tumor growth were significantly reduced and the survival rate increased to 31% after feeding NK crude extract to mice with liver cancer (Fig. 4).…”

Biotechnology, Bioengineering and Applications of Bacillus Nattokinase

“…In addition to the research of nattokinase in the fields of thrombosis and hypertension treatment, nattokinase can also be used as a drug or adjuvant to treat diseases such as cancer [ 11 ], Alzheimer’s disease (AD) [ 104 ], retinopathy [ 10 ], hyperlipidemia [ 105 ] and oral mucositis [ 106 ]. Nattokinase has broad prospects in the field of cancer treatment and is expected to become an effective means of cancer treatment in the future.…”