NATURAL ANTHRAQUINONEFROM THE BARK OF Cinchona officinalis L.

Abstract

Cinchona officinalis is known as the source of alkaloid compounds. Almost 7-12 % of alkaloids were contained in its bark. Not many studies reported other chemical compounds in it. This research aimed to isolate non-alkaloid compounds in the C. officinalis bark. It was extracted by maceration using n-hexane, ethyl acetate, and ethanol. The alkaloidal fraction was separated from ethylacetate extract based on acid-base reaction. The fractions were continued to the next step of fractionations using vacuum liquid chromatography (VLC) and radial chromatography to yield compound 1. Characterizations of compound 1 were done by spectroscopic methods (UV, IR, ESI-MS, NMR ( 1 H-, 13 C-, HSQC, and HMBC)).The maximum wavelengths of compound 1 were 278 and 430 nm. 1 H-dan 13 C-NMR data showed that compound 1 consisted of ten hydrogens and fifteen carbons. ESI-MS showed that the molecular weight was 285.96. It was predicted as 1,3,8-trihydroxy-2-methoxy-9,10-anthraquinone. It was firstly isolated from the ethyl acetate extract of C.officinalis bark.

TOXICITY AND ANTIOXIDANT ACTIVITIES OF EXTRACT OF n-HEXANE, H2O, AND ETHYL ACETATE FROM THE LEAVES OF DURIAN, Durio zibethinus L.

“…The leaves of durian (D. zibethinus L.) powder (1.8 kg) macerated with ethanol 96% 6 times. Maceration process that had done at room temperature 4 . Then partitioned with n-hexane and ethyl acetate 3 times.…”

ISOLATION AND DETERMINATION OF CHEMICAL COMPOUNDS FROM N-HEXANE FRACTION OF THE SINTOK STEM BARK (Cinnamomum sintoc Bl.)

“…Raji et.al, for example, reported secondary metabolites like alkaloids, flavonoids, tannins, and saponins were extracted from four medicinal plants viz. Cassia alata, Thespesia populnea, Euphorbia hirta, and Wrightia tinctoria and characterized by UV, TLC, FTIR and GCMS analysis39 . Insanu et.al mentioned isolation non-alkaloid compounds in the Cinchona.…”