Natural anti-galactose α1,3 galactose antibodies delay, but do not prevent the acceptance of extracellular matrix xenografts

Raeder, Roberta; Badylak, Stephen F.; Sheehan, Christine; Kallakury, Bhaskar; Metzger, Dennis W.

doi:10.1016/s0966-3274(01)00044-2

Cited by 85 publications

(49 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[17][18][19] Several studies have shown that SIS can provide a collagenous matrix to promote cell migration into the healing site to enhance revascularization and repair. [20][21][22][23][24] It is hypothesized that SIS enhance and promote the healing of the ACL. Instead of using the SIS as a load-bearing 10-layered bioscaffold, like in the rotator cuff repair, only a single layer of SIS was used to recruit sufficient healing cells to the hypocellular ACL.…”

Section: Introductionmentioning

confidence: 99%

Healing of the Goat Anterior Cruciate Ligament After a New Suture Repair Technique and Bioscaffold Treatment

Nguyen

Geel

Schulze

et al. 2013

Tissue Engineering Part A

View full text Add to dashboard Cite

Primary suture repair of the anterior cruciate ligament (ACL) has been used clinically in an attempt to heal the ruptured ACL. The results, however, were not satisfactory, which in retrospect can be attributed to the used suturing technique and the suboptimal healing conditions. These constraining conditions can be improved by introducing a new suturing technique and by using small intestinal submucosa (SIS) as a bioscaffold. It is hypothesized that the suturing technique keep the torn ends together and that SIS enhance and promote the healing of the ACL. The goat was used as the study model. In the Suture group, the left ACL was transected and suture repaired with a new locking suture repair technique (n = 5) allowing approximation and fixation under tension. The Suture-SIS group underwent the same procedure with the addition of SIS (n = 5). The right ACL served as control. After 12 weeks of healing, anterior-posterior translation and in situ force of the healing ACL were measured, followed by the measurement of the cross-sectional area and structural stiffness. Routine histology was performed on tissue samples. Gross morphology showed that the healing ACL was continuous with collagenous tissue in both groups. The cross-sectional area of the Suture and the Suture-SIS group was 35% and 50% of the intact control, respectively. The anterior-posterior translations at different flexion angles were statistically not different between the Suture group and the Suture-SIS group. Only the in situ force at 30°in the Suture-SIS group was higher than in the Suture group. Tensile tests showed that the stiffness for the Suture group was not different from the Suture-SIS group (31.1 -8.1 N/mm vs. 41.9 -18.0 N/mm [p > 0.05]). Histology showed longitudinally aligned collagen fibers from origo to insertion. More fibroblasts were present in the healing tissue than in the control intact tissue. The study demonstrated the proof of concept of ACL repair in a goat model with a new suture technique and SIS. The mechanical outcome is not worse than previously reported for ACL reconstruction. In conclusion, the approach of using a new suture technique, with or without a bioscaffold to heal the ACL is promising.

show abstract

Section: Introductionmentioning

confidence: 99%

Healing of the Goat Anterior Cruciate Ligament After a New Suture Repair Technique and Bioscaffold Treatment

Nguyen

Geel

Schulze

et al. 2013

Tissue Engineering Part A

View full text Add to dashboard Cite

show abstract

“…Although studies have shown that the aGal epitopes in ECM bioscaffolds did not affect tissue remodeling over the long term in animal models, serologic changes and a delay of graft acceptance have been observed [37,38]. In cases where a large amount of the bioscaffold is used for tissue repair or augmentation, removal of aGal in these bioscaffolds may provide certain benefits [1,21,22,39].…”

Section: Introductionmentioning

confidence: 99%

Alpha1,3-galactosyltransferase knockout does not alter the properties of porcine extracellular matrix bioscaffolds

et al. 2011

View full text Add to dashboard Cite

“…28 However, when present in sufficiently small quantities, neither DNA nor the a-gal epitope has been associated with poor outcomes in vivo. 29,30 The objective of the present study was to characterize the adipose ECM material resulting from three distinct methods of decellularization-one method developed and optimized by the authors and two previously published methods-to determine the most effective method for the derivation of an adipose tissue ECM scaffold that was largely free of potentially immunogenic cellular content, including DNA and cytoplasmic lipid, while retaining adipose tissue-specific structural and functional components. The efficacy of removal of cellular content, the effect upon ultrastructure, maintenance of several ECM components and growth factors, and the ability of the resulting material to support the in vitro growth and differentiation of adipose-derived stem cells (ADSCs) toward an adipogenic lineage were investigated for each method.…”

mentioning

confidence: 99%

Comparison of Three Methods for the Derivation of a Biologic Scaffold Composed of Adipose Tissue Extracellular Matrix

Brown

Freund

Han

et al. 2011

Tissue Engineering Part C: Methods

195

197

View full text Add to dashboard Cite

Natural anti-galactose α1,3 galactose antibodies delay, but do not prevent the acceptance of extracellular matrix xenografts

Cited by 85 publications

References 23 publications

Healing of the Goat Anterior Cruciate Ligament After a New Suture Repair Technique and Bioscaffold Treatment

Healing of the Goat Anterior Cruciate Ligament After a New Suture Repair Technique and Bioscaffold Treatment

Alpha1,3-galactosyltransferase knockout does not alter the properties of porcine extracellular matrix bioscaffolds

Comparison of Three Methods for the Derivation of a Biologic Scaffold Composed of Adipose Tissue Extracellular Matrix

Contact Info

Product

Resources

About