Natural antisense RNA inhibits the expression of BCMA, a tumour necrosis factor receptor homologue.

Hatzoglou, Anastassia; Deshayes, Frédérique; Madry, Christine; Laprée, Geneviève; Castanas, Elias; Tsapis, Andréas

doi:10.1186/1471-2199-3-4

Cited by 27 publications

(9 citation statements)

References 30 publications

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“…Antisense BCMA RNA is transcribed from the same locus as BCMA and has typical mRNA features, e.g., polyadenylation, splicing, Kozak consensus sequence and an open reading frame encoding an experimentally proven 115 amino acid peptide: p12 protein [107]. Experimental data suggests that antisense BCMA inhibits the expression of BCMA protein, while it does not affect the expression level of BCMA mRNA.…”

Section: Regulatory Functions Of Lncrnas and Mrnasmentioning

confidence: 99%

“…Experimental data suggests that antisense BCMA inhibits the expression of BCMA protein, while it does not affect the expression level of BCMA mRNA. The inhibition of BCMA expression is obtained through the action of the antisense RNA and not of the p12 protein, although the exact mechanism is not fully understood [107]. …”

Section: Regulatory Functions Of Lncrnas and Mrnasmentioning

confidence: 99%

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Regulatory Roles for Long ncRNA and mRNA

Karapetyan

Buiting

Kuiper

et al. 2013

Cancers

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Recent advances in high-throughput sequencing technology have identified the transcription of a much larger portion of the genome than previously anticipated. Especially in the context of cancer it has become clear that aberrant transcription of both protein-coding and long non-coding RNAs (lncRNAs) are frequent events. The current dogma of RNA function describes mRNA to be responsible for the synthesis of proteins, whereas non-coding RNA can have regulatory or epigenetic functions. However, this distinction between protein coding and regulatory ability of transcripts may not be that strict. Here, we review the increasing body of evidence for the existence of multifunctional RNAs that have both protein-coding and trans-regulatory roles. Moreover, we demonstrate that coding transcripts bind to components of the Polycomb Repressor Complex 2 (PRC2) with similar affinities as non-coding transcripts, revealing potential epigenetic regulation by mRNAs. We hypothesize that studies on the regulatory ability of disease-associated mRNAs will form an important new field of research.

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Section: Regulatory Functions Of Lncrnas and Mrnasmentioning

confidence: 99%

Section: Regulatory Functions Of Lncrnas and Mrnasmentioning

confidence: 99%

Regulatory Roles for Long ncRNA and mRNA

Karapetyan

Buiting

Kuiper

et al. 2013

Cancers

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“…Some studies had suggested that cis -NATs represent transcriptional noise [11,12], while others had reported supportive evidence for the function of various cis -NATs [13-16], especially in RNA editing [17], stability [18], and translation [19]. Growing evidence implicating a role of NATs in medical conditions, such as hypertension [20] and immune disorders [21], suggests a functional role for cis -NATs. However, it cannot be assumed that cis -NATs are as actively regulated as its sense counterparts.…”

Section: Introductionmentioning

confidence: 99%

Conserved expression of natural antisense transcripts in mammals

et al. 2013

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BackgroundRecent studies had found thousands of natural antisense transcripts originating from the same genomic loci of protein coding genes but from the opposite strand. It is unclear whether the majority of antisense transcripts are functional or merely transcriptional noise.ResultsUsing the Affymetrix Exon array with a modified cDNA synthesis protocol that enables genome-wide detection of antisense transcription, we conducted large-scale expression analysis of antisense transcripts in nine corresponding tissues from human, mouse and rat. We detected thousands of antisense transcripts, some of which show tissue-specific expression that could be subjected to further study for their potential function in the corresponding tissues/organs. The expression patterns of many antisense transcripts are conserved across species, suggesting selective pressure on these transcripts. When compared to protein-coding genes, antisense transcripts show a lesser degree of expression conservation. We also found a positive correlation between the sense and antisense expression across tissues.ConclusionOur results suggest that natural antisense transcripts are subjected to selective pressure but to a lesser degree compared to sense transcripts in mammals.

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“…For example, duplex formation between sense and antisense RNAs in the nucleus can modulate mRNA alternative splicing, editing and transport345. Sense-antisense duplex formation in the cytoplasm can change sense mRNA stability and translation efficiency678910. It has also been suggested that some antisense transcripts bind to the corresponding DNA strand and recruit DNA methyltransferases or histone-modifying enzymes, thereby modulating sense gene expression111213.…”

mentioning

confidence: 99%

ASBEL, an ANA/BTG3 antisense transcript required for tumorigenicity of ovarian carcinoma

Yanagida

Taniue

Sugimasa

et al. 2013

Sci Rep

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Mammalian genomes encode numerous antisense non-coding RNAs, which are assumed to be involved in the regulation of the sense gene expression. However, the mechanisms of their action and involvement in the development of diseases have not been well elucidated. The ANA/BTG3 protein is an antiproliferative protein whose expression is downregulated in prostate and lung cancers. Here we show that an antisense transcript of the ANA/BTG3 gene, termed ASBEL, negatively regulates the levels of ANA/BTG3 protein, but not of ANA/BTG3 mRNA and is required for proliferation and tumorigenicity of ovarian clear cell carcinoma. We further show that knockdown of ANA/BTG3 rescues growth inhibition caused by ASBEL knockdown. Moreover, we demonstrate that ASBEL forms duplexes with ANA/BTG3 mRNA in the nucleus and suppresses its cytoplasmic transportation. Our findings illustrate a novel function for an antisense transcript that critically promotes tumorigenesis by suppressing translation of the sense gene by inhibiting its cytoplasmic transportation.

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Natural antisense RNA inhibits the expression of BCMA, a tumour necrosis factor receptor homologue.

Cited by 27 publications

References 30 publications

Regulatory Roles for Long ncRNA and mRNA

Regulatory Roles for Long ncRNA and mRNA

Conserved expression of natural antisense transcripts in mammals

ASBEL, an ANA/BTG3 antisense transcript required for tumorigenicity of ovarian carcinoma

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