Natural association of Gluconacetobacter diazotrophicus and diazotrophic Acetobacter peroxydans with wetland rice

Muthukumarasamy, Ramachandran; Cleenwerck, Ilse; Revathi, G.; Vadivelu, M.; Janssens, Daniëlle; Hoste, Bart; Gum, Kang Ui; Park, Ki-Do; Son, Cho Young; Sa, Tongmin; Caballero‐Mellado, Jesús

doi:10.1016/j.syapm.2005.01.006

Cited by 97 publications

(53 citation statements)

References 35 publications

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“…For 135 of these strains, DNA-DNA hybridization data or 16S-23S rRNA gene spacer region sequence analysis data have been reported previously (Urakami et al, 1989;Sievers et al, 1992;Boesch et al, 1998;Sokollek et al, 1998;Franke et al, 1999;Navarro et al, 1999;Lisdiyanti et al, 2000Lisdiyanti et al, , 2001Lisdiyanti et al, , 2002Lisdiyanti et al, , 2006Schüller et al, 2000;Yamada et al, 2000;Fuentes-Ramírez et al, 2001;Katsura et al, 2001;Cleenwerck et al, 2002Cleenwerck et al, , 2007Cleenwerck et al, , 2008Trček & Teuber, 2002;Loganathan & Nair, 2004;Jojima et al, 2004;Yukphan et al, 2004Yukphan et al, , 2005Dellaglio et al, 2005;Muthukumarasamy et al, 2005;Sievers & Swings, 2005;Dutta & Gachhui, 2006, 2007Greenberg et al, 2006;Silva et al, 2006;Takahashi et al, 2006;Camu et al, 2007;Malimas et al, 2007;Ndoye et al, 2007;. Therefore, these strains were assumed to be accurately classified at the species level and were regarded as reference strains for the purpose of this study.…”

mentioning

confidence: 69%

Differentiation of species of the family Acetobacteraceae by AFLP DNA fingerprinting: Gluconacetobacter kombuchae is a later heterotypic synonym of Gluconacetobacter hansenii

Cleenwerck

Wachter

González

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Amplified fragment length polymorphism (AFLP) DNA fingerprinting was investigated as a tool for fast and accurate identification of acetic acid bacteria (AAB) to the species level. One hundred and thirty five reference strains and 15 additional strains, representing 50 recognized species of the family Acetobacteraceae, were subjected to AFLP analysis using the restriction enzyme combination ApaI/ TaqI and the primer combination A03/T03. The reference strains had been previously subjected to either DNA-DNA hybridization or 16S-23S rRNA spacer region gene sequence analysis and were regarded as being accurately classified at the species level. The present study revealed that six of these strains should be reclassified, namely Gluconacetobacter europaeus LMG 1518 and Gluconacetobacter xylinus LMG 1510 as Gluconacetobacter xylinus and Gluconacetobacter europaeus, respectively; Gluconacetobacter kombuchae LMG 23726 T as Gluconacetobacter hansenii; and Acetobacter orleanensis strains LMG 1545, LMG 1592 and LMG 1608 as Acetobacter cerevisiae. Cluster analysis of the AFLP DNA fingerprints of the reference strains revealed one cluster for each species, showing a linkage level below 50 % with other clusters, except for Acetobacter pasteurianus, Acetobacter indonesiensis and Acetobacter cerevisiae. These three species were separated into two, two, and three clusters, respectively. At present, confusion exists regarding the taxonomic status of Gluconacetobacter oboediens and Gluconacetobacter intermedius; the AFLP data from this study supported their classification as separate taxa. The 15 additional strains could all be identified at the species level. AFLP analysis further revealed that some species harboured genetically diverse strains, whereas other species consisted of strains showing similar banding patterns, indicating a more limited genetic diversity. It can be concluded that AFLP DNA fingerprinting is suitable for accurate identification and classification of a broad range of AAB, as well as for the determination of intraspecific genetic diversity.Acetic acid bacteria (AAB) are Gram-negative, coccoid or rod-shaped, obligate aerobic bacteria that have the ability to incompletely oxidize a wide range of carbohydrates, alcohols and sugar alcohols. They are involved in the production of several fermented foods and beverages, either in a beneficial (vinegars, cocoa-based products, Kombucha and nata de coco) or detrimental (spoilage of beer, wine and cider) manner and are also used in the production of commercially important fine chemicals and bacterial cellulose. Recently, some AAB have beenAbbreviations: AAB, acetic acid bacteria; AFLP, amplified fragment length polymorphism; ERIC enterobacterial repetitive intergenic consensus sequences; FAFLP, fluorescent amplified fragment length polymorphism; RAPD, randomly amplified polymorphic DNA; REP, Repetitive extragenic palindromic sequences.The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of LMG 23726 T is AM999342.A supplementary figure showing the (GTG...

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mentioning

confidence: 69%

Differentiation of species of the family Acetobacteraceae by AFLP DNA fingerprinting: Gluconacetobacter kombuchae is a later heterotypic synonym of Gluconacetobacter hansenii

Cleenwerck

Wachter

González

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…Moreover, insects from which AAB are usually recovered have sugar-rich diets that are unbalanced for nitrogen content, so they have to fulfill the nitrogen requirement from other sources, such as the atmosphere (55). Among AAB, several examples of N 2 -fixing bacteria have been reported, such as Ga. diazotrophicus (32,66), Ga. johannae, Ga. azotocaptans (30), Ga. kombuchae (21), A. peroxydans (54), A. nitrogenifigens (20), and Swaminathania salitolerans (45). However, further investigations are needed to evaluate if insect-associated AAB could contribute to insect nitrogen metabolism or recycling.…”

Section: The Insect Body Can Be An Appropriate Niche For Aabmentioning

confidence: 99%

Acetic Acid Bacteria, Newly Emerging Symbionts of Insects

Crotti

Rizzi

Chouaia

et al. 2010

Appl Environ Microbiol

296

260

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8Recent research in microbe-insect symbiosis has shown that acetic acid bacteria (AAB) establish symbiotic relationships with several insects of the orders Diptera, Hymenoptera, Hemiptera, and Homoptera, all relying on sugar-based diets, such as nectars, fruit sugars, or phloem sap. To date, the fruit flies Drosophila melanogaster and Bactrocera oleae, mosquitoes of the genera Anopheles and Aedes, the honey bee Apis mellifera, the leafhopper Scaphoideus titanus, and the mealybug Saccharicoccus sacchari have been found to be associated with the bacterial genera Acetobacter, Gluconacetobacter, Gluconobacter, Asaia, and Saccharibacter and the novel genus Commensalibacter. AAB establish symbiotic associations with the insect midgut, a niche characterized by the availability of diet-derived carbohydrates and oxygen and by an acidic pH, selective factors that support AAB growth. AAB have been shown to actively colonize different insect tissues and organs, such as the epithelia of male and female reproductive organs, the Malpighian tubules, and the salivary glands. This complex topology of the symbiosis indicates that AAB possess the keys for passing through body barriers, allowing them to migrate to different organs of the host. Recently, AAB involvement in the regulation of innate immune system homeostasis of Drosophila has been shown, indicating a functional role in host survival. All of these lines of evidence indicate that AAB can play different roles in insect biology, not being restricted to the feeding habit of the host. The close association of AAB and their insect hosts has been confirmed by the demonstration of multiple modes of transmission between individuals and to their progeny that include vertical and horizontal transmission routes, comprising a venereal one. Taken together, the data indicate that AAB represent novel secondary symbionts of insects.

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“…In agriculture, crop productivity is mainly governed by the use of inorganic N-fertilizers, which are expensive and damaging to the environment 32) . Finding alternative ways of supplying nitrogen to grasses and cereals has been a major research challenge in tropical agriculture 1,3) .…”

mentioning

confidence: 99%

Enhancement of Nitrogen-fixing Activity of Enterobacteriaceae Strains Isolated from Sago Palm (Metroxylon sagu) by Microbial Interaction with Non-nitrogen Fixers

et al. 2007

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This study was done to determine microbial interactions between nitrogen-fixing bacteria (NFB) and different indigenous bacteria, both of which were isolated from the same samples of sago palm. Co-culture of NFB and indigenous bacteria, isolated using nutrient agar medium, into a nitrogen-free Rennie medium showed significantly higher nitrogen-fixing activity (NFA) than single inoculations of NFB did in almost all combinations. A reduced oxygen status also enhanced the NFA of NFB. All NFB preferred simple sugars as their substrates for NFA and showed extremely low levels of NFA in starch, hemicellulose, and pectin-containing media. NFA was markedly stimulated in the consortium of starch-degrading Bacillus sp. strain B1 and NFB, ranging from 0-1.5 to 150-270 nmol C2H4 culture −1 h −1 . The consortium of hemicellulose-degrading Agrobacterium sp. strain HMC1 or Flexibacter sp. strain HMC2 and NFB also showed enhanced NFA, ranging from 0-0.1 to 16-38 nmol. In contrast, no enhanced NFA was detected in the consortium of pectin-degrading Burkholderia sp. strain BT1 or Paenibacillus sp. strain P1 and NFB. These results may indicate that beneficial microbial interactions occur in sago palm to enhance nitrogen-fixing activity through collaborative utilizations of starch, hemicellulose and their degradation products.

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Natural association of Gluconacetobacter diazotrophicus and diazotrophic Acetobacter peroxydans with wetland rice

Cited by 97 publications

References 35 publications

Differentiation of species of the family Acetobacteraceae by AFLP DNA fingerprinting: Gluconacetobacter kombuchae is a later heterotypic synonym of Gluconacetobacter hansenii

Differentiation of species of the family Acetobacteraceae by AFLP DNA fingerprinting: Gluconacetobacter kombuchae is a later heterotypic synonym of Gluconacetobacter hansenii

Acetic Acid Bacteria, Newly Emerging Symbionts of Insects

Enhancement of Nitrogen-fixing Activity of Enterobacteriaceae Strains Isolated from Sago Palm (Metroxylon sagu) by Microbial Interaction with Non-nitrogen Fixers

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