Natural Astaxanthin Improves Testosterone Synthesis and Sperm Mitochondrial Function in Aging Roosters

Gao, Shan; Zhao, Bang-Xin; Long, Cheng; Heng, Nuo; Guo, Yuanqiang; Sheng, Xihui; Wang, Xiangguo; Xing, Kai; Xiao, Longfei; Ni, Hemin; Wu, Shu‐geng; Qi, Xiaolong

doi:10.3390/antiox11091684

Cited by 8 publications

(2 citation statements)

References 63 publications

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“…Scavenging activities of hydroxyl free radicals were measured as described by previous study [ 53 ]. A commercial kit (Nanjing Jiancheng Bioengineering Institute; Product code A018-1-1, Colorimetric method) was used according to the manufacturer’s protocol.…”

Section: Methodsmentioning

confidence: 99%

Identification of Antioxidative Peptides Derived from Arthrospira maxima in the Biorefinery Process after Extraction of C-Phycocyanin and Lipids

et al. 2023

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Arthrospira maxima has been identified as a sustainable source of rich proteins with diverse functionalities and bioactivities. After extracting C-phycocyanin (C-PC) and lipids in a biorefinery process, the spent biomass still contains a large proportion of proteins with potential for biopeptide production. In this study, the residue was digested using Papain, Alcalase, Trypsin, Protamex 1.6, and Alcalase 2.4 L at different time intervals. The resulting hydrolyzed product with the highest antioxidative activity, evaluated through their scavenging capability of hydroxyl radicals, superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), was selected for further fractionation and purification to isolate and identify biopeptides. Alcalase 2.4 L was found to produce the highest antioxidative hydrolysate product after four-hour hydrolysis. Fractionating this bioactive product using ultrafiltration obtained two fractions with different molecular weights (MW) and antioxidative activity. The low-molecular-weight fraction (LMWF) with MW <3 kDa had higher DPPH scavenging activity with the IC50 value of 2.97 ± 0.33 compared to 3.76 ± 0.15 mg/mL of the high-molecular-weight fraction (HMWF) with MW >3 kDa. Two stronger antioxidative fractions (F-A and F-B) with the respective significant lower IC50 values of 0.83 ± 0.22 and 1.52 ± 0.29 mg/mL were isolated from the LMWF using gel filtration with a Sephadex G-25 column. Based on LC-MS/MS analysis of the F-A, 230 peptides derived from 108 A. maxima proteins were determined. Notably, different antioxidative peptides possessing various bioactivities, including antioxidation, were detected with high predicted scores together with in silico analyses on their stability and toxicity. This study established knowledge and technology to further value-add to the spent A. maxima biomass by optimizing hydrolysis and fraction processes to produce antioxidative peptides with Alcalase 2.4 L after two products already produced in a biorefinery. These bioactive peptides have potential applications in food and nutraceutical products.

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Section: Methodsmentioning

confidence: 99%

Identification of Antioxidative Peptides Derived from Arthrospira maxima in the Biorefinery Process after Extraction of C-Phycocyanin and Lipids

et al. 2023

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“…The testes are the primary reproductive organs in roosters responsible for sperm production and testosterone synthesis [13,14]. There is ample evidence that the reduction in the activity of the gonadal axis associated with declining reproductive behavior is related to the aging phenomenon [15]. During the aging process, the morphological changes that occur in the testes are mainly characterized by a decrease in the volume and number of germ cells, as well as a decrease in semen volume and quality [5].…”

Section: Introductionmentioning

confidence: 99%

Dietary Restriction Promote Sperm Remodeling in Aged Roosters Based on Transcriptome Analysis

liang,

he,

zhu

et al. 2024

Preprint

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Background: The breeder rooster has played an important role in poultry production by providing high-quality semen. In general, the fertility peaks of breeder roosters between 30 and 40 weeks of age and declines rapidly from 45 to 55 weeks of age. The exploration of improving fertility in aging roosters would help to extend the useful life of roosters. At present, researchers have made some progress, but improving fertility in aging roosters has become an important issue that needs to be addressed. Methods: To identify the genes related to promoting sperm remodeling in aged Houdan roosters, we combined changes in testis and semen quality with transcriptome sequencing (RNA-seq) to analyze the synchrony of semen quality and testis development. In this study, 350-day-old Houdan breeder roosters were selected for RNA-seq analysis in testis tissues from induced molting roosters (D group) and non-induced molting roosters (47DG group). All analyses of differentially expressed genes (DEGs) and functional enrichment were performed. Finally, we selected six DEGs to verify the accuracy of the sequencing by qPCR. Results: Sperm motility, sperm density, and testis weight were significantly increased in roosters in the fasted-induced molting group compared with the control group (P < 0.05). Further RNA-seq analysis of the testis between the D group and 47DG group identified 61 DEGs, with 21 up-regulated and 40 down-regulated. Functional enrichment analysis showed that the DEGs were primarily enriched in the cytokine-cytokine receptor interaction, Wnt signaling pathway, MAPK signaling pathway, TGF-β signaling pathway, and focal adhesion pathway. The qRT-PCR results showed that the expression trend of these genes was consistent with the sequencing results. WNT5A, FGFR3, AGTR2, TGFβ2, ROMO1, and SLC26A7 may play a role in testis development and spermatogenesis. This study provides fundamental data to enhance the reproductive value of aging roosters.

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Alpha‐Linolenic Acid Supplementation Improves Testosterone Production in an Aged Breeder Rooster Model: Role of Mitochondrial Modulation and SIRT1 Activation

Long,

Zhao,

Willing

et al. 2024

Molecular Nutrition Food Res

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ScopeAging in males can lead to declines in testosterone production, essential for maintaining male reproductive health.Methods and resultsTo investigate the effects of dietary supplementation with alpha‐linolenic acid (ALA) on testosterone production in aged breeder roosters and understand the underlying molecular mechanisms involved. An in vivo model is established to investigate the effects of dietary ALA supplementation on testosterone production in aged breeder roosters, and the Leydig cell culture is used to identify the potential molecular mechanism. Dietary supplementation with ALA increases in plasma testosterone. Congruently, ALA supplementation enhances the expression of testosterone biosynthesis‐related enzymes. ALA supplementation exerts anti‐apoptotic effects in testicular mitochondria, as evidenced by a lower expression of pro‐apoptotic factors and a higher expression of the anti‐apoptotic factor B‐cell lymphoma 2 (Bcl‐2). Moreover, In Leydig cells, ALA supplementation promotes mitochondrial biogenesis genes. The proposed mechanism is that ALA activates the sirtuin1 (SIRT1) pathway and is supported by higher SIRT1 transcript and protein in Leydig cells. Furthermore, blocking SIRT1 with siRNA reverses ALA's effects on testosterone biosynthesis and mitochondrial function‐related genes.ConclusionThese findings indicate that dietary supplementation with ALA can improve testosterone production in aged breeder roosters, possibly by modulation of mitochondrial function via activating the SIRT1 pathway.

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Natural Astaxanthin Improves Testosterone Synthesis and Sperm Mitochondrial Function in Aging Roosters

Cited by 8 publications

References 63 publications

Identification of Antioxidative Peptides Derived from Arthrospira maxima in the Biorefinery Process after Extraction of C-Phycocyanin and Lipids

Identification of Antioxidative Peptides Derived from Arthrospira maxima in the Biorefinery Process after Extraction of C-Phycocyanin and Lipids

Dietary Restriction Promote Sperm Remodeling in Aged Roosters Based on Transcriptome Analysis

Alpha‐Linolenic Acid Supplementation Improves Testosterone Production in an Aged Breeder Rooster Model: Role of Mitochondrial Modulation and SIRT1 Activation

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