Natural attenuation of lead by microbial manganese oxides in a karst aquifer

Newsome, Laura; Bacon, C. G. D.; Song, Huacan; Luo, Yunyao; Sherman, David M.; Lloyd, Jonathan R.

doi:10.1016/j.scitotenv.2020.142312

Cited by 13 publications

(7 citation statements)

References 95 publications

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“…The more in-depth study of the predicted EC linked to Mn metabolism showed intriguing similarities. One of the most prevalent EC predicted with similar abundances in all the samples was cytochrome-c-oxidase, linked to Mn(II) oxidation 14 . Among the others, superoxide dismutase and peroxiredoxin were also shared by all samples.…”

Section: Discussionmentioning

confidence: 94%

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Exploring structure, microbiota, and metagenome functions of epigean and hypogean black deposits by microscopic, molecular and bioinformatic approaches

Farda

Vaccarelli

Ercole

et al. 2022

Sci Rep

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This study revealed how Bacteria and Archaea communities and their metabolic functions differed between two groups of black deposits identified in gorge and cave environments. Scanning electron microscopy coupled with energy dispersive spectroscopy was used to analyse the presence of microbial biosignatures and the elemental composition of samples. Metabarcoding of the V3–V4 regions of 16S rRNA was used to investigate Bacteria and Archaea communities. Based on 16S rRNA sequencing results, PICRUSt software was used to predict metagenome functions. Micrographs showed that samples presented microbial biosignatures and microanalyses highlighted Mn concretions and layers on Al-Si surfaces. The 16S rRNA metabarcoding alpha-diversity metrics showed similar Simpson's and Shannon indices and different values of the Chao-1 index. The amplicon sequence variants (ASVs) analysis at the different taxonomic levels showed a diverse genera composition. However, the communities of all samples shared the presence of uncultured ASVs belonging to the Gemmatales family (Phylogenesis: Gemmataceae; Planctomycetes; Planctomycetota; Bacteria). The predicted metagenome functions analysis revealed diverse metabolic profiles of the Cave and Gorge groups. Genes coding for essential Mn metabolism were present in all samples. Overall, the findings on structure, microbiota, and predicted metagenome functions showed a similar microbial contribution to epigean and hypogean black deposits Mn metabolism.

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Section: Discussionmentioning

confidence: 94%

“…PICRUSt2 outputs (KO, EC, PWY) were analysed by QIIME2 software. The presence of enzymes involved in redox biotransformation, metal resistance, and respiratory processes was also verified in the EC outputs to learn more about these activities following (Supplementary Table S11 ) 14 .…”

Section: Methodsmentioning

confidence: 99%

Exploring structure, microbiota, and metagenome functions of epigean and hypogean black deposits by microscopic, molecular and bioinformatic approaches

Farda

Vaccarelli

Ercole

et al. 2022

Sci Rep

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“…On the other hand, PICRUSt2 analysis was used to explain the positive effects of enzymatic pretreatment on microbial traits involved in VFA biosynthesis. It should be noted that the PICRUSt2 has drawbacks in predicting the potential functions, although this analysis was helpful in disclosing the mechanism of enzymatic pretreatment on enhancing the fermentation performance of food waste. It was found that the organic substrates after the enzymatic pretreatment were favorable for enriching the bacteria involved in hydrolysis and acidification (e.g., Olsenella sp.…”

Section: Resultsmentioning

confidence: 99%

Enhanced Volatile Fatty Acid Production from Food Waste Fermentation via Enzymatic Pretreatment: New Insights into the Depolymerization and Microbial Traits

Zhu

et al. 2022

ACS EST Eng.

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Food waste (FW) has been considered a potential carbon reservoir and can be used for high-value volatile fatty acid (VFA) production via anaerobic fermentation. Nevertheless, more work is needed to unravel how to regulate the structural defects of FW and enhance microbial metabolisms for improving VFA production through pretreatment methods. This work investigated the enhancement of VFA production from FW fermentation through enhancing depolymerization and stimulating microbial traits via enzymatic pretreatment. The results exhibited that, after pretreatment, VFA production was improved by 50.1% compared to the unpretreated group, and the concentrations of bioavailable substrates (e.g., reducing sugar and soluble chemical oxygen demand) increased significantly. Further analysis proved that the enzymatic pretreatment could highly reduce the particle sizes of FW by 69.3% and thus promoted the solubilization of substrates and lipids. Meanwhile, the ratio of α-helix/(β-sheet + random coil) in the protein secondary structure of FW decreased from 0.32 to 0.23 after enzymatic pretreatment, indicating that the pretreatment process accelerated the FW depolymerization. Furthermore, the organic substrates after the pretreatment were favorable for enriching the bacteria involved in hydrolysis and acidification (e.g., Olsenella sp. and Sporanaerobacter sp.), upregulating the gene expressions involved in hydrolysis, pyruvate metabolism, and fatty acid biosynthesis (e.g., malZ, PK, accA, and accC), and stimulating the enzymatic activities involved in acetate and butyrate formation (e.g., AK, PTA, BK, and PTB), which was in accordance with the enhanced VFA generation from FW fermentation via enzymatic pretreatment.

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“…A study of massive deposits of Mn-oxides in a karst cave system underlying a former Pb-mine works found that they contained a diverse microbial community and had accumulated a substantial amount of Pb, up to 55 wt. % (Newsome et al, 2021). Around 5% of prokaryotic DNA sequences and 10% of fungal DNA sequences was closely related to known Mn(II)-oxidizers, and active Mn(II)-oxidising bacteria were isolated.…”

Section: Impact Of Biogeochemical Cyclingmentioning

confidence: 99%

The Microbiology of Metal Mine Waste: Bioremediation Applications and Implications for Planetary Health

Newsome

Falagán

2021

GeoHealth

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Microbes colonise and inhabit mine wastes, they tolerate high concentrations of metals and contribute to soil functioning and plant growth  Microbes transform metal speciation and environmental mobility, through metabolism, biogeochemical cycling and metal resistance mechanisms  Beneficial microbial activity can be stimulated to remediate metal-containing mine wastes, but more long-term field studies are required

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Natural attenuation of lead by microbial manganese oxides in a karst aquifer

Cited by 13 publications

References 95 publications

Exploring structure, microbiota, and metagenome functions of epigean and hypogean black deposits by microscopic, molecular and bioinformatic approaches

Exploring structure, microbiota, and metagenome functions of epigean and hypogean black deposits by microscopic, molecular and bioinformatic approaches

Enhanced Volatile Fatty Acid Production from Food Waste Fermentation via Enzymatic Pretreatment: New Insights into the Depolymerization and Microbial Traits

The Microbiology of Metal Mine Waste: Bioremediation Applications and Implications for Planetary Health

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