Natural Compounds for SIRT1-Mediated Oxidative Stress and Neuroinflammation in Stroke: A Potential Therapeutic Target in the Future

Fang, Chaoyou; Xu, Houshi; Yuan, Ling; Zhu, Zhengyang; Wang, Xiaoyu; Liu, Yibo; Zhang, Anke; Shao, Anwen; Lou, Meiqing

doi:10.1155/2022/1949718

Cited by 19 publications

(9 citation statements)

References 186 publications

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“…Central to this investigation are sirtuins, which are essential proteins implicated in cellular processes crucial for longevity and antioxidant protection [32,46,47]. Sirtuins, a family of NAD+-dependent histone deacetylase, are involved in processes like glucose and lipid metabolism [48], mitochondrial biogenesis [49], DNA repair [50], oxidative stress, apoptosis, and inflammation [51]. For this reason, sirtuins are considered key proteins in longevity control.…”

Section: Discussionmentioning

confidence: 99%

NAD-Driven Sirtuin Activation by Cordyceps sinensis Extract: Exploring the Adaptogenic Potential to Promote Skin Longevity

Di Lorenzo,

Falanga,

Ricci

et al. 2024

IJMS

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In recent years, there has been increasing interest in utilizing Traditional Chinese Medicine principles and natural bioactive compounds to combat age-related ailments and enhance longevity. A Cordyceps sinensis mycelium hydroethanolic extract (CsEx), which was standardized in cordycepin and adenosine using UHPLC-DAD, was investigated for its adaptogenic properties using in vitro assays and a double-blind, placebo-controlled clinical trial involving 40 subjects. The CsEx demonstrated activity at a concentration of 0.0006%, significantly increasing sirtuin expression (SirT1: +33%, SirT3: +10%, SirT6: +72%, vs. CTR, p < 0.05) and NAD+ synthesis in HaCat cells (+20% vs. CTR, p < 0.001). Moreover, the CsEx boosted ATP production by 68% in skin cells, correlating with higher skin energy values (+52.0% at D28, p < 0.01) in the clinical trial. Additionally, CsEx notably reduced cytosolic reactive oxygen species (ROS) by 30% in HaCaT cells (p < 0.05) and enhanced collagen production both in vitro (+69% vs. CTR, p < 0.01) and in vivo (+10% vs. D0, p < 0.01), confirmed by ultrasound examination. Furthermore, CsEx’s stimulation of fibroblasts, coupled with its antioxidant and energizing properties, led to a significant reduction in wrinkles by 28.0% (D28, p < 0.001). This study underscores Cordyceps sinensis hydroethanolic extract’s potential in regulating skin cell energy metabolism and positively influencing the mechanisms associated with skin longevity control.

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Section: Discussionmentioning

confidence: 99%

NAD-Driven Sirtuin Activation by Cordyceps sinensis Extract: Exploring the Adaptogenic Potential to Promote Skin Longevity

Di Lorenzo,

Falanga,

Ricci

et al. 2024

IJMS

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“…Sirtuins, a family of nicotinamide adenine dinucleotide (NAD + )‐dependent histone deacetylases, are critical regulators in aging‐related diseases 28,29 . SIRT1 is known as a target regulator in protecting against oxidative stress, inflammatory response, and cell apoptosis 30–32 .…”

Section: Discussionmentioning

confidence: 99%

“…Sirtuins, a family of nicotinamide adenine dinucleotide (NAD + )‐dependent histone deacetylases, are critical regulators in aging‐related diseases. 28 , 29 SIRT1 is known as a target regulator in protecting against oxidative stress, inflammatory response, and cell apoptosis. 30 , 31 , 32 According to IDD research, SIRT1 has been reported to be involved in cellular homeostasis and is a promising agent in IDD treatment.…”

Section: Discussionmentioning

confidence: 99%

Fisetin regulates the biological effects of rat nucleus pulposus mesenchymal stem cells under oxidative stress by sirtuin‐1 pathway

Zhou

Chen

Xuan

et al. 2023

Immunity Inflam & Disease

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Background Excessive oxidative stress has been accepted as one of the critical factors for intervertebral disc degeneration (IDD), which is associated with low back pain (LBP). Fisetin (Fis) is a bioactive flavonoid that possesses strong bioactive activity. In present study, we aimed to illuminate the role of Fis on nucleus pulposus mesenchymal stem cells (NPMSCs). Methods NPMSCs were isolated and cultured from rat NP tissues and identified by flow cytometry and multilinear differentiation. The cytotoxicity of Fis, EX‐527, and hydrogen peroxide (H2O2) on NPMSCs was validated using Cell Counting Kit‐8 tests. Cell apoptosis was tested by flow cytometry and TUNEL assay. Inflammatory mediators were assessed by Elisa tests, RT‐PCR. Extracellular matrix (ECM) metabolism was measured by Western blot analysis and RT‐qPCR. The expression of the SIRT1 was evaluated by Western blot analysis. Results NPMSCs were successfully isolated and cultured from rat NP tissues, and it has been identified by flow cytometry and multilinear differentiation. The results showed that Fis attenuated H2O2‐induced apoptosis, inflammation, and ECM degradation of NPMSCs. Moreover, the above protective effects of Fis can be inhibited by EX‐527, a unique SIRT1 inhibitor, indicating that SIRT1 may involve in the mechanism of Fis in protecting NPMSCs from oxidative stress. Conclusions As a natural compound with little cytotoxicity on NPMSCs, Fis alleviate H2O2‐induced apoptosis, inflammation, and ECM degradation by suppressing oxidative stress, this finding may add the theoretical basis for research on new treatment of IDD based on NPMSCs.

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“…SIRT1 and SIRT1/FOXO1 pathways also mediate the antioxidant effect of bioactive extracts containing phenolic and other natural compounds [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

SIRT1 Signaling Is Involved in the Vascular Improvement Induced by Moringa Oleifera Seeds during Aging

et al. 2023

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Vascular aging is linked to reduce NO bioavailability, endothelial dysfunction, oxidative stress, and inflammation. We previously showed that a 4-week treatment of middle-aged Wistar rats (MAWRs, 46 weeks old) with Moringa oleifera seed powder (MOI, 750 mg/kg/day) improved vascular function. Here, we investigated the involvement of SIRT1 in MOI-induced vascular improvement. MAWRs were treated with a standard or MOI-containing diet. Young rats (YWR, 16 weeks old) were the controls and received a standard diet. The hearts and aortas were harvested to evaluate SIRT1 and FOXO1 expression via Western blot and/or immunostaining, SIRT1 activity via a fluorometric assay, and oxidative stress using the DHE fluorescent probe. In the hearts and aortas, SIRT1 expression, reduced in MAWRs compared to YWRs, was enhanced in MOI MAWRs. In the hearts, SIRT1 activity did not differ between YWRs and MAWRs, whereas it was increased in MOI MAWRs compared with them. In the aortas, SIRT1 activity decreased in MAWRs, and it was similar in the MOI MAWRs and YWRs. FOXO1 expression increased in the nuclei of MAWR aortas compared to YWR and was reversed in MOI MAWRs. Interestingly, MOI treatment normalized oxidative stress enhanced in MAWRs, in both the heart and aorta. These results demonstrate the protective role of MOI against cardiovascular dysfunction due to aging via enhanced SIRT1 function and subsequently reduced oxidative stress.

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Natural Compounds for SIRT1-Mediated Oxidative Stress and Neuroinflammation in Stroke: A Potential Therapeutic Target in the Future

Cited by 19 publications

References 186 publications

NAD-Driven Sirtuin Activation by Cordyceps sinensis Extract: Exploring the Adaptogenic Potential to Promote Skin Longevity

NAD-Driven Sirtuin Activation by Cordyceps sinensis Extract: Exploring the Adaptogenic Potential to Promote Skin Longevity

Fisetin regulates the biological effects of rat nucleus pulposus mesenchymal stem cells under oxidative stress by sirtuin‐1 pathway

SIRT1 Signaling Is Involved in the Vascular Improvement Induced by Moringa Oleifera Seeds during Aging

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