Natural conduits for bridging a 15-mm nerve defect: Comparison of the vein supported by muscle and bone marrow stromal cells with a nerve autograft

Nijhuis, T.; Bodar, Corneel W.J.; Neck, Johan W. van; Walbeehm, Erik T.; Siemionow, Maria; Madajka, Maria; Cwykiel, Joanna; Blok, Joleen H.; Hovius, Steven E.R.

doi:10.1016/j.bjps.2012.09.011

Cited by 37 publications

(40 citation statements)

References 37 publications

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“…Since recovery of motor function is the most relevant postoperative achievement that is sought in the pre-clinical perspective, the most used electrophysiological method is the recording of evoked compound muscle action potentials (CMAPs) after electrical stimulation proximal and distal to the lesion site (Navarro and Udina, 2009;Nijhuis et al, 2013). As regards the sensory component, the most used method for the electrophysiological assessment is the recording of the somato-sensory evoked potentials (SSEPs) (Navarro and Udina, 2009;Chow et al, 2012) although their employment in laboratory animals has been debated (Navarro and Udina, 2009).…”

Section: Electrophysiological Assessmentmentioning

confidence: 99%

The sciatic nerve injury model in pre-clinical research

Geuna

2015

Journal of Neuroscience Methods

117

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In the pre-clinical view, the study of peripheral nerve repair and regeneration still needs to be carried out in animal models due to the structural complexity of this organ which can be only partly simulated in vitro. The far most used experimental model is based on the injury of the sciatic nerve, the largest nerve trunk in mammals. In this paper, the potential application of the sciatic nerve injury model in pre-clinical research is critically reviewed. This paper is aimed at helping researchers in properly employing this in vivo model for the study of nerve repair and regeneration as well as interpreting the results in a clinical translation perspective.

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Section: Electrophysiological Assessmentmentioning

confidence: 99%

The sciatic nerve injury model in pre-clinical research

Geuna

2015

Journal of Neuroscience Methods

117

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“…Interestingly, however, the positive effects of undifferentiated cells have also been widely reported [5,7,13,[32][33][34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51] . These investigators claim that in vitro differentiation incurs an unnecessary delay, limiting clinical applicability.…”

Section: Differentiationmentioning

confidence: 99%

“…Under appropriate conditions BMSCs can differentiate into non-mesodermal lineages such as neurons, astrocytes and SC-like cells [90] . Many studies have shown that the addition of BMSCs to conduits and acellular grafts results in superior outcomes when compared with empty or cell deplete channels [24,[36][37][38]40,41] . Although some have failed to show that BMSCs can match outcomes achieved with cultured SCs [20] , the majority of reports show that performance is at least equivalent [27,43,91] .…”

Section: Bone Marrow Derived Stem Cellsmentioning

confidence: 99%

“…Culture medium Vein graft Veins filled with uBMSCs had significantly improved functional, histological and immunohistochemical outcomes compared with veins filled with PBS Nijhuis et al [36] Rat sciatic transection (15 mm gap) U Culture medium Vein graft +/-muscle BMSC identifiable after 6 and 12-wk follow-up; vein graft + muscle + BMSCs outperformed vein graft+muscle but inferior to autograft Salomone et al [39] Rat facial nerve transection ( [28] Rat sciatic transection (10 mm gap)…”

mentioning

confidence: 99%

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Augmenting peripheral nerve regeneration using stem cells: A review of current opinion

Fairbairn

Meppelink

Ng-Glazier

et al. 2015

WJSC

128

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Outcomes following peripheral nerve injury remain frustratingly poor. The reasons for this are multifactorial, although maintaining a growth permissive environment in the distal nerve stump following repair is arguably the most important. The optimal environment for axonal regeneration relies on the synthesis and release of many biochemical mediators that are temporally and spatially regulated with a high level of incompletely understood complexity. The Schwann cell (SC) has emerged as a key player in this process. Prolonged periods of distal nerve stump denervation, characteristic of large gaps and proximal injuries, have been associated with a reduction in SC number and ability to support regenerating axons. Cell based therapy offers a potential therapy for the improvement of outcomes following peripheral nerve reconstruction. Stem cells have the potential to increase the number of SCs and prolong their ability to support regeneration. They may also have the ability to rescue and replenish populations of chromatolytic and apoptotic neurons following axotomy. Finally, they can be used in non-physiologic ways to preserve injured tissues such as denervated muscle while neuronal ingrowth has not yet occurred. Aside from stem cell type, careful consideration must be given to differentiation status, how stem cells are supported following transplantation and how they will be delivered to the site of injury. It is the aim of this article to review current opinions on the strategies of stem cell based therapy for the augmentation of peripheral nerve regeneration.

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“…It maintains neurotrophic factor secretion and cell migration and results in better outcomes [30]. Natural conduits including vein and artery grafts are rich in ECM proteins (such as collagen and laminin) and provide a good substrate for cell adhesion [29][30][31]. However, in recognition of the importance of basal lamina and other ECM framework for axonal guidance, conduits with internal structure have become more popular than hollow, single lumen tubes.…”

Section: Scaffolds and Conduitsmentioning

confidence: 99%

Recent Approaches for Augmenting Peripheral Nerve Regeneration: Mini Review

2017

MOJS

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Natural conduits for bridging a 15-mm nerve defect: Comparison of the vein supported by muscle and bone marrow stromal cells with a nerve autograft

Cited by 37 publications

References 37 publications

The sciatic nerve injury model in pre-clinical research

The sciatic nerve injury model in pre-clinical research

Augmenting peripheral nerve regeneration using stem cells: A review of current opinion

Recent Approaches for Augmenting Peripheral Nerve Regeneration: Mini Review

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