2002
DOI: 10.1159/000063886
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Natural Course of Experimental Choroidal Neovascularization: Three-Dimensional Study with Corrosion Cast and Scanning Electron Microscope

Abstract: Objectives: The details of the morphological features of choroidal neovascularization (CNV) remain unclear. The purpose of this study was to establish a CNV rat model and study the natural course of CNV using vascular casts and a scanning electron microscope (SEM). Methods: Focal laser photocoagulation (argon green 50 mW, 0.04 s, 200 µm) was applied to Brown Norway pigmented rats. Choroidal vascular casts were prepared 1 and 3 days, 1 and 2 weeks, and 1, 3 and 6 months after laser photocoagulation. The choroid… Show more

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Cited by 14 publications
(16 citation statements)
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“…However, the lCNV model has several limitations, including acute, intense laser injury to the photoreceptors, RPE, and choriocapillaris to induce angiogenesis, the accumulation of hypertrophic scar tissue, and a natural regression of the neovascularization. 6 Therefore, factors that initiate the CNV, and long-term analysis of the consequences of the neovessels to the retina, cannot be readily studied. Here we describe a novel genetic model of multifocal, bilateral spontaneous choroidal neovascularization (sCNV), which leads to early, persistent neovascular lesions that leak and lead to retinal edema, local gliosis and focal photoreceptor dysfunction, and death.…”
Section: Discussionmentioning
confidence: 99%
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“…However, the lCNV model has several limitations, including acute, intense laser injury to the photoreceptors, RPE, and choriocapillaris to induce angiogenesis, the accumulation of hypertrophic scar tissue, and a natural regression of the neovascularization. 6 Therefore, factors that initiate the CNV, and long-term analysis of the consequences of the neovessels to the retina, cannot be readily studied. Here we describe a novel genetic model of multifocal, bilateral spontaneous choroidal neovascularization (sCNV), which leads to early, persistent neovascular lesions that leak and lead to retinal edema, local gliosis and focal photoreceptor dysfunction, and death.…”
Section: Discussionmentioning
confidence: 99%
“…Unlike lCNV, which begins to retreat 7 to 10 days post laser, 6 sCNV lesions are present for at least 3 months (no observations made beyond this time point). This enables one to explore both the morphology and the consequences of advanced sCNV lesions on retinal ultrastructure and function at various time points from P40 to P90.…”
Section: Later Stages Of Scnv Associated With Edema and Focal Neuro-gmentioning
confidence: 96%
“…[40][41][42][43] Studies using laser Doppler flowmetry, 44 indocyanine green, 45,46 and fluorescein angiography 46-48 have demonstrated choroidal vascular changes that occur alongside, or precede, CNV. 40,44,46,[49][50][51][52][53][54][55] The retina, weight-forweight, has the highest oxygen demand in the body. 56 The combination of high metabolic demand and compromised choroidal supply of nutrients, and retained waste products, could well induce metabolic stress.…”
Section: Discussionmentioning
confidence: 99%
“…To establish the natural course of experimental CNV, corrosion casts were made at 1 and 3 days, 1 and 2 weeks, and 1, 3 and 6 months after laser photocoagulation [23].…”
Section: Establishment Of the Natural Course Of Cnvmentioning
confidence: 99%
“…The changes with time in choroidal corrosion casts after laser photocoagulation in nontreated rats were evaluated from 1 day after photocoagulation to 6 months after photocoagulation and have already been reported [23].…”
Section: Natural Course Of Cnvmentioning
confidence: 99%