Natural Cytotoxicity toward K562 Cells by Macaque Lymphocytes from Infancy through Puberty: Effects of Early Social Challenge

Berger, Christy L.; Boccia, Maria L.; Reite, Martin

doi:10.1006/brbi.1996.0024

Cited by 17 publications

(6 citation statements)

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“…Similarly, over a 2 week period, Marsland et al [1995] found significant correlations for numbers of circulating CD3+, CD4+, CD8+, CD19+, and CD56+ cells and for PHA-stimulated proliferation responses across some of their sampling conditions. The only longer-term study of which we are aware is a report by Laudenslager et al [1996] that described significant correlations in natural cytotoxicity among a cohort of pigtail and bonnet macaques over a 3 year period.…”

Section: Introductionmentioning

confidence: 99%

Individual differences in peripheral blood immunological and hormonal measures in adult male rhesus macaques (Macaca mulatta): Evidence for temporal and situational consistency

1998

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A growing body of research has indicated that consistent individual differences exist in physiological systems with which the immune system interacts. Few data have been reported that demonstrate stable individual differences in immunological measures, however. In the present study, enumerative measures of immune system activity were examined in 36 adult male rhesus macaques over a 13 month period under baseline conditions as well as under conditions of pharmacological and physical challenge. Blood samples were assayed for plasma concentrations of ACTH and cortisol, as well as neutrophil, total lymphocyte, CD4+ and CD8+ lymphocyte numbers, and the CD4/CD8 ratio. Analyses revealed that individual differences in the CD4/CD8 ratio and, to a lesser extent, plasma ACTH and cortisol concentrations, and neutrophil and CD4+ lymphocyte numbers were consistent across situations and times, despite changes in mean values during the various blood sampling sessions. The results suggest that the CD4/CD8 ratio might be considered trait‐like and a useful immunological measure of biobehavioral organization. Am. J. Primatol. 44:29–41, 1998. © 1998 Wiley‐Liss, Inc.

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Section: Introductionmentioning

confidence: 99%

Individual differences in peripheral blood immunological and hormonal measures in adult male rhesus macaques (Macaca mulatta): Evidence for temporal and situational consistency

1998

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“…Natural cytotoxicity was determined against K562 and Raji cell lines as previously described (Laudenslager et al, 1996). The cell lines were labeled with 51 Cr, washed in Hank’s balanced salt solution (HBSS), and resuspended to 1 × 10 5 viable cells/ml in supplemented RPMI-1640.…”

Section: Methodsmentioning

confidence: 99%

“…In young macaque monkeys between 4 and 8 months of age, a brief separation from their mother is associated with significant alterations in cell mediated and humoral immunity lasting many years (Coe, 1993; Coe and Laudenslager, 2007; Coe et al, 1988; Laudenslager et al, 1985, 1990, 1996). Health consequences also appear to be associated with these early challenges (Lewis et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

When is enough measurement, enough? Generalizability of primate immunity over time

Segerstrom

2009

Brain, Behavior, and Immunity

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A statistical generalizability analysis gauges the degree to which a single assessment of a parameter successfully estimates that measure over repeated assessments for that individual. The generalizability of enumerative and functional immune parameters was estimated for two species of macaque monkeys assessed every 3 months between 18 and 42 months of age. Subjects were crossbalanced by species (bonnet, Macaca radiata, n = 22; pigtail, Macaca nemestrina, n = 21), sex (male, n = 21; female, n = 22), and brief early maternal separation with reunion (control, n = 21; separated, n = 22). Cell subset analysis showed the best generalizability (35-69%). Natural cytotoxicity also performed well (44-70%), but when computed on a lysis per cell basis, removing the effect of cell phenotype, it was less stable (15-48%). For most immune parameters, at least 5 assessments would be necessary to establish conventionally reliable (.80) characterizations of stable individual differences in immunity, and 3 for minimally reliable (.60) characterizations. More reactive parameters, as well as more behaviorally reactive species, yielded more generalizable results. Cell subsets that are typically most sensitive to acute stress (CD8, CD16) were more stable than other subsets (CD4, CD20). Behaviorally reactive species (pigtail) yielded more stable natural cytotoxicity results than the less reactive species (bonnet). Sex and rearing condition (early, brief maternal separation) did not substantially affect generalizability, although females tended to generate more stable results than did males.

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“…Early challenges in children contribute to the etiology of posttraumatic stress disorder with significant consequences for adult behavioral and physiological regulation (Breslau, Davis, & Andreski, 1995; Hoge, Austin, & Pollack, 2007; Laudenslager. Assal, Adler, Berger, Montgomery, Sandberg, Wahlberg, Wilkins, Zweig, & Reite, 1998; Laudenslager, Berger, Boccia, & Reite, 1996). …”

Section: Introductionmentioning

confidence: 99%

The influences of perinatal challenge persist into the adolescent period in socially housed bonnet macaques (Macaca radiata)

Natvig

Corcoran

Blevins

et al. 2012

Developmental Psychobiology

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Social challenges during the perinatal period influence the mother-infant relationship in nonhuman primates and may affect the offspring's response to later social challenge(s). Relocation of a breeding colony of monkeys (Macaca radiata) created two groups of infants: one group experienced social group relocation to a new housing facility during the perinatal period (ATYPICAL) and the second group developed within a constant environment (TYPICAL). At a mean age of 25 mo., all animals were removed from their natal group and placed in same sex adolescent social groups. Behavioral observations were collected after group formation or introduction to a new group. ATYPICAL subjects showed increased aggression and reduced affiliation compared to TYPICAL subjects. Hair cortisol in male subjects collected 6 months after introduction was elevated in the ATYPICAL subjects compared to TYPICAL subjects. These findings demonstrate that early life challenges affect behavior as well as stress hormone responses to social challenge in adolescence.

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Natural Cytotoxicity toward K562 Cells by Macaque Lymphocytes from Infancy through Puberty: Effects of Early Social Challenge

Cited by 17 publications

References 0 publications

Individual differences in peripheral blood immunological and hormonal measures in adult male rhesus macaques (Macaca mulatta): Evidence for temporal and situational consistency

Individual differences in peripheral blood immunological and hormonal measures in adult male rhesus macaques (Macaca mulatta): Evidence for temporal and situational consistency

When is enough measurement, enough? Generalizability of primate immunity over time

The influences of perinatal challenge persist into the adolescent period in socially housed bonnet macaques (Macaca radiata)

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