Natural deep eutectic solvents and aqueous solutions as an alternative extraction media for propolis

Funari, Cristiano Soleo; Sutton, Adam T.; Carneiro, Renato Lajarim; Fraige, Karina; Cavalheiro, Alberto José; Bolzani, Vanderlan da Silva; Hilder, Emily F.; Arrua, R. Dario

doi:10.1016/j.foodres.2019.108559

Cited by 44 publications

(34 citation statements)

References 24 publications

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“… Trusheva et al (2019) tested the extract with choline chloride and glycerol, and it showed 8.4% of total flavones and flavonols compared to 70% ethanol (11.3%) in propolis samples of poplar type. Funari et al (2019) tested the same ES, resulting in a lower performance of phenolic compounds than 70% ethanol in green propolis samples. Some authors highlight the influence of water on the ES’s composition, showing that this may be the main factor that induces selectivity in extraction ( Liu et al, 2018 ).…”

Section: Resultsmentioning

confidence: 99%

“…The ES also has a high potential to extract bioactive compounds from natural products, including flavonoids and phenolic compounds, since they are considered as designer solvents and IL. In some cases, ES could improve the bioavailability and antioxidant activity of the extracts, which are considered as a promising characteristic for new applications, especially in the food sector ( Meng et al, 2018 ; Funari et al, 2019 ; Liu et al, 2019 ; Murador et al, 2019a ).…”

Section: Introductionmentioning

confidence: 99%

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Red Propolis as a Source of Antimicrobial Phytochemicals: Extraction Using High-Performance Alternative Solvents

et al. 2021

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Propolis is a resinous material rich in flavonoids and involved in several biological activities such as antimicrobial, fungicide, and antiparasitic functions. Conventionally, ethanolic solutions are used to obtain propolis phytochemicals, which restrict their use in some cultures. Given this, we developed an alcohol-free high-performance extractive approach to recover antibacterial and antioxidants phytochemicals from red propolis. Thus, aqueous-solutions of ionic liquids (IL) and eutectic solvents were used and then tested for their total flavonoids, antioxidant, and antimicrobial activities. The surface-responsive technique was applied regarding some variables, namely, the time of extraction, the number of extractions, and cavitation power (W), to optimize the process (in terms of higher yields of flavonoids and better antioxidant activity). After that, four extractions with the same biomass (repetitions) using 1-hexyl-3-methylimidazolium chloride [C6mim]Cl, under the operational conditions fixed at 3.3 min and 300 W, were able to recover 394.39 ± 36.30 mg RuE. g−1 of total flavonoids, with total antioxidant capacity evaluated up to 7595.77 ± 5.48 μmol TE. g−1dried biomass, besides inhibiting the growth of Staphylococcus aureus and Salmonella enteritidis bacteria (inhibition halo of 23.0 ± 1.0 and 15.7 ± 2.1, respectively). Aiming at the development of new technologies, the antimicrobial effect also presented by [C6mim]Cl may be appealing, and future studies are required to understand possible synergistic actions with propolis phytochemicals. Thereby, we successfully applied a completely alcohol-free method to obtain antimicrobials phytochemicals and highly antioxidants from red propolis, representing an optimized process to replace the conventional extracts produced until now.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Red Propolis as a Source of Antimicrobial Phytochemicals: Extraction Using High-Performance Alternative Solvents

et al. 2021

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“…Further progress has been made in the field of the utilization of natural deep eutectic solvents (NADES), which exhibit good solvent properties, while safe, of low toxicity, and from renewable resources [ 139 , 140 ]. Thus, de Funari and coworkers (2019) examined several NADES systems in propolis extractions and found that: The choline chloride (CC):1,2-PG in a molar ratio (n:n) 1:1 or 1:2; CC:lactic acid (LA):H 2 O in 1:2:2 or 1:1:1, n:n:n; are very effective ESs for propolis extraction, comparable to aqueous ethanol (70%) as a golden standard ES [ 140 ].…”

Section: Technologies For Propolis Extraction and Types Of Propolis Extractsmentioning

confidence: 99%

“…Thus, de Funari and coworkers (2019) examined several NADES systems in propolis extractions and found that: The choline chloride (CC):1,2-PG in a molar ratio (n:n) 1:1 or 1:2; CC:lactic acid (LA):H 2 O in 1:2:2 or 1:1:1, n:n:n; are very effective ESs for propolis extraction, comparable to aqueous ethanol (70%) as a golden standard ES [ 140 ]. Alternatively, aqueous solution of amino acid L -lysine (Lys; 10%) is relatively effective ES for propolis, yielding the corresponding liquid extract with about 50% of the component’s concentration obtained with aqueous EtOH (70%) [ 139 , 140 ].…”

Section: Technologies For Propolis Extraction and Types Of Propolis Extractsmentioning

confidence: 99%

Propolis Extract and Its Bioactive Compounds—From Traditional to Modern Extraction Technologies

Šuran

Cepanec²,

Mašek

et al. 2021

Molecules

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Propolis is a honeybee product known for its antioxidant, anti-inflammatory, anticancer, and antimicrobial effects. It is rich in bioactive molecules whose content varies depending on the botanical and geographical origin of propolis. These bioactive molecules have been studied individually and as a part of propolis extracts, as they can be used as representative markers for propolis standardization. Here, we compare the pharmacological effects of representative polyphenols and whole propolis extracts. Based on the literature data, polyphenols and extracts act by suppressing similar targets, from pro-inflammatory TNF/NF-κB to the pro-proliferative MAPK/ERK pathway. In addition, they activate similar antioxidant mechanisms of action, like Nrf2-ARE intracellular antioxidant pathway, and they all have antimicrobial activity. These similarities do not imply that we should attribute the action of propolis solely to the most representative compounds. Moreover, its pharmacological effects will depend on the efficacy of these compounds’ extraction. Thus, we also give an overview of different propolis extraction technologies, from traditional to modern ones, which are environmentally friendlier. These technologies belong to an open research area that needs further effective solutions in terms of well-standardized liquid and solid extracts, which would be reliable in their pharmacological effects, environmentally friendly, and sustainable for production.

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“…Ethanolic extracts of propolis are consumed for their health benefits. Owing to the biocompatibility, biodegradability, and renewability of NADESs, they were used to extract propolis to substitute alcohol [43]. Sixteen NADESs prepared from non-toxic materials (e.g., essential amino acids) were successfully used for the green extraction of propolis including its biomarker artepillin C. Most importantly, the extracts of propolis based on NADES were much more stable over the months and could be consumed directly with no need for the separation of the bioactive compounds from NADES.…”

Section: Dess For the Extraction Of Other Polar Organic Compoundsmentioning

confidence: 99%

Application of Deep Eutectic Solvents in Food Analysis: A Review

Chen

Wang

et al. 2019

Molecules

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Deep eutectic solvents (DESs) have emerged recently as new and green solvents. DESs can be used for extraction and separation of both inorganic metallic components and organic compounds such as phenolic compounds, flavonoids, sugars, and aromatic amines from food samples. DESs possess a tunable property simply by adjusting the ratio of hydrogen bond acceptors to the hydrogen bond donors. As a green extraction medium, DESs have various applications in the pretreatment process and improve the efficiency of different food analyses. This review summarizes the findings of recent studies on the development, production, application, and efficacy of DESs in the pretreatment process of various food analyses.

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Natural deep eutectic solvents and aqueous solutions as an alternative extraction media for propolis

Cited by 44 publications

References 24 publications

Red Propolis as a Source of Antimicrobial Phytochemicals: Extraction Using High-Performance Alternative Solvents

Red Propolis as a Source of Antimicrobial Phytochemicals: Extraction Using High-Performance Alternative Solvents

Propolis Extract and Its Bioactive Compounds—From Traditional to Modern Extraction Technologies

Application of Deep Eutectic Solvents in Food Analysis: A Review

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