Natural deep eutectic solvents protect RNA from thermal-induced degradation

Mattioli, Roberto; Maggiore, Anna; Risola, Daniel Di; Federico, Rodolfo; D’Erme, Maria; Francioso, Antonio; Mosca, Luciana

doi:10.1016/j.abb.2023.109714

Archives of Biochemistry and Biophysics

2023

DOI: 10.1016/j.abb.2023.109714

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Natural deep eutectic solvents protect RNA from thermal-induced degradation

Roberto Mattioli

Anna Maggiore

Daniel Di Risola

et al.

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2024

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Cited by 2 publications

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Developing a Ready-to-Use Lipid Nanoparticle Technology for Nucleic Acid Delivery Based on Deep Eutectic Solvents

Wei,

Kong,

Dong

et al. 2024

Nano Lett.

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Microfluidic technology has emerged as a prevalent tool to produce lipid nanoparticles (LNPs) for nucleic acid delivery. However, its wide-ranging application is hindered by specialized, costly equipment and consumables. Herein, a ready-touse lipid nanoparticle (RULNP) technology employing deep eutectic solvents (DESs) was developed. The DES, consisting of fructose and glycerol ([Fru][Gly]), was able to dissolve lipids and nucleic acids, facilitating the formation of RULNPs by simple physical mixing and hydrating. This innovative approach circumvents the high costs and organic solvents associated with microfluidic methods and offers flexibility in preparation techniques, accommodating various application scenarios. RULNPs exhibited physicochemical properties and plasmid DNA (pDNA) or RNA delivery efficacy comparable to those of LNPs. Mechanistic studies revealed that RULNPs achieved superior cellular uptake compared with LNPs despite exhibiting limited endosomal escape capabilities. Collectively, the DES-based RULNP system presents a rapid and straightforward method for LNP production, potentially revolutionizing nucleic acid delivery.

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Developing a Ready-to-Use Lipid Nanoparticle Technology for Nucleic Acid Delivery Based on Deep Eutectic Solvents

Wei,

Kong,

Dong

et al. 2024

Nano Lett.

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Theoretical Model for In Vivo Induction of Chemotherapy Sensitization Using miRNA Packaged in Distinct Layered Liposomes

Cipu,

Stănişteanu,

Andrei

et al. 2024

JFB

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Resistance to chemotherapy is a problem of major social and economic importance, when looking at factors like the decrease in life expectancy, the associated therapeutic costs, and a significant number of cancers that resist current chemotherapy. The development of chemotherapeutics for all theoretically possible tumor variants is an approach that requires unreasonable resources. We propose a theoretical model that serves the purpose of overcoming resistance to chemotherapeutic agents used in cancer therapy. The model describes a gene delivery system based on liposomes, which are optically guided to the tumor’s location. The main aim of the gene delivery system is inhibiting the activity of enzymes involved in drug metabolism, hence offering the opportunity to use inexpensive chemotherapeutics that are already on the market. This model will reduce the costs of chemotherapy and will assure a positive outcome for patients.

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Natural deep eutectic solvents protect RNA from thermal-induced degradation

Cited by 2 publications

References 25 publications

Developing a Ready-to-Use Lipid Nanoparticle Technology for Nucleic Acid Delivery Based on Deep Eutectic Solvents

Developing a Ready-to-Use Lipid Nanoparticle Technology for Nucleic Acid Delivery Based on Deep Eutectic Solvents

Theoretical Model for In Vivo Induction of Chemotherapy Sensitization Using miRNA Packaged in Distinct Layered Liposomes

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