Natural enzyme-free colorimetric immunoassay for human chorionic gonadotropin detection based on the Ag+-triggered catalytic activity of cetyltrimethylammonium bromide-coated gold nanoparticles

Guo, Yuqian; Zhou, Yaofeng; Xiong, Sicheng; Zeng, Linjuan; Huang, Xiaolin; Leng, Yuankui; Xiong, Yonghua

doi:10.1016/j.snb.2019.127439

Cited by 20 publications

(13 citation statements)

References 31 publications

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“…The limit of detection (LOD, S/N = 3) for target hCG was calculated to be 0.19 mIU/mL. Compared with sensitive hCG biosensors developed previously, − the fabricated peptide-based photocathodic biosensor had a comparable or even lower LOD.…”

Section: Resultsmentioning

confidence: 92%

Peptide-Based Photocathodic Biosensors: Integrating a Recognition Peptide with an Antifouling Peptide

Shi

Zhang

et al. 2021

Anal. Chem.

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Accurate and sensitive detection of targets in practical biological matrixes such as blood, plasma, serum, or tissue fluid is a frontier issue for most biosensors since the coexistence of both potential reducing agents and protein molecules has the possibility of causing signal interference. Herein, aiming at detection in a complex environment, an advanced and robust peptide-based photocathodic biosensor, which integrated a recognition peptide with an antifouling peptide in one probe electrode, was first proposed. Selecting human chorionic gonadotropin (hCG) as a model target, the recognition peptide with the sequence PPLRINRHILTR was first anchored on the CuBi2O4/Au (CBO/Au) photocathode and then the antifouling peptide with the sequence EKEKEKEPPPPC was further anchored to generate an antifouling biointerface. The peptide-based photocathodic biosensor demonstrated excellent anti-interference to both nonspecific proteins and reducing agents because of the capability of the antifouling peptide. It also exhibited good sensitivity owing to the utilization of the recognition peptide rather than an antibody probe. This peptide-integrated method offers a new perspective for practical applications of photocathodic biosensors.

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Section: Resultsmentioning

confidence: 92%

Peptide-Based Photocathodic Biosensors: Integrating a Recognition Peptide with an Antifouling Peptide

Shi

Zhang

et al. 2021

Anal. Chem.

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“…It is reported that silver ions can regulate the catalytic activities of some nanozymes (such as AgNPs) [ 34 , 40 , 88 , 89 ]. Khoris et.…”

Section: Immunoassay Based On Nanozymesmentioning

confidence: 99%

“…Ag + can significantly improve the oxidation ability of TMB to achieve signal amplification. Guo and co-workers utilized silver ions to activate the mimic peroxidase activity of cetyltrimethylammonium bromide (CTAB)-coated gold nanoparticles (AuNPs@CTAB) and used AuNPs@CTAB with Ag + -triggered catalytic activity as the signal sensor to construct a colorimetric immunoassay for the detection of human chorionic gonadotropin (HCG) [ 40 ]. Abundant Ag + were released through the corrosion of silver nanoparticles by hydrogen peroxide.…”

Section: Immunoassay Based On Nanozymesmentioning

confidence: 99%

Recent Advances in the Immunoassays Based on Nanozymes

et al. 2022

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As a rapid and simple method for the detection of multiple targets, immunoassay has attracted extensive attention due to the merits of high specificity and sensitivity. Notably, enzyme-linked immunosorbent assay (ELISA) is a widely used immunoassay, which can provide high detection sensitivity since the enzyme labels can promote the generation of catalytically amplified readouts. However, the natural enzyme labels usually suffer from low stability, high cost, and difficult storage. Inspired by the advantages of superior and tunable catalytic activities, easy preparation, low cost, and high stability, nanozymes have arisen to replace the natural enzymes in immunoassay; they also possess equivalent sensitivity and selectivity, as well as robustness. Up to now, various kinds of nanozymes, including mimic peroxidase, oxidase, and phosphatase, have been incorporated to construct immunosensors. Herein, the development of immunoassays based on nanozymes with various types of detection signals are highlighted and discussed in detail. Furthermore, the challenges and perspectives of the design of novel nanozymes for widespread applications are discussed.

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“…Inspired by the idea of “old drug in new use,” a number of well-developed and widely used techniques have been employed for SARS-CoV-2 detection. Typical representatives of these analytical technologies include enzyme-linked immunosorbent assay (ELISA) [ 14 ], lateral flow assay (LFA) [ 15 ], biosensors, and isothermal amplification-assisted nucleic acid test [ 16 , 17 ]. LFA has obtained a great success in COVID-19 diagnosis because it well matches the ASSURED criteria of the WHO for POC testing [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Point-of-care COVID-19 diagnostics powered by lateral flow assay

Zhou

Ding

et al. 2021

TrAC Trends in Analytical Chemistry

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136

118

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Since its first discovery in December 2019, the global coronavirus disease 2019 (COVID-19) pandemic caused by the novel coronavirus (SARS-CoV-2) has been posing a serious threat to human life and health. Diagnostic testing is critical for the control and management of the COVID-19 pandemic. In particular, diagnostic testing at the point of care (POC) has been widely accepted as part of the post restriction COVID-19 control strategy. Lateral flow assay (LFA) is a popular POC diagnostic platform that plays an important role in controlling the COVID-19 pandemic in industrialized countries and resource-limited settings. Numerous pioneering studies on the design and development of diverse LFA-based diagnostic technologies for the rapid diagnosis of COVID-19 have been done and reported by researchers. Hundreds of LFA-based diagnostic prototypes have sprung up, some of which have been developed into commercial test kits for the rapid diagnosis of COVID-19. In this review, we summarize the crucial role of rapid diagnostic tests using LFA in targeting SARS-CoV-2-specific RNA, antibodies, antigens, and whole virus. Then, we discuss the design principle and working mechanisms of these available LFA methods, emphasizing their clinical diagnostic efficiency. Ultimately, we elaborate the challenges of current LFA diagnostics for COVID-19 and highlight the need for continuous improvement in rapid diagnostic tests.

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Natural enzyme-free colorimetric immunoassay for human chorionic gonadotropin detection based on the Ag+-triggered catalytic activity of cetyltrimethylammonium bromide-coated gold nanoparticles

Cited by 20 publications

References 31 publications

Peptide-Based Photocathodic Biosensors: Integrating a Recognition Peptide with an Antifouling Peptide

Peptide-Based Photocathodic Biosensors: Integrating a Recognition Peptide with an Antifouling Peptide

Recent Advances in the Immunoassays Based on Nanozymes

Point-of-care COVID-19 diagnostics powered by lateral flow assay

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