Natural fluorescence of white blood cells: spectroscopic and imaging study

Monici, Monica; Pratesi, Riccardo; Bernabei, Pietro Antonio; Caporale, Roberto; Ferrini, Pierluigi Rossi; Croce, Anna Cleta; Balzarini, Piera; Bottiroli, Giovanni

doi:10.1016/1011-1344(95)07149-v

Cited by 71 publications

(43 citation statements)

References 20 publications

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“…A number of studies have shown that there is a correlation between the 295 nm excitation band and epidermal proliferation (Kollias et al, 1998;Brancaleon et al, 1999Brancaleon et al, , 2001Gillies et al, 2000) and increased cellular activity in vitro (Zhang et al, 1997). Monici et al (1995) have observed an excitation band at 270 nm associated with inflammation. It is not known, however, whether the 278 nm band measured in this study is the same with the 270 nm band reported in the literature (Le et al, 1995(Le et al, , 1998.…”

Section: Figurementioning

confidence: 97%

Non-Invasive Evaluation of the Kinetics of Allergic and Irritant Contact Dermatitis

Astner

González

Cheung

et al. 2005

Journal of Investigative Dermatology

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Reflectance confocal microscopy (RCM) allows non-invasive visualization of human skin in vivo. It has been used to describe the histopathological features of acute contact dermatitis (CD). This work was designed to investigate the kinetics of both allergic and irritant CD (ACD and ICD) in vivo. Eighteen subjects with a prior diagnosis of ACD were patch tested with the specific allergen sodium lauryl sulfate as an irritant, and appropriate controls. RCM, transepidermal water loss (TEWL), and fluorescence excitation spectroscopy (FES) were performed at several time points within 2 wk after patch removal. After removal of the Finn chambers at 48 h, superficial epidermal changes, primarily involving the stratum corneum, and increased epidermal thickness were mainly present in ICD. ACD, on the other hand, showed microvesicle formation peaking at 96 h following patch removal. Both ACD and ICD showed exocytosis and similar degrees of spongiosis on RCM. TEWL and FES demonstrated a significant difference between ACD and ICD. RCM, TEWL, and FES are valuable non-invasive tools to quantitatively study the kinetics of the pathophysiology of acute CD reactions in vivo and monitor the changes at a cellular level.

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Section: Figurementioning

confidence: 97%

Non-Invasive Evaluation of the Kinetics of Allergic and Irritant Contact Dermatitis

Astner

González

Cheung

et al. 2005

Journal of Investigative Dermatology

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“…8,9 The other fluorophores, pyridoxine derivatives, NADH, FAD, and endogenous porphyrin are related to metabolic processes. 10,11 A change in the state of the tissue such as that occurring during physiological processes or in connection with the onset of pathological conditions results in modifications of the amount of fluorophores, their distribution, and the biochemical properties of their environment. 5 Several groups have reported the use of fluorescence spectroscopy for the characterization of various types of cancers, such as those of the cervix, [12][13][14] oral area, [15][16][17] colon, 18,19 and breast.…”

Section: Introductionmentioning

confidence: 99%

Synchronous fluorescence spectroscopic characterization of DMBA-TPA-induced squamous cell carcinoma in mice

Diagaradjane

Yaseen

et al. 2006

J. Biomed. Opt.

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While initially confined to the epidermis, squamous cell carcinoma can eventually penetrate into the underlying tissue if not diagnosed early and treated. The noninvasive early detection of the carcinoma is important to achieve a complete treatment of the disease. Of the various non-invasive optical techniques, the synchronous fluorescence (SF) technique is considered to provide a simplified spectral profile with more sharp spectral signatures of the endogenous fluorophores in complex systems. The potential use of the SF technique in the characterization of the sequential tissue transformation in 7,12-dimethylbenz(a)anthracene-12-O-tetradecanoylphorbol-13-acetate (DMBA-TPA)-induced mouse skin tumor model in conjunction with simple statistical analysis is explored. The SF spectra show distinct differences during the earlier weeks of the tumor-induction period. Intensity ratio variables are calculated and used in three discriminant analyses. All the discriminant analyses show better classification results with accuracy greater than 80%. From the observed differences in the spectral characteristics and the ratio variables that resulted in better classification between groups, it is concluded that tryptophan, collagen, and NADH are the key fluorophores that undergo changes during tissue transformation process and hence they can be targeted as tumor markers to diagnose normal from abnormal tissues using the SF technique.

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“…Progress by Monici and Heintzelman has also been made characterizing the autofluorescence of white blood cells, though the clinical applications are not yet as apparent. (19, 20)…”

Section: Introductionmentioning

confidence: 99%

Differentiation of Malignant B-Lymphoma Cells from Normal and Activated T-Cell Populations by Their Intrinsic Autofluorescence

Pantanelli

Li²,

Fariss³

et al. 2009

Cancer Research

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Natural fluorescence of white blood cells: spectroscopic and imaging study

Cited by 71 publications

References 20 publications

Non-Invasive Evaluation of the Kinetics of Allergic and Irritant Contact Dermatitis

Non-Invasive Evaluation of the Kinetics of Allergic and Irritant Contact Dermatitis

Synchronous fluorescence spectroscopic characterization of DMBA-TPA-induced squamous cell carcinoma in mice

Differentiation of Malignant B-Lymphoma Cells from Normal and Activated T-Cell Populations by Their Intrinsic Autofluorescence

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