Natural Fluorescence Spectroscopy of Human Blood Plasma in the Diagnosis of Colorectal Cancer: Feasibility Study and Preliminary Results

Lualdi, Manuela; Colombo, Ambrogio; Leo, Ermanno; Morelli, Daniele; Vannelli, Alberto; Battaglia, Luigi; Marchesini, Renato

doi:10.1177/030089160709300609

Cited by 36 publications

(29 citation statements)

References 15 publications

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“…Pathologic tissue changes cause an alteration of the histologic organization and changes in biochemical and physiochemical properties mainly due to collagen, elastin, nicotinamide adenine dinucleotide, porphyrin and flavin adenine dinucleotide (FAD) composition, which are reflected in an altered fluorescence emission spectrum. 10 Near-infrared spectroscopy uses the absorption profiles of DNA/RNA, carbohydrates, lipids, proteins, and water to provide information about molecular composition and the biological profile of tissues. 8 Both optical spectroscopy methods have shown a great potential in the discrimination of malignant and benign tissue and have been applied in colorectal, breast, prostate, and lung cancer research.…”

Section: Introductionmentioning

confidence: 99%

Synergy of Fluorescence and Near-Infrared Spectroscopy in Detection of Colorectal Cancer

Ehlen

Zabaryło

Speichinger

et al. 2019

Journal of Surgical Research

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Magnetic resonance spectroscopy a b s t r a c tBackground: Colorectal cancer is one of the most common malignancies worldwide. There is an urgent need for simple and fast methods to improve tumor detection in the diagnostic and intraoperative setting to avoid complications and provide objective information in distinguishing malignant and benign colorectal tissue. Optical spectroscopy methods have recently shown a great potential for this discrimination in different organs. Materials and methods:In this pilot study, fluorescence emission spectra (excitation: 473 nm) and diffuse reflectance spectra (DRS) of normal and tumor tissues from resected colorectal cancer specimen were measured using fiber optical probes in an ex vivo setting, and the data were subjected to multivariate analysis.Results: Substantial spectral differences were found in the fluorescence and DRS spectra of colorectal cancer tissue in comparison to benign tissue. The diagnostic potential of a multimode optical system combining both spectroscopic methods was investigated by mathematical combination. Compared with the individual techniques, a higher sensitivity of the joint DRS-fluorescence optical system in the discrimination between malignant and benign colorectal tissue could be observed. Conclusions:In the pilot study presented herein, a quick and reliable method to differentiate malignant and benign colorectal tissue ex vivo with different spectroscopic techniques using spectral fiber probes could be established. Joint fluorescence and near-infrared spectroscopy had a higher sensitivity in tissue discrimination and showed to be a promising combination of two spectroscopic methods. Further studies using the synergic effect of fluorescence and DRS spectroscopy are needed to transfer these findings into the in vivo situation. ª

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Section: Introductionmentioning

confidence: 99%

Synergy of Fluorescence and Near-Infrared Spectroscopy in Detection of Colorectal Cancer

Ehlen

Zabaryło

Speichinger

et al. 2019

Journal of Surgical Research

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“…Algorithms such as principal components analysis (PCA) [12], linear discriminant analysis (LDA) [13], and partial least-squares (PLS) [14] were often used combined with those spectral features. But those parameters can only provide limited information about the whole spectra, neglecting others such as fluorescence background which has been used on the blood for the diagnosis of several cancers [6,15]. Richer information will be obtained using both the features of Raman and the background fluorescence spectroscopy.…”

Section: Introductionmentioning

confidence: 99%

Discrimination of serum Raman spectroscopy between normal and colorectal cancer using selected parameters and regression-discriminant analysis

Yang

2012

Appl. Opt.

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Raman spectroscopy of tissues has been widely studied for the diagnosis of various cancers, but biofluids were seldom chosen as the analyte because of the low concentration. Herein, serum of 30 normal people, 46 colon cancer, and 44 rectum cancer patients were measured using Raman spectra and analyzed. The information of Raman peaks (intensity and width) and that of the fluorescence background (baseline function coefficients) were selected as parameters for statistical analysis. Principal component regression (PCR) and partial least square regression (PLSR) were used on the selected parameters separately to see the diagnosing performance of the parameters. PCR performed better than PLSR in our spectral data. Then linear discriminant analysis (LDA) was used on the principal components (PCs) of the two regression methods on the selected parameters, and the diagnostic accuracy were 88% and 83%. The conclusion is that the selected parameters can maintain the information of the original spectra well and Raman spectroscopy of serum has the potential for the diagnosis of colorectal cancer.

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“…Several studies had been reported on the native fluorescence spectroscopy of various tissues (2,3,(29)(30)(31). As any metabolic changes in the body may reflect in the body fluids, attempts have also been made on the characterization of body biofluids, viz., blood in diagnosis of liver diseases and cancer (1,14). It has also been reported that urine is a diagnostically important biofluids, which has many metabolites.…”

Section: Discussionmentioning

confidence: 99%

“…Although many applications of native fluorescence spectroscopy of biomolecules are reported on the characterization of cellular metabolic pathways and the discrimination of malignant from normal conditions of tissues, only limited studies have been reported on the applications of native fluorescence spectroscopy of biofluids in diagnostic oncology. Only limited data are available on the optical spectroscopic characterization of body biofluids such as blood (1,(12)(13)(14), saliva (15,16) and urine (17,18). Although Leiner et al has discussed the possible shoulder emission spectra of various molecules at different excitation and emission ranges, they have not compared cancer samples with normal urine samples (17).…”

Section: Introductionmentioning

confidence: 99%

Characterization and Diagnosis of Cancer by Native Fluorescence Spectroscopy of Human Urine

Rajasekaran

Aruna

Koteeswaran

et al. 2012

Photochem & Photobiology

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Urine is one of the diagnostically important bio fluids, as it has different metabolites in it, where many of them are native fluorophores. Native fluorescence characteristics of human urine samples were studied using excitation-emission matrices (EEMs) over a range of excitation and emission wavelengths, and emission spectra at 405 nm excitation, to discriminate patients with cancer from the normal subjects. The fluorescence spectra of urine samples of cancer patients exhibit considerable spectral differences in both EEMs and emission spectra with respect to normal subjects. Different ratios were calculated using the fluorescence intensity values of the emission spectra and they were used as input variables for a multiple linear discriminant analysis across different groups. The discriminant analysis classifies 94.7% of the original grouped cases and 94.1% of the cross-validated grouped cases correctly. Based on the fluorescence emission characteristics of urine and statistical analysis, it may be concluded that the fluorophores nicotinamide adenine dinucleotide (NADH) and flavins may be considered as metabolomic markers of cancer.

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Natural Fluorescence Spectroscopy of Human Blood Plasma in the Diagnosis of Colorectal Cancer: Feasibility Study and Preliminary Results

Abstract: These results, although preliminary, suggest the potential of fluorescence measurements of blood plasma as an additional method for diagnostic application in colon cancer.

Cited by 36 publications

References 15 publications

Synergy of Fluorescence and Near-Infrared Spectroscopy in Detection of Colorectal Cancer

Synergy of Fluorescence and Near-Infrared Spectroscopy in Detection of Colorectal Cancer

Discrimination of serum Raman spectroscopy between normal and colorectal cancer using selected parameters and regression-discriminant analysis

Characterization and Diagnosis of Cancer by Native Fluorescence Spectroscopy of Human Urine

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