To determine the ecological and hygienic status of the environment and its possible impact on population health, the issue of developing a comprehensive methodology for integrated assessment of the environment condition, in particular, at the regional level, has been set. The research objective was to develop the methodology for ecological and hygienic integrated assessment of the environment and its individual indicators, and to test it at the separate regional level of the Russian Federation-theVladimirregion,primarilyregardingthehumanlifequality. TheresearchisbasedontheofficialstatisticsdatapublishedbytheVladimir region administration, “Federal hygiene and epidemiology center of the Vladimir region” for the period of 2001-2015, as well as our own research oftheregionalenvironmentcondition(qualityofair,municipalwatersupply and soil). As the research result, the statistics data of the State offices engaged in social and hygienic monitoring, as well as own environmental surveys, characterizing the quality of atmospheric air, municipal water supply and soil in residential areas were analyzed, and relatively safe and relatively unfavorable environmental areas were identified. The environmental quality analysis and the application of the integrated assessmentmethodologymadeitpossibletoranktheregionareasaccording to the ecological and hygienic well-beingrate