Natural food flavour (E)-2-hexenal, a potential antifungal agent, induces mitochondria-mediated apoptosis in Aspergillus flavus conidia via a ROS-dependent pathway

Ma, Weibin; Zhao, Luling; Johnson, Eric T.; Xie, Yanli; Zhang, Mingming

doi:10.1016/j.ijfoodmicro.2022.109633

Cited by 25 publications

(7 citation statements)

References 53 publications

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“…Second line defense antioxidants, such as glutathione, act as scavengers of free radicals . In recent years, numerous natural antifungal compounds have been discovered, such as thymol and salicylic acid, nerolidol, terpinen-4-ol, linalool, and ( E )-2-hexenal . These compounds stimulate ROS production, which in turn causes damage to fungal cells.…”

Section: Discussionmentioning

confidence: 99%

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ROS Stress and Cell Membrane Disruption are the Main Antifungal Mechanisms of 2-Phenylethanol against Botrytis cinerea

Zou

Wei

Jiang

et al. 2022

J. Agric. Food Chem.

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2-Phenylethanol (2-PE), a common compound found in plants and microorganisms, exhibits broad-spectrum antifungal activity. Using Botrytis cinerea, we demonstrated that 2-PE suppressed mycelium growth in vitro and in strawberry fruit and reduced natural disease without adverse effects to fruit quality. 2-PE caused structural damage to mycelia, as shown by scanning and transmission electron microscopy. From RNA sequencing analysis we found significantly upregulated genes for enzymatic and nonenzymatic reactive oxygen species (ROS) scavenging systems including sulfur metabolism and glutathione metabolism, indicating that ROS stress was induced by 2-PE. This was consistent with results from assays demonstrating an increase ROS and hydrogen peroxide levels, antioxidant enzyme activities, and malondialdehyde content in treated cells. The upregulation of ATP-binding cassette transporter genes, the downregulation of major facilitator superfamily transporters genes, and the downregulation of ergosterol biosynthesis genes indicated a severe disruption of cell membrane structure and function. This was consistent with results from assays demonstrating compromised membrane integrity and lipid peroxidation. To summarize, 2-PE exposure suppressed B. cinerea growth through ROS stress and cell membrane disruption.

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Section: Discussionmentioning

confidence: 99%

“…27 In recent years, numerous natural antifungal compounds have been discovered, such as thymol and salicylic acid, 28 nerolidol, 29 terpinen-4-ol, 21 linalool, 30 and (E)-2-hexenal. 31 These compounds stimulate ROS production, which in turn causes damage to fungal cells. 2-PE was found to rapidly induce ROS accumulation of K. marxianus.…”

Section: Discussionmentioning

confidence: 99%

ROS Stress and Cell Membrane Disruption are the Main Antifungal Mechanisms of 2-Phenylethanol against Botrytis cinerea

Zou

Wei

Jiang

et al. 2022

J. Agric. Food Chem.

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“…The mitochondrial membrane potential is an important element of ion motive force. It is involved in the generation of ATP and metabolic activities in cells and the decrease of the mitochondrial membrane potential is the characteristic mark of apoptosis [ 34 , 35 ]. PEO may inhibit pellicle formation in Sichuan pickles by affecting the cell membrane, increasing membrane permeability and damaging the mitochondrial membrane potential.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Perilla frutescens Essential Oil on Pellicle Formation of Candida tropicalis and Pichia kluyveri and Its Effect on Volatile Compounds in Sichuan Pickles

Cai

Shi

Zhang

et al. 2023

Foods

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Pellicle formation is the most typical characteristic of deteriorating fermented vegetable products. Perilla frutescens essential oil (PEO) is widely used as a useful natural preservative. However, few studies have addressed the antifungal activity and mechanism of PEO in pellicle formation microorganisms, and it is still unclear whether it can inhibit pellicle formation and affect its volatile compounds in Sichuan pickles. The current study showed that PEO can inhibit pellicle formation during fermentation of Sichuan pickles as it had significant antifungal activity against the pellicle formation microorganisms Candida tropicalis SH1 and Pichia kluyveri SH2. The minimum inhibitory concentration (MIC) of PEO against C. tropicalis SH1 and P. kluyveri SH2 was determined to be 0.4 μL/mL, and the minimum fungicidal concentrations (MFCs) were 1.6 μL/mL and 0.8 μL/mL, respectively. The antifungal mechanism was activated as a result of damage to the cell membrane, an increase in the cell permeability, a decrease in the mitochondrial membrane potential, and the inhibition of ATPase activity. Meanwhile, the addition of PEO to Sichuan pickles can enrich the profiles of volatile compounds during fermentation, including limonene, myrcene, 1,8-cineole, linalool, perilla ketone, heptanal, hexanal, α-thujone and β-terpineol and thus improve the overall sensory acceptability. These results indicated that PEO has the potential to be used as a novel food preservative to control pellicle formation in fermented vegetables.

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“…The intracellular ROS realease was measured by flow cytometer ( Ye et al, 2021 ; Ma et al, 2022 ). Briefly, A549 cells were inoculated in 12-well plates (1 × 10 5 cells per well) and cultured under appropriate conditions (5% CO 2 , 37°C) for 24 h. Then, A549 cells were treated with cratoxylumxanthone C (7.5, 15, and 30 μ M) for 48 h. Then, the cells were collected, washed with PBS, and stained with 10 μ M DCFH-DA probe (Beyotime, Shanghai, China) for 20 min at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Cratoxylumxanthone C, a natural xanthone, inhibits lung cancer proliferation and metastasis by regulating STAT3 and FAK signal pathways

Wang

Liu

et al. 2022

Front. Pharmacol.

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To discover phytochemicals as lead compounds for cancer treatment, cratoxylumxanthone C, a natural xanthone, was obtained from Cratoxylum cochinchinense (Lour.) Bl., for which there have been no reports on the biological effects against cancer. Our study revealed that cratoxylumxanthone C had significant anti-tumor activity by inducing apoptosis, augmenting cellular reactive oxygen species (ROS), and arresting cell circle. The mechanistic examination showed the inhibition of A549 cell proliferation and metastasis by cratoxylumxanthone C was coupled with the signal transducer and activator of transcription 3 (STAT3) and focal adhesion kinase (FAK) signaling pathways. Furthermore, the zebrafish models confirmed its significant in vivo anti-tumor activity, in which cratoxylumxanthone C inhibited tumor proliferation and metastasis and suppressed the angiogenesis. Comprehensively, these cellular and zebrafish experiments implied that cratoxylumxanthone C may have the potential to become an anti-tumor agent for lung cancer, especially non-small cell lung cancer (NSCLC).

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Natural food flavour (E)-2-hexenal, a potential antifungal agent, induces mitochondria-mediated apoptosis in Aspergillus flavus conidia via a ROS-dependent pathway

Cited by 25 publications

References 53 publications

ROS Stress and Cell Membrane Disruption are the Main Antifungal Mechanisms of 2-Phenylethanol against Botrytis cinerea

ROS Stress and Cell Membrane Disruption are the Main Antifungal Mechanisms of 2-Phenylethanol against Botrytis cinerea

Inhibition of Perilla frutescens Essential Oil on Pellicle Formation of Candida tropicalis and Pichia kluyveri and Its Effect on Volatile Compounds in Sichuan Pickles

Cratoxylumxanthone C, a natural xanthone, inhibits lung cancer proliferation and metastasis by regulating STAT3 and FAK signal pathways

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