Natural Garlic Organosulfur Compounds Prevent Metabolic Disorder of Lipid and Glucose by Increasing Gut Commensal Bacteroides acidifaciens

Abstract

A number of reports of the effects of garlic on gut microbiota revealed that the active garlic organosulfur compounds (OSCs) are destabilized by the action of alliinase during garlic preparation. In this study, garlic alliinase was deactivated to obtain stable garlic OSCs. Experiments with C57BL/6J mice fed with lipid and glucose metabolic disorder-inducing Western diet (WD) revealed that stable garlic OSCs prevented the disorder by increasing the relative abundance of gut Bacteroides acidifaciens. Molecular analysis indicated that garlic OSCs inhibited dyslipidemia and fatty liver by increasing taurine and subsequently promoting hepatic fatty acid β-oxidation. In parallel, garlic OSCs could meliorate glucose homeostasis by inhibiting dipeptidyl peptidase-4 (DPP-4) and hepatic gluconeogenesis. In vitro bacterial culture experiments revealed that garlic OSCs directly increased the growth of gut Bacteroides acidifaciens. The results of this study demonstrate that the molecular mechanism of the preventive effect of garlic OSCs on the WD-induced metabolic disorder is attributed to the enhanced growth of Bacteroides acidifaciens and the consequent increase in taurine.

Diallyl trisulfide inhibits 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone-induced lung cancer via modulating gut microbiota and the PPARγ/NF-κB pathway

“…37 Garlic, a natural dietary source of H 2 S, 38 has been investigated to regulate gut microbiome and ameliorate various diseases, including hypertension, 39 atherosclerosis, 40 and metabolic disorders. 41 DATS, as one of the main active components of garlic, may, therefore, modulate the gut microbiota and play a crucial role in preventing lung cancer. To investigate this hypothesis, we performed 16S rRNA sequencing analysis to elucidate the composition of the gut microflora in mice treated with DATS and exposed to NNK.…”

Fecal metabolomics combined with 16S rRNA gene sequencing to analyze the effect of Jiaotai pill intervention in type 2 diabetes mellitus rats

“…As the abundance of Firmicutes decreases, these protective effects are weakened, which is likely to induce the occurrence of type 2 diabetes mellitus. The increase in abundance of Bacteroidetes and the disturbance of lipid and energy metabolism further accelerate gut microbiota disorders and contribute to the development of type 2 diabetes mellitus ( 22 ). Bacteroidetes accelerate the secretion of lipopolysaccharides and thus cause insulin resistance ( 23 ).…”

Endothelial dysfunction, platelet hyperactivity, hypertension, and the metabolic syndrome: molecular insights and combating strategies

“…In addition, garlic’s organosulfur derivatives reduce inflammation. The garlic organosulfur compounds were found to effectively reduce dyslipidemia and liver fat by boosting taurine levels and encouraging hepatic fatty acid- oxidation which helps in maintaining healthy glucose levels due to its ability to inhibit DPP-4 and hepatic gluconeogenesis ( 248 ). Garlic can cure MetS naturally because its thiol groups fight ROS-mediated inflammation.…”